Project description DEENESFRITPL Utilising kidney organoids in the search for new diabetic nephropathy therapies Nephropathy is a major diabetes morbidity that often progresses to end-stage renal disease as there are no available treatments apart from glycaemic and blood pressure control strategies. To address this limitation, the EU-funded EPIORGABOLISM project is working on a novel diabetic nephropathy model that has the potential to assist future drug discovery efforts. Researchers will use human embryonic stem cells to derive kidney organoids and study how the metabolic alterations in diabetes can epigenetically reprogramme kidney cells and cause fibrosis. Results will provide important insight into disease pathology and progression and help identify novel therapeutic targets. Show the project objective Hide the project objective Objective Diabetic Nephropathy is the leading cause of end-stage renal disease (ESRD). To date, treatment of DN is mainly based on drugs acting on glycaemic and blood pressure control, as there is no validated therapy able to stop the progression towards renal failure. One of the main impediments for developing new therapies for DN has been the lack of a good preclinical model which can recapitulate important functional, structural, and molecular features of advanced human diabetic kidney disease. Here, we aim to develop a DN modelling using human Embryonic Stem Cell (hESC) derived kidney organoids which can recapitulate the in vivo architecture, functionality, and genetic signature of DN. Due to the increasing evidences that links aberrant DNA methylation with kidney fibrosis and metabolic reprograming in DN, we hypothesize that early DN progression is promoted by the metabolic alterations occurring in diabetic patients, resulting in the epigenetic reprogramming of kidney proximal tubular epithelial cells (KPTCs). Based in my background in the fields of metabolism and diabetes, together with the expertise of Dr. Montserrat in the field of somatic reprograming, DN and tissue regeneration/differentiation, this proposal seeks to:1) Elucidate the methylation status of the promoters/enhancers of genes encoding enzymes and regulators of fibrosis and fatty acid oxidation in proximal tubular cells obtained from diabetic patients at different stages of DN progression 2) Establish two in vitro models using hESC-derived kidney organoids; an engineered DN in vitro model, using CRISPR Cas9 to mimic the epigenome signature of DN patients and a physiological DN in vitro model which mimic the diabetic physiological chemistry. The information gained from this DN modelling will offer improved insight into disease pathology and progression. Moreover, it may also serve as a tool for drug discovery to identify therapeutic targets for DN. Fields of science medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverymedical and health sciencesclinical medicineendocrinologydiabetesdiabetic nephropathymedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicinepathologymedical and health sciencesclinical medicinenephrologykidney diseases Keywords Methylation CRISPR Cas9 Diabetic Nephropathy Kidney Organoids Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2017 - Individual Fellowships Call for proposal H2020-MSCA-IF-2017 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA Net EU contribution € 158 121,60 Address CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona Spain See on map Region Este Cataluña Barcelona Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 158 121,60
