Objective
Cell-cell interaction mechanisms play a role in many fundamental biological processes from symbiotic interactions of fungi and bacteria with their host plants to fertilization both in animals and plants. In plant reproduction, pollen tube (PT) reception is a cell-cell communication process allowing the recognition of the male gametophyte (the PT) by the female gametophyte necessary for fertilization. To investigate this process, the host lab used interspecific crosses between certain Arabidopsis thaliana accessions and A.lyrata pollen, where most PTs are not recognized, causing a failure in sperm release and thus fertilization. In contrast, several other A.thaliana accessions almost perfectly recognize interspecific PTs. Preliminary results identified the gene At5g62150 as a key player for species-specific PT recognition. At5g62150 codes for a LysM-domain containing glycan-binding peptide, pointing at glycosylation patterns for the distinction of intra- and interspecific PTs, a mechanism already known to be important in symbiosis and immunity. It was found that differences in the regulation of At5g62150 in distinct species is responsible for recognition, as the amino acid sequence does not differ between A. thaliana other accessions. With this proposal, we will first attest and manipulate the expression profile of At5g62150 in different accessions to correlate its regulation with its function. Then, we propose to dissect the regulation of At5g62150 in different ways: identification and study of the essential cis-regulatory element for regulation and Y1H screening and Reverse ChIP to find trans-acting regulators. The output will not only pave the way for the understanding and manipulating crosses barriers in plants, which is highly relevant for plant breeding, and shed light on cell-cell communication processes in general, but also will establish a novel, powerful instrument (Reverse ChIP) to investigate DNA-protein interaction in complex organisms.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules
- natural sciences biological sciences microbiology mycology
- natural sciences biological sciences biological behavioural sciences ethology biological interactions
- natural sciences chemical sciences organic chemistry amines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2017
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
8006 Zurich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.