Descrizione del progetto
Comprendere il ruolo dello spazio extracellulare cerebrale
Quanto conosciamo dell’interazione tra lo spazio extracellulare (ECS, Extracellular Space) cerebrale e l’attività neuronale? Il progetto SynECS, finanziato dall’UE, rafforzerà la nostra comprensione rivelando le caratteristiche biofisiche dello spazio extracellulare cerebrale attorno alle sinapsi durante l’attività neuronale. SynECS impiega un metodo innovativo di imaging ottico su nanoscala basato sul movimento di nanotubi di carbonio a parete singola nell’intricato labirinto dei canali dello spazio extracellulare cerebrale. Questi nanostrumenti possono risolvere dettagli morfologici e reologici su nanoscala, fornendo una definizione senza precedenti dello spazio extracellulare attorno alle sinapsi. SynECS rappresenta un progetto chiave per sostenere l’attività dei circuiti cerebrali in condizioni sia fisiologiche che patologiche.
Obiettivo
The extracellular space (ECS) is a complex network of biomolecules that constitutes a key microenvironment for cellular communication, homeostasis, and clearance of toxic metabolites. In the brain, signalling molecules, neuromodulators, and nutrients transit via the ECS, therefore mediating the communication between cells. To date, understanding the role of the ECS in modulating neuronal communication represents a knowledge frontier in brain research. This limit has conceptual and methodological roots: the complex 3-D dynamical organization of the ECS and the current lack of dedicated relevant investigation tools. The aim of this project is to combine several innovative nanotechnological approaches to reveal the morphological and rheological properties of the brain extracellular space around synapses during neuronal communication processes. To decrypt the intimate interplay between ECS and synapses in neuronal communication, this Action proposes to use innovative optical nanoimaging and nanostimulation methodologies based on nano-probes (single-wall carbon nanotubes, SWCNTs, and gold nanorods, Au NRs). Au NRs will be used to stimulate individual neurons in hippocampal cultured organotypic slices, while SWCNTs will image and unveil the rheological characteristics of the ECS around synapses. The outcomes of this project will bring us a step closer to fully understand chemical-based neural communication and synaptic connectivity.
Campo scientifico
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF-EF-RI - RI – Reintegration panelCoordinatore
75794 Paris
Francia