Eukaryotic DNA replication: a single-molecule approach to the study of yeast replication on chromatin

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CAF-1 deposits newly synthesized histones during DNA replication using distinct mechanisms on the leading and lagging strands

Autores: Clément Rouillon; Bruna V Eckhardt; Leonie Kollenstart; Fabian Gruss; Alexander E E Verkennis; Inge Rondeel; Peter H L Krijger; Giulia Ricci; Alva Biran; Theo van Laar; Charlotte M Delvaux de Fenffe; Georgiana Luppens; Pascal Albanese; Koichi Sato; Richard A Scheltema; Wouter de Laat; Puck Knipscheer; Nynke H Dekker; Anja Groth; Francesca Mattiroli
Publicado en: Nucleic acids research, 2023, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkad171

A Biophysics Toolbox for Reliable Data Acquisition and Processing in Integrated Force–Confocal Fluorescence Microscopy

Autores: Zhaowei Liu, Edo van Veen, Humberto Sánchez, Belén Solano, Francisco J. Palmero Moya, Kaley A. McCluskey, Daniel Ramírez Montero, Theo van Laar, and Nynke H. Dekker
Publicado en: ACS Photonics, 2024, ISSN 2330-4022
Editor: American Chemical Society
DOI: 10.1021/acsphotonics.3c01739

De novo fabrication of custom-sequence plasmids for the synthesis of long DNA constructs with extrahelical features

Autores: Daniel Ramírez Montero, Zhaowei Liu, Nynke H. Dekker
Publicado en: Biophysical Journal, 2023, ISSN 0006-3495
Editor: Biophysical Society
DOI: 10.1016/j.bpj.2023.11.008

DNA origins retain mobile licensing proteins

Autores: Humberto Sánchez, Kaley McCluskey, Theo van Laar, Edo van Veen, Filip M. Asscher, Belén Solano, John F.X. Diffley and Nynke H. Dekker
Publicado en: Nature Communications, Edición 12, 2021, Página(s) 1908, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-22216-x

Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation

Autores: Daniel Ramírez Montero; Humberto Sánchez; Edo van Veen; Theo van Laar; Belén Solano; John F. X. Diffley; Nynke H. Dekker
Publicado en: Nature Communications, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1101/2022.12.23.521684

A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint

Autores: Humberto Sánchez; Zhaowei Liu; Edo van Veen; Theo van Laar; John F. X. Diffley; Nynke H. Dekker
Publicado en: Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023), 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1101/2023.05.18.541303

Global correction of optical distortions in multicolor single-molecule microscopy using Zernike polynomial gradients

Autores: Kaley McCluskey; Edo van Veen; Jelmer Cnossen; Wouter Wesselink; Filip M. Asscher; Carlas Smith; Nynke H. Dekker
Publicado en: Optics Express, 2021, Página(s) 42251-42264, ISSN 1094-4087
Editor: Optical Society of America
DOI: 10.1364/oe.445230

Three-dimensional localization microscopy with increased axial precision through TIRF angle modulation

Autores: Daniel Fan; Jelmer Cnossen; Shih-Te Hung; Dimitri Kromm; Nynke H. Dekker; Gerard J. Verbiest; Carlas S. Smith
Publicado en: Optics Communications, 2023, Página(s) 129548, ISSN 0030-4018
Editor: Elsevier BV
DOI: 10.1016/j.optcom.2023.129548

Principles and best practices of optimizing a micromirror-based multicolor TIRF microscopy system

Autores: Kaley McCluskey; Nynke H. Dekker
Publicado en: Optics Communications, 2023, Página(s) 129474, ISSN 0030-4018
Editor: Elsevier BV
DOI: 10.1101/2023.03.27.534330

Physics meets biology: The joining of two forces to further our understanding of cellular function

Autores: Michelle D. Wang, Mario Nicodemi, Nynke H. Dekker, Thomas Gregor, David Holcman, Antoine M. van Oijen, and Suliana Manley,
Publicado en: Molecular Cell, 2021, Página(s) 3033-3037, ISSN 1097-2765
Editor: Cell Press
DOI: 10.1016/j.molcel.2021.07.009

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