Chromatin organization PROfiling with high-throughput super-resolution microscopy on a CHIP

Projektinformationen

PROCHIP

ID Finanzhilfevereinbarung: 801336

DOI 10.3030/801336

Projekt abgeschlossen

EK-Unterschriftsdatum 19 April 2018

Startdatum 1 September 2018

Enddatum 28 Februar 2022

Finanziert unter

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Gesamtkosten € 2 496 525,00

EU-Beitrag € 2 496 525,00

Koordiniert durch

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

Leistungen

Open Research Data Pilot (1)

Data management plan

Data management plan. Determination of research data accessible to the public

Other (7)

Kick-off meeting organization

Organization of project kick-off meeting to nominate the steering committee organize the WPs activity

LSF image simulator

LSF image simulator: realistic LSF datasets.

Third year annual meeting organization

Organization of annual meeting with project partners steering and mentoring committee

Software for phenotype profiling

Software suite dedicated to phenotype profiling

First Year annual meeting organization

Organization of project annual meeting with steering and mentoring committee to monitor the progress of the WPs activity

Real-time demodulation software of structured light

Demodulation software real time demodulation of structured light

Second year annual meeting organization

Organization of annual meeting with project partners steering and mentoring committee

Documents, reports (7)

Dissemination and exploitation plan

Report on the possible dissemination and exploitation of results.

High throughput imaging

Validation of imaging in a fluidic channel at high throughput

Phase shifter performance assesmet

Characterization of the phase shifter integrated on chip Maximum frequency will be retrieved

Updated dissemination and exploitation plan

Update of the plan containing actions for dissemination and exploitation of results on the basis of the project development

Benchmarking virtual instrumentation

Benchmarking virtual instrumentation select suitable microscopic setups

Cancer cell high throughput imaging

Retrieving high quality imaging of thousands of cancer cellssample

Optimal imaging settings for SIM

Optimal imaging settings for SIM and superresolution SIM characterization of image quality obtained by using structured light at different frequencies phases and angles

Websites, patent fillings, videos etc. (1)

Web Page and Logo

Web Page. Design and make accessible a web page for external and internal access. Project Logo designed. Available at Month 2 and constantly updated during the project.

Veröffentlichungen

Peer reviewed articles (10)

GPU-accelerated real-time reconstruction in Python of three-dimensional datasets from structured illumination microscopy with hexagonal patterns

Autoren: Hai Gong, Wenjun Guo and Mark A. A. Neil
Veröffentlicht in: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2021, ISSN 1364-503X
Herausgeber: Royal Society of London
DOI: 10.1098/rsta.2020.0162

Detecting differences of fluorescent markers distribution in single cell microscopy: textural or pointillist feature space?

Autoren: Ali Ahmad, Carole Frindel and David Rousseau
Veröffentlicht in: Frontiers in Robotics and AI, 2020, ISSN 2296-9144
Herausgeber: Frontiers
DOI: 10.3389/frobt.2020.00039

Laser-Assisted Etching of EagleXG Glass by Irradiation at Low Pulse-Repetition Rate

Autoren: Roberto Memeo, Mattia Bertaso, Roberto Osellame, Francesca Bragheri and Andrea Crespi
Veröffentlicht in: APPLIED SCIENCES, 2022, ISSN 2076-3417
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/app12030948

Strategies for improved temporal response of glass-based optical switches

Autoren: Calvarese, Matteo; Paiè, Petra; Ceccarelli, Francesco; Sala, Federico; Bassi, Andrea; Osellame, Roberto; Bragheri, Francesca
Veröffentlicht in: Scientific Reports, Vol 12, Iss 1, Pp 1-9 (2022), Ausgabe 2, 2022, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-021-04218-3

Effects of Thermal Annealing on Femtosecond Laser Micromachined Glass Surfaces

Autoren: Federico Sala, Petra Paié, Rebeca Martínez Vázquez, Roberto Osellame and Francesca Bragheri
Veröffentlicht in: Micromachines, 2021, ISSN 2072-666X
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12020180

MicroVIP: Microscopy image simulation on the Virtual Imaging Platform

Autoren: Ali Ahmad; Ali Ahmad; Guillaume Vanel; Sorina Camarasu-Pop; Axel Bonnet; Carole Frindel; David Rousseau
Veröffentlicht in: SoftwareX, Vol 16, Ausgabe 1, 2021, Seite(n) 100854, ISSN 0950-5849
Herausgeber: Elsevier BV
DOI: 10.1016/j.softx.2021.100854

Femtosecond-laser-written optofluidics in alumino-borosilicate glass

Autoren: Andrea Crespi, Roberto Osellame, Francesca Bragheri
Veröffentlicht in: Optical Materials: X, Ausgabe 4, 2019, Seite(n) 100042, ISSN 2590-1478
Herausgeber: Elsevier
DOI: 10.1016/j.omx.2019.100042

Automatic imaging of Drosophila embryos with light sheet fluorescence microscopy on chip

Autoren: Roberto Memeo, Petra Paiè, Federico Sala, Michele Castriotta, Chiara Guercio, Thomas Vaccari, Roberto Osellame, Andrea Bassi and Francesca Bragheri
Veröffentlicht in: Journal of Biophotonics, 2020, ISSN 1864-063X
Herausgeber: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/jbio.202000396

Improving axial resolution in SIM using deep learning

Autoren: Boland, Miguel; Cohen, Edward A. K.; Flaxman, Seth; Neil, Mark A. A.
Veröffentlicht in: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Ausgabe 4, 2021, ISSN 1364-503X
Herausgeber: Royal Society of London
DOI: 10.1098/rsta.2020.0298

High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip

Autoren: Sala, Federico; Castriotta, Michele; Paiè, Petra; Farina, Andrea; D’Annunzio, Sarah; Zippo, Alessio; Osellame, Roberto; Bragheri, Francesca; Bassi, Andrea
Veröffentlicht in: Biomed Opt Express, Ausgabe 5, 2020, ISSN 2156-7085
Herausgeber: The Optical Society
DOI: 10.1364/boe.393892

Non-peer reviewed articles (2)

Editorial for the Special Issue on New Trends and Applications in Femtosecond Laser Micromachining

Autoren: Francesca Bragheri, Petra Paiè, Rebeca Martinez Vázquez
Veröffentlicht in: Micromachines, 2022, ISSN 2072-666X
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi13020150

Optofluidic lab-on-chips for high throughput 3D imaging of cells and tissues

Autoren: Federico Sala, Petra Paiè, Roberto Memeo, Roberto Osellame, Andrea Farina, Andrea Bassi, Francesca Bragheri
Veröffentlicht in: EPJ Web of Conferences, Ausgabe 215, 2019, Seite(n) 11002, ISSN 2100-014X
Herausgeber: EDP Sciences
DOI: 10.1051/epjconf/201921511002

Conference proceedings (2)

Deep learning based detection of cells in 3D light sheet fluorescence microscopy

Autoren: Ali Ahmad, Carole Frindel, Pejman Rasti, David Sarrut, David Rousseau
Veröffentlicht in: Quantitative BioImaging Conference, 2019
Herausgeber: None

Détection de différence de densité de marqueurs fluorescents en microscopie superrésolue : approche pointilliste ou texturale ?

Autoren: Ali Ahmad, Carole Frindel, David Rousseau
Veröffentlicht in: 2019
Herausgeber: GRETSI, Groupe d’Etudes du Traitement du Signal et des Images