Objective
African trypanosomes are flagellated parasitic protozoans that cause the devastating, lethal disease sleeping sickness, as well as causing economically damaging livestock infections across sub-Saharan Africa. Trypanosomiasis was targeted for elimination by the WHO and others in the London declaration by 2020. Trypanosomes are transmitted by the bite of an infected tsetse fly, which restricts their geographic distribution. In the salivary gland of the fly, parasites coat their surface with approximately 10 million copies of a single variant surface glycoprotein (VSG) in a single layer which is protective against the mammalian immune system and prepares trypanosomes for infectivity. This VSG is expressed in a mono-allelic fashion from a specialized telomeric expression site. VSG coat is an important virulence mechanism; without this coat, parasites are rapidly lysed by mammalian complement. This project will address a fundamental question in trypanosome biology: How do African trypanosomes express a single VSG in its coat? I will address this questions primarily using single cell RNA-seq. First, I will establish an inDrops pipeline at the Institut Pasteur in collaboration with Prof. Andrew Griffiths at ESPCI (Paris) using cultured T. brucei, which will allow me to parameterize this novel single cell RNA-seq pipeline. Then using this protocol, I will generate single cell transcriptomes from tsetse fly derived parasites, constructing a gene expression profile for development of trypanosome infectivity. inDrops is a truly disruptive technology that allows massively parallel indexing of cellular mRNA, allowing transcriptomic analysis of thousands of single cells. These finding will have broad significance in the field; firstly, they will provide the first high-resolution single-cell transcriptome of this pathogen, and they will constitute a significant advance in our understanding of gene expression and the acquisition of infectivity in African trypanosomes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine psychiatry sleep disorders
- medical and health sciences basic medicine immunology
- agricultural sciences animal and dairy science domestic animals animal husbandry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2017
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75724 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.