BioHybrid Optoelectronic Materials

Projektinformationen

BiHyOMat

ID Finanzhilfevereinbarung: 794536

DOI

10.3030/794536

Projekt abgeschlossen

EK-Unterschriftsdatum 7 März 2018

Startdatum 1 Juni 2018

Enddatum 20 Juni 2020

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten

€ 191 325,60

EU-Beitrag

€ 191 325,60

Koordiniert durch

AALTO KORKEAKOULUSAATIO SR
Finland

Periodic Reporting for period 1 - BiHyOMat (BioHybrid Optoelectronic Materials)

Berichtszeitraum: 2018-06-01 bis 2020-05-31

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

BiHyOMat is an ambitious research at the interface of chemistry, physics and biology, aiming to produce novel high-end materials for medical and environmental applications. The reversible conjugation of proteins and light absorbing molecules will combine the biocompatibility and defined structure of the former with the light-induced properties of the later. In particular, fluorescence and generated of reactive oxygen species have extensive application in sensors, photodynamic therapy or waste-water treatment.

The overall objectives are:
- Develop and validate new synthetic approaches towards monodimensional protein aggregates. This morphology will enhance the contact with the media, increasing the efficiency of the material.
- Create novel morphologies as scaffold to conjugate with magnetic nanoparticles or create metal nanoparticles.

The BiHyOMat project yielded three publications in high impact factor journals, exploring the preparation of anisotropic biohybrids with optical properties. The scientific output has generated the initial drive for a promising scientific career for the researcher. The action provided the necessary tools for career development: a wide and multidisciplinary collaboration network, teaching skills, group management and public outreach.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

At first, highly-charged molecules were synthesized and employed as molecular glue for protein electrostatic assembly. These molecules were employed to direct the rod-like tobacco mosaic virus (TMV) protein as scaffold for anisotropic materials (E. Anaya-Plaza et al., Adv. Mater. 2019). The preparation of high aspect‐ratio fibers was achieved by step‐wise decrease of the NaCl concentration in the media. This electrostatically driven assembly is fully reversible upon NaCl addition. The hybrids where structurally characterized by means of small-angle X-ray scattering (SAXS) and microscopy, including cryo-EM reconstruction. The oxidizing activity was tested by irradiating the sample at adequate wavelengths, photogenerating reactive oxygen species. The hybrids showed remarkable structural stability, allowing their incorporation in continuous-flow sets-up that performed resiliently over 10 cycles with a total irradiation time of 20 minutes, which proves their ability to perform in water remediation technologies.
Other morphologies were explored, employing toroidal proteins. Conjugation with cationic dyes was achieved, yielding rod-like aggregates that present inner pores able to undergo minerallization or external decoration.

The project's results were diseminated in the scientific community and the general public in form of participation in congresses, press releases and open public meetings.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The BiHyOMat project achieved, for the first time, anisotropic protein assemblies through electrostatic interaction. The resulting fibers proved their robustness and photoactivity in continuous flow set-up, in an unprecedented approach for water remediation. Additionally, toroidal protein aggregates have been achieved, resulting in porous materials for their exploitation in nanoparticle fabrication.

Abstract figure