Cypergenetic Tissue Engineering

Projektinformationen

CyGenTiG

ID Finanzhilfevereinbarung: 801041

DOI

10.3030/801041

Projekt abgeschlossen

EK-Unterschriftsdatum 3 Mai 2018

Startdatum 1 Oktober 2018

Enddatum 31 März 2024

Finanziert unter

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Gesamtkosten

€ 4 696 250,00

EU-Beitrag

€ 4 696 250,00

Koordiniert durch

ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
Germany

CORDIS bietet Links zu öffentlichen Ergebnissen und Veröffentlichungen von HORIZONT-Projekten.

Links zu Ergebnissen und Veröffentlichungen von RP7-Projekten sowie Links zu einigen Typen spezifischer Ergebnisse wie Datensätzen und Software werden dynamisch von OpenAIRE abgerufen.

Leistungen

Documents, reports (19)

Optimised switches 2. generation

Optimized and customized optical switches for spatio-temporal control of stem cell fate.

Optimised switches 1. generation

Optimized and customized optical switches for spatiotemporal control of stem cell fate

Construction plans for optogenetic synthetic modules

Construction plan for synthetic biological modules optogenetic sensors (WP1) inserted into them.

Final optimized image analysis

Final optimized image analysis modules.

Kick-off meeting

Report on the agenda, list of participants of the kick-off meeting

Image sets of optical- controlled stem cell differentiation

Image sets demonstrating control of stem cell differentiation by closed-loop optical control.

Improved image analysis

Improved image analysis modules

Organoid model

Optimal-control model for growth control of renal organoids.

Quantitative description of the optogenetic systems

Deliver a quantitative (mathematical) description of the optogenetic systems and provide an optimization strategy to meet the required quantitative and dynamic responses or 2D control of stem cells.

Web site and logo

Set up of a project web site and decide on a logo.

Image analysis modules

Initial version of image analysis modules. Construction and training of deep convolutional networks for the segmentation of the desired structures (2D-islands, differentiated/undifferentiated single cells and cap mesenchymes of kidneys).

Image sets of optical-controlled cell sheet morphogenesis

Image sets for the morphogenesis of simple cell sheets imposed by closedloop optical control

Synthetic modules controlling cell fate

Synthetic biological modules for locomotion, adhesion, proliferation, apoptosis and folding, with optogenetic sensors (WP1) inserted into them.

Model for cell islands

Optimalcontrol model for growth control of flat cell islands

Model for stem cell differentiation

Optimalcontrol model for spatial stem cell differentiation

Characterization of light-regulated gene expression systems

Fully characterization of light-regulated gene expression systems in terms of kinetics, dynamic range and sensitivity in HEK293 and 6TA3 cells.

Model predictive control algorithms

Model predictive control algorithms for growth control.

Tested optogenetic cells

Mammalian cell lines carrying one of the modules from D21 tested with allover illumination

Patterned illumination

Hardware and software for patterned illumination setup. DMD (“digital micromirror device”) based illumination system with multiple wavelengths and optimize it for mammalian cells. Automated procedures to align the coordinate systems of the sample and the DMD. Design of control interfaces to enable simple and reliable device operation.

Demonstrators, pilots, prototypes (1)

Image sets of optical- controlled polarization/ ureter production in renal organoids

Image sets and quantitative measurements of the optical closed-loop control of polarization/ ureter production in renal organoids.

Veröffentlichungen

Peer reviewed articles (21)

Synthetic morphology with agential materials

Autoren: Jamie Davies, Michael Levin
Veröffentlicht in: Nature Reviews Bioengineering, Ausgabe 1, 2023, Seite(n) 46-59, ISSN 2731-6092
Herausgeber: SpringerNature
DOI: 10.1038/s44222-022-00001-9

Engineering of Bidirectional, Cyanobacteriochrome-Based Light-Inducible Dimers (BICYCL)s

Autoren: Jang, Jaewan, Kun Tang, Jeffrey Youn, Sherin McDonald, Hannes M. Beyer, Matias D. Zurbriggen, Maruti Uppalapati, and G. Andrew Woolley
Veröffentlicht in: Nature Methods, Ausgabe 20, 2023, ISSN 1548-7091
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41592-023-01764-8

Cell-in-the-loop pattern formation with optogenetically emulated cell-to-cell signaling

Autoren: Melinda Liu Perkins, Dirk Benzinger, Murat Arcak, Mustafa Khammash
Veröffentlicht in: Nature Communications, Ausgabe 11/1, 2020, ISSN 2041-1723
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41467-020-15166-3

Unlocking the potential of optogenetics in microbial applications

Autoren: Moritz Benisch; Stephanie K Aoki; Mustafa Khammash
Veröffentlicht in: Current Opinion in Microbiology, 77, Ausgabe 6, 2024, ISSN 1369-5274
Herausgeber: Elsevier BV
DOI: 10.1016/j.mib.2023.102404

Single cell variability of CRISPR-Cas interference and adaptation

Autoren: Rebecca E McKenzie Emma M Keizer Jochem N A Vink Jasper van Lopik Ferhat Büke Vera Kalkman Christian Fleck Sander J Tans Stan J J Brouns
Veröffentlicht in: Molecular Systems Biology, 2022, ISSN 1744-4292
Herausgeber: Nature Publishing Group
DOI: 10.15252/msb.202110680

Optogenetics in Plants

Autoren: Christie JM and Zurbriggen MD
Veröffentlicht in: New Phytologist, Ausgabe 229,6, 2020, Seite(n) 3108-3115, ISSN 1469-8137
Herausgeber: Wiley
DOI: 10.1111/nph.17008

Exploiting natural chemical photosensitivity of anhydrotetracycline and tetracycline for dynamic and setpoint chemo-optogenetic control

Autoren: Armin Baumschlager, Marc Rullan, Mustafa Khammash
Veröffentlicht in: Nature Communications, Ausgabe 11/1, 2020, ISSN 2041-1723
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41467-020-17677-5

COLD REGULATED 27 and 28 are targets of CONSTITUTIVELY PHOTOMORPHOGENIC 1 and negatively affect phytochrome B signalling

Autoren: Nikolai Kahle, David J. Sheerin, Patrick Fischbach, Leonie‐Alexa Koch, Philipp Schwenk, Dorothee Lambert, Ryan Rodriguez, Konstantin Kerner, Ute Hoecker, Matias D. Zurbriggen, Andreas Hiltbrunner
Veröffentlicht in: The Plant Journal, Ausgabe 104/4, 2020, Seite(n) 1038-1053, ISSN 0960-7412
Herausgeber: Blackwell Publishing Inc.
DOI: 10.1111/tpj.14979

Agent-Based Models in Cellular Systems

Autoren: Jonas Pleyer, Christian Fleck
Veröffentlicht in: Frontiers of Physics - Biophysics, 2022, ISSN 2296-424X
Herausgeber: Frontiers Media
DOI: 10.3389/fphy.2022.968409

Platforms for Optogenetic Stimulation and Feedback Control

Autoren: Kumar, Sant, and Mustafa Khammash
Veröffentlicht in: Frontiers in Bioengineering and Biotechnology, Ausgabe 10, 2022, ISSN 2296-4185
Herausgeber: Frontiers
DOI: 10.3389/fbioe.2022.918917

Synthetic Biological Approaches for Optogenetics and Tools for Transcriptional Light‐Control in Bacteria

Autoren: Armin Baumschlager, Mustafa Khammash
Veröffentlicht in: Advanced Biology, Ausgabe 5/5, 2021, Seite(n) 2000256, ISSN 2701-0198
Herausgeber: Wiley
DOI: 10.1002/adbi.202000256

Synthetic Morphogenesis: introducing IEEE journal readers to programming living mammalian cells to make structures

Autoren: Jamie Davies
Veröffentlicht in: Proceedings of the IEEE, 2022, ISSN 1558-2256
Herausgeber: Institute of Electrical and Electronics Engineers
DOI: 10.1109/jproc.2021.3137077

Engineering AraC to make it responsive to light instead of arabinose

Autoren: Edoardo Romano, Armin Baumschlager, Emir Bora Akmeriç, Navaneethan Palanisamy, Moustafa Houmani, Gregor Schmidt, Mehmet Ali Öztürk, Leonard Ernst, Mustafa Khammash, Barbara Di Ventura
Veröffentlicht in: Nature Chemical Biology, Ausgabe 17/7, 2021, Seite(n) 817-827, ISSN 1552-4450
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41589-021-00787-6

Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors

Autoren: David Golonka, Patrick Fischbach, Siddhartha G. Jena, Julius R. W. Kleeberg, Lars-Oliver Essen, Jared E. Toettcher, Matias D. Zurbriggen, Andreas Möglich
Veröffentlicht in: Communications Biology, Ausgabe 2/1, 2019, ISSN 2399-3642
Herausgeber: Springer Nature
DOI: 10.1038/s42003-019-0687-9

The red edge: Bilin-binding photoreceptors as optogenetic tools and fluorescence reporters

Autoren: Tang K Beyer HM Zurbriggen MD Gärtner W
Veröffentlicht in: Chemical Reviews, 2021, ISSN 1520-6890
Herausgeber: ACS Publications
DOI: 10.1021/acs.chemrev.1c00194

Rapid Prototyping and Design of Cybergenetic Single-Cell Controllers

Autoren: Kumar, S., Rullan, M., & Khammash, M.
Veröffentlicht in: Nature Communications, Ausgabe 12, 2021, ISSN 2041-1723
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41467-021-25754-6

Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown)

Autoren: Tim Blomeier, Patrick Fischbach, Leonie‐Alexa Koch, Jennifer Andres, Miguel Miñambres, Hannes Michael Beyer, Matias Daniel Zurbriggen
Veröffentlicht in: Advanced Biology, Ausgabe 5/5, 2021, Seite(n) 2000307, ISSN 2701-0198
Herausgeber: Wiley
DOI: 10.1002/adbi.202000307

Renal engineering: strategies to address the problem of the ureter

Autoren: Davies JA, Elhendawi M, Palakkan A, Sallam M
Veröffentlicht in: Current Opinion in Biomedical Engineering, 2021, ISSN 2468-4511
Herausgeber: Elsevier
DOI: 10.1016/j.cobme.2021.100334

Optogenetic control of gene expression in plants in the presence of ambient white light

Autoren: Rocio Ochoa-Fernandez, Nikolaj B Abel, Franz-Georg Wieland, Jenia Schlegel, Leonie-Alexa Koch, J Benjamin Miller, Raphael Engesser, Giovanni Giuriani, Simon M Brandl, Jens Timmer, Wilfried Weber, Thomas Ott, Rüdiger Simon, Matias D Zurbriggen
Veröffentlicht in: Nature Methods, Ausgabe 17 (7), 2020, Seite(n) 717-725, ISSN 1548-7091
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41592-020-0868-y

Diya – A universal light illumination platform for multiwell plate cultures

Autoren: Sant Kumar; Stanislav Anastassov; Stephanie K. Aoki; Johannes Falkenstein; Ching-Hsiang Chang; Timothy Frei; Peter Buchmann; Paul Argast; Mustafa Khammash
Veröffentlicht in: iScience, 26 (10), Ausgabe 17, 2024, ISSN 2589-0042
Herausgeber: Elsevier BV
DOI: 10.1016/j.isci.2023.107862

Stratified tissue biofabrication by rotational internal flow layer engineering

Autoren: Ian Holland; Wenmiao Shu; Jamie A Davies
Veröffentlicht in: Holland , I , Shu , W & Davies , J A 2023 , ' Stratified tissue biofabrication by rotational internal flow layer engineering ' , Biofabrication . https://doi.org/10.1088/1758-5090/ace2ed, Ausgabe 17, 2023, ISSN 1758-5082
Herausgeber: IOS Press
DOI: 10.1088/1758-5090/ace2ed