Novel high speed and high resolution microscopy setup for cytoarchitectonic studies of mesoscale sized human brain tissues, healthy and affected by Focal Cortical Dysplasia

Project Information

MesoBrainMicr

Grant agreement ID: 793849

DOI

10.3030/793849

Project closed

EC signature date 2 March 2018

Start date 1 October 2018

End date 30 September 2020

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 180 277,20

EU contribution

€ 180 277,20

Coordinated by

UNIVERSITA DEGLI STUDI DI FIRENZE
Italy

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Conference proceedings (6)

High-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions

Author(s): G. Mazzamuto, L. Silvestri, G. Sancataldo, V. Gavryusev, M. Scardigli, I. Costantini, and F. S. Pavone
Published in: Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, Issue PC1238509, 2023
Publisher: SPIE
DOI: 10.1117/12.2650374

Optimized dual-view light sheet microscopy for large area cleared specimens

Author(s): V. Gavryusev, G. Mazzamuto, G. Sancataldo, L. Pesce, A. Laurino, M. Scardigli, I. Costantini, L. Silvestri, and F. S. Pavone
Published in: Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020
Publisher: RMS
DOI: 10.22443/rms.lsfm2020.43

Swift light sheet volumetric charting of large human brain portions

Author(s): Vladislav Gavryusev, Luca Pesce, Annunziatina Laurino, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone
Published in: Biophotonics Congress: Biomedical Optics 2020 (Translational, Microscopy, OCT, OTS, BRAIN), 2020, Page(s) JW5A.3, ISBN 978-1-943580-74-3
Publisher: OSA
DOI: 10.1364/translational.2020.jw5a.3

Fast volumetric mapping of human brain slices

Author(s): Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone
Published in: Neurophotonics, 2020, Page(s) 10, ISBN 9781510634930
Publisher: SPIE
DOI: 10.1117/12.2555644

An AOD breakthrough for volumetric 2P optogenetic applications

Author(s): P. Ricci, M. Marchetti, M. Sorelli, L. Turrini, F. Resta, V. Gavryusev, G. de Vito, G. Sancataldo, F. Vanzi, L. Silvestri, and F. S. Pavone
Published in: Proc. SPIE 12144, Biomedical Spectroscopy, Microscopy, and Imaging II, Issue PC1214406, 2022
Publisher: SPIE
DOI: 10.1117/12.2620313

Fast volumetric mapping of human brain slices

Author(s): Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone
Published in: Neural Imaging and Sensing 2020, 2020, Page(s) 10, ISBN 9781510632165
Publisher: SPIE
DOI: 10.1117/12.2544133

Peer reviewed articles (9)

Fast multi-directional DSLM for confocal detection without striping artifacts

Author(s): Pietro Ricci; Giuseppe Sancataldo; Vladislav Gavryusev; Alessandra Franceschini; Marie Caroline Müllenbroich; Ludovico Silvestri; Francesco Saverio Pavone
Published in: Biomedical Optics Express, Issue 11/6, 2020, Page(s) 3111-3124, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/BOE.390916

Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy

Author(s): Giuseppe de Vito, Pietro Ricci, Lapo Turrini, Vladislav Gavryusev, Caroline Müllenbroich, Natascia Tiso, Francesco Vanzi, Ludovico Silvestri, Francesco Saverio Pavone
Published in: Biomedical Optics Express, Issue 11/8, 2020, Page(s) 4651, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.396388

3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

Author(s): Pesce, Luca and Scardigli, Marina and Gavryusev, Vladislav and Laurino, Annunziatina and Mazzamuto, Giacomo and Brady, Niamh and Sancataldo, Giuseppe and Silvestri, Ludovico and Destrieux, Christophe and Hof, Patrick R. and Costantini, Irene and Pavone, Francesco S.
Published in: Communications Biology, Issue 5/1, 2022, Page(s) 447, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-022-03390-0

A cellular resolution atlas of Broca’s area

Author(s): I. Costantini, L. Morgan, J. Yang, Y. Balbastre, D. Varadarajan, L. Pesce, M. Scardigli, G. Mazzamuto, V. Gavryusev, F.M. Castelli, M. Roffilli, L. Silvestri, J. Laffey, S. Raia, M. Varghese, B. Wicinski, S. Chang, A.C. I-Chun, H. Wang, D. Cordero, M. Vera, J. Nolan, K. Nestor, J. Mora, J.E. Iglesias, E.G. Pallares, K. Evancic, J. Augustinack, M. Fogarty, A.V. Dalca, M. Frosch, C. Magnain, R. Fros
Published in: Science Advances, Issue 9, 2023, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adg3844

Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts

Author(s): Giuseppe Sancataldo, Vladislav Gavryusev, Giuseppe de Vito, Lapo Turrini, Massimiliano Locatelli, Chiara Fornetto, Natascia Tiso, Francesco Vanzi, Ludovico Silvestri, Francesco Saverio Pavone
Published in: Frontiers in Neuroanatomy, Issue 13, 2019, ISSN 1662-5129
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnana.2019.00007

Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector

Author(s): Vladislav Gavryusev, Giuseppe Sancataldo, Pietro Ricci, Alberto Montalbano, Chiara Fornetto, Lapo Turrini, Annunziatina Laurino, Luca Pesce, Giuseppe de Vito, Natascia Tiso, Francesco Vanzi, Ludovico Silvestri, Francesco S. Pavone
Published in: Journal of Biomedical Optics, Issue 24/10, 2019, Page(s) 1, ISSN 1083-3668
Publisher: S P I E - International Society for Optical Engineering
DOI: 10.1117/1.JBO.24.10.106504

Removing striping artifacts in light-sheet fluorescence microscopy: A review

Author(s): P. Ricci, V. Gavryusev, C. Müllenbroich, L. Turrini, G. de Vito, L. Silvestri, G. Sancataldo, and F. S. Pavone
Published in: Progress in Biophysics and Molecular Biology, Issue 168, 2022, Page(s) 52-65, ISSN 1873-1732
Publisher: Elsevier
DOI: 10.1016/j.pbiomolbio.2021.07.003

Power-effective scanning with AODs for 3D optogenetic applications

Author(s): P. Ricci, M. Marchetti, M. Sorelli, L. Turrini, F. Resta, V. Gavryusev, G. de Vito, G. Sancataldo, F. Vanzi, L. Silvestri, and F. S. Pavone
Published in: Journal of Biophotonics, Issue 15/4, 2022, Page(s) e202100256, ISSN 1864-0648
Publisher: Wiley GmbH
DOI: 10.1002/jbio.202100256

Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques

Author(s): Marina Scardigli; Marina Scardigli; Luca Pesce; Luca Pesce; Niamh Brady; Giacomo Mazzamuto; Giacomo Mazzamuto; Vladislav Gavryusev; Vladislav Gavryusev; Ludovico Silvestri; Ludovico Silvestri; Ludovico Silvestri; Patrick R. Hof; Christophe Destrieux; Irene Costantini; Irene Costantini; Irene Costantini; Francesco S. Pavone; Francesco S. Pavone; Francesco S. Pavone
Published in: Frontiers in Neuroanatomy, Issue 11, 2021, Page(s) 90, ISSN 1662-5129
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnana.2021.752234