Chromatin organization PROfiling with high-throughput super-resolution microscopy on a CHIP

Resultado final

Data management plan

Data management plan. Determination of research data accessible to the public

Kick-off meeting organization

Organization of project kick-off meeting to nominate the steering committee organize the WPs activity

LSF image simulator

LSF image simulator: realistic LSF datasets.

Third year annual meeting organization

Organization of annual meeting with project partners steering and mentoring committee

Software for phenotype profiling

Software suite dedicated to phenotype profiling

First Year annual meeting organization

Organization of project annual meeting with steering and mentoring committee to monitor the progress of the WPs activity

Real-time demodulation software of structured light

Demodulation software real time demodulation of structured light

Second year annual meeting organization

Organization of annual meeting with project partners steering and mentoring committee

Dissemination and exploitation plan

Report on the possible dissemination and exploitation of results.

High throughput imaging

Validation of imaging in a fluidic channel at high throughput

Phase shifter performance assesmet

Characterization of the phase shifter integrated on chip Maximum frequency will be retrieved

Updated dissemination and exploitation plan

Update of the plan containing actions for dissemination and exploitation of results on the basis of the project development

Benchmarking virtual instrumentation

Benchmarking virtual instrumentation select suitable microscopic setups

Cancer cell high throughput imaging

Retrieving high quality imaging of thousands of cancer cellssample

Optimal imaging settings for SIM

Optimal imaging settings for SIM and superresolution SIM characterization of image quality obtained by using structured light at different frequencies phases and angles

Web Page and Logo

Web Page. Design and make accessible a web page for external and internal access. Project Logo designed. Available at Month 2 and constantly updated during the project.

Publicaciones

GPU-accelerated real-time reconstruction in Python of three-dimensional datasets from structured illumination microscopy with hexagonal patterns

Autores: Hai Gong, Wenjun Guo and Mark A. A. Neil
Publicado en: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2021, ISSN 1364-503X
Editor: Royal Society of London
DOI: 10.1098/rsta.2020.0162

Detecting differences of fluorescent markers distribution in single cell microscopy: textural or pointillist feature space?

Autores: Ali Ahmad, Carole Frindel and David Rousseau
Publicado en: Frontiers in Robotics and AI, 2020, ISSN 2296-9144
Editor: Frontiers
DOI: 10.3389/frobt.2020.00039

Laser-Assisted Etching of EagleXG Glass by Irradiation at Low Pulse-Repetition Rate

Autores: Roberto Memeo, Mattia Bertaso, Roberto Osellame, Francesca Bragheri and Andrea Crespi
Publicado en: APPLIED SCIENCES, 2022, ISSN 2076-3417
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/app12030948

Strategies for improved temporal response of glass-based optical switches

Autores: Calvarese, Matteo; Paiè, Petra; Ceccarelli, Francesco; Sala, Federico; Bassi, Andrea; Osellame, Roberto; Bragheri, Francesca
Publicado en: Scientific Reports, Vol 12, Iss 1, Pp 1-9 (2022), Edición 2, 2022, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-021-04218-3

Effects of Thermal Annealing on Femtosecond Laser Micromachined Glass Surfaces

Autores: Federico Sala, Petra Paié, Rebeca Martínez Vázquez, Roberto Osellame and Francesca Bragheri
Publicado en: Micromachines, 2021, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020180

MicroVIP: Microscopy image simulation on the Virtual Imaging Platform

Autores: Ali Ahmad; Ali Ahmad; Guillaume Vanel; Sorina Camarasu-Pop; Axel Bonnet; Carole Frindel; David Rousseau
Publicado en: SoftwareX, Vol 16, Edición 1, 2021, Página(s) 100854, ISSN 0950-5849
Editor: Elsevier BV
DOI: 10.1016/j.softx.2021.100854

Femtosecond-laser-written optofluidics in alumino-borosilicate glass

Autores: Andrea Crespi, Roberto Osellame, Francesca Bragheri
Publicado en: Optical Materials: X, Edición 4, 2019, Página(s) 100042, ISSN 2590-1478
Editor: Elsevier
DOI: 10.1016/j.omx.2019.100042

Automatic imaging of Drosophila embryos with light sheet fluorescence microscopy on chip

Autores: Roberto Memeo, Petra Paiè, Federico Sala, Michele Castriotta, Chiara Guercio, Thomas Vaccari, Roberto Osellame, Andrea Bassi and Francesca Bragheri
Publicado en: Journal of Biophotonics, 2020, ISSN 1864-063X
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/jbio.202000396

Improving axial resolution in SIM using deep learning

Autores: Boland, Miguel; Cohen, Edward A. K.; Flaxman, Seth; Neil, Mark A. A.
Publicado en: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Edición 4, 2021, ISSN 1364-503X
Editor: Royal Society of London
DOI: 10.1098/rsta.2020.0298

High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip

Autores: Sala, Federico; Castriotta, Michele; Paiè, Petra; Farina, Andrea; D’Annunzio, Sarah; Zippo, Alessio; Osellame, Roberto; Bragheri, Francesca; Bassi, Andrea
Publicado en: Biomed Opt Express, Edición 5, 2020, ISSN 2156-7085
Editor: The Optical Society
DOI: 10.1364/boe.393892

Editorial for the Special Issue on New Trends and Applications in Femtosecond Laser Micromachining

Autores: Francesca Bragheri, Petra Paiè, Rebeca Martinez Vázquez
Publicado en: Micromachines, 2022, ISSN 2072-666X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi13020150

Optofluidic lab-on-chips for high throughput 3D imaging of cells and tissues

Autores: Federico Sala, Petra Paiè, Roberto Memeo, Roberto Osellame, Andrea Farina, Andrea Bassi, Francesca Bragheri
Publicado en: EPJ Web of Conferences, Edición 215, 2019, Página(s) 11002, ISSN 2100-014X
Editor: EDP Sciences
DOI: 10.1051/epjconf/201921511002

Deep learning based detection of cells in 3D light sheet fluorescence microscopy

Autores: Ali Ahmad, Carole Frindel, Pejman Rasti, David Sarrut, David Rousseau
Publicado en: Quantitative BioImaging Conference, 2019
Editor: None

Détection de différence de densité de marqueurs fluorescents en microscopie superrésolue : approche pointilliste ou texturale ?

Autores: Ali Ahmad, Carole Frindel, David Rousseau
Publicado en: 2019
Editor: GRETSI, Groupe d’Etudes du Traitement du Signal et des Images

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