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The Extracellular Vesicle Foundry

Deliverables

Dissemination and communication plan

Dissemination and communication plan prepared

Optimal storage and shipment of nanosized EVs

Definition and report on optimal storage and shipment of nanosized EVs

SOPs for quality standard nanosized EVs from bovine milk

Standard Operating Procedures (SOPs) for quality standard nanosized EVs from bovine milk defined.

Colloidal characterization of nanosized EVs

Report on colloidal characterization of nanosized EVs

EV-surface interaction fundamentals

Report on EVsurface interaction fundamentals

SOPs for quality standard nanosized EVs from Ascaris media

Standard Operating Procedures (SOPs) for quality standard nanosized EVs from Ascaris media defined.

Library of nano-DLD surfaces

Library of nano-DLD surfaces fabricated.

Library of nanostructured surfaces non-lamellar lipid phases

Library of nanostructured surfaces made of non-lamellar lipid phases fabricated.

Library of nanostructured surfaces classified by EV interactions

Library of relevant nanostructured surfaces classified in terms of their interactions with the EVs

Internal and main external communication channels

Internal and main external communication channels defined and operating

Project website online and logo

Project website online and logo defined.

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Publications

Nanoscale structural and mechanical characterization of thin bicontinuous cubic phase lipid films

Author(s): Andrea Ridolfi, LucreziaCaselli, Costanza Montis, Tommy Nylander, DeboraBerti, Marco Brucale, Francesco Valle.
Published in: Colloids and Surfaces B: Biointerfaces, Volume 210, February 2022, 112231, 2022, ISSN 0927-7765
Publisher: Elsevier BV
DOI: 10.1016/j.colsurfb.2021.112231

Comparison of separation methods for immunomodulatory extracellular vesicles from helminths.

Author(s): Borup,A.,Boysen,A.T.,Ridolfi,A.,Brucale,M.,Valle,F.,Paolini,L.,Bergese,P.,&Nejsum,P.
Published in: Journal of Extracellular Biology, 2022;1:e41, 2022, Page(s) 1-17, ISSN 2768-2811
Publisher: John Wiley & Sons, Ltd
DOI: 10.1002/jex2.41

Nanoanalytical analysis of bisphosphonate-driven alterations of microcalcifications using a 3D hydrogel system and in vivo mouse model

Author(s): Ruiz JL, Hutcheson JD, Cardoso L, Bakhshian Nik A, Condado de Abreu A, Pham T, Buffolo F, Busatto S, Federici S, Ridolfi A, Aikawa M, Bertazzo S, Bergese P, Weinbaum S, Aikawa E.
Published in: Proceedings of the National Academy of Sciences, 118 (14), 2021, Page(s) 1811725118, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1811725118

Nanoparticles and organized lipid assemblies: from interaction to design of hybrid soft devices

Author(s): Marco Mendozza, Lucrezia Caselli, Annalisa Salvatore, Costanza Montis, Debora Berti
Published in: Soft Matter, 15/44, 2019, Page(s) 8951-8970, ISSN 1744-683X
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sm01601e

Cell Sorting Using Electrokinetic Deterministic Lateral Displacement.

Author(s): Bao Dang Ho; Jason P. Beech; Jonas O. Tegenfeldt
Published in: Micromachines, Issue 1, 4, 2020, Page(s) 2020 Dec 30;12(1):30, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010030

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Author(s): Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Published in: Nanoscale Horizons, 6/7, 2021, Page(s) 543-550, ISSN 2055-6756
Publisher: The Royal Society of Chemistry 2021
DOI: 10.1039/d1nh00012h

Deterministic Lateral Displacement: Challenges and Perspectives.

Author(s): Hochstetter A, Vernekar R, Austin RH, Becker H, Beech JP, Fedosov DA, Gompper G, Kim SC, Smith JT, Stolovitzky G, Tegenfeldt JO, Wunsch BH, Zeming KK, Krüger T, Inglis DW.
Published in: ACS Nano, 14(9), 2020, Page(s) 10784-10795, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.0c05186

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Author(s): Blendi Ura; Stefania Biffi; Lorenzo Monasta; Giorgio Arrigoni; Ilaria Battisti; Giovanni Di Lorenzo; Federico Romano; Michelangelo Aloisio; Fulvio Celsi; Riccardo Addobbati; Francesco Valle; Enrico Rampazzo; Marco Brucale; Andrea Ridolfi; Danilo Licastro; Giuseppe Ricci
Published in: Cancers, 2, 2021, Page(s) Cancers 2021, 13(14), 3639, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces

Author(s): Costanza Montis, Annalisa Salvatore, Francesco Valle, Lucia Paolini, Francesco Carlà, Paolo Bergese, Debora Berti
Published in: Journal of Colloid and Interface Science, 570, 2020, Page(s) 340-349, ISSN 0021-9797
Publisher: Academic Press
DOI: 10.1016/j.jcis.2020.03.014

AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles

Author(s): Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T. Boysen, Francesca Loria, Martijn J. C. van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H. M. Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Published in: Analytical Chemistry, 92/15, 2020, Page(s) 10274-10282, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.9b05716

Fluorescent Labeling of Helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach

Author(s): Anders T. Boysen, Bradley Whitehead, Allan Stensballe, Anna Carnerup, Tommy Nylander, Peter Nejsum
Published in: Biomedicines, 8/7, 2020, Page(s) 213, ISSN 2227-9059
Publisher: Biomedicines
DOI: 10.3390/biomedicines8070213

Regular Industrial Processing of Bovine Milk Impacts the Integrity and Molecular Composition of Extracellular Vesicles

Author(s): Marije Kleinjan, Martijn JC van Herwijnen, Sten FWM Libregts, RJ Joost van Neerven, Anouk L Feitsma, Marca HM Wauben
Published in: The Journal of Nutrition, 151/6, 2021, Page(s) 1416-1425, ISSN 0022-3166
Publisher: American Society for Nutritional Sciences
DOI: 10.1093/jn/nxab031

Cell Sorting Using Electrokinetic Deterministic Lateral Displacement

Author(s): Ho BD, Beech JP, Tegenfeldt JO
Published in: Micromachines, 12(1), 2020, Page(s) 30, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010030

Stiffness of Fluid and Gel Phase Lipid Nanovesicles: Weighting the Contributions of Membrane Bending Modulus and Luminal Pressurization.

Author(s): Andrea Ridolfi, Lucrezia Caselli, Matteo Baldoni, Costanza Montis, Francesco Mercuri, Debora Berti, Francesco Valle, and Marco Brucale
Published in: Langmuir 2021, Volume 37, Issue 41, 12027–12037, 37, 2021, Page(s) 37, 41, ISSN 0743-7463
Publisher: American Chemical Society
DOI: 10.1021/acs.langmuir.1c01660

Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation

Author(s): ANDREA RIDOLFI, LUCREZIA CASELLI, COSTANZA MONTIS, GAETANO MANGIAPIA, DEBORA BERTI, MARCO BRUCALE, FRANCESCO VALLE
Published in: Journal of Microscopy, 2020, ISSN 0022-2720
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/jmi.12910

Deterministic Lateral Displacement: Challenges and Perspectives

Author(s): Axel Hochstetter; Rohan Vernekar; Robert H. Austin; Holger Becker; Jason P. Beech; Dmitry A. Fedosov; Gerhard Gompper; Sungcheol Kim; Joshua T. Smith; Gustavo Stolovitzky; Jonas O. Tegenfeldt; Benjamin H. Wunsch; Kerwin Kwek Zeming; Timm Krüger; David W. Inglis
Published in: Hochstetter , A , Vernekar , R , Austin , R H , Becker , H , Beech , J P , Fedosov , D A , Gompper , G , Kim , S C , Smith , J T , Stolovitzky , G , Tegenfeldt , J O , Wunsch , B H , Zeming , K K , Krüger , T & Inglis , D W 2020 , ' Deterministic Lateral Displacement : Challenges and Perspectives ' , ACS Nano , vol. 14 , no. 9 , pp. 10784-10795 . https://doi.org/10.1021/acsnano.0c05186, 14, 2020, Page(s) 10784−10795, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.0c05186

Small extracellular vesicles from malignant ascites of patients with advanced ovarian cancer provide insights into the dynamics of the extracellular matrix.

Author(s): Bortot B, Apollonio M, Rampazzo E, Valle F, Brucale M, Ridolfi A, Ura B, Addobbati R, Di Lorenzo G, Romano F, Buonomo F, Ripepi C, Ricci G, Biffi S.
Published in: Mol Oncol. 2021 Dec;15(12), Mol Oncol. 2021 Dec;15(12), 2021, Page(s) 3596-3614., ISSN 1878-0261
Publisher: FEBS Press
DOI: 10.1002/1878-0261.13110

Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Author(s): Sara Busatto, George Vilanilam, Taylor Ticer, Wen-Lang Lin, Dennis Dickson, Shane Shapiro, Paolo Bergese, Joy Wolfram
Published in: Cells, 7/12, 2018, Page(s) 273, ISSN 2073-4409
Publisher: https://www.mdpi.com/
DOI: 10.3390/cells7120273

Active Posts in Deterministic Lateral Displacement Devices

Author(s): Jason P. Beech, Kevin Keim, Bao Dang Ho, Carlotta Guiducci, Jonas O. Tegenfeldt
Published in: Advanced Materials Technologies, 2019, Page(s) 1900339, ISSN 2365-709X
Publisher: https://onlinelibrary.wiley.com/
DOI: 10.1002/admt.201900339

The nanostructured secretome

Author(s): S. Busatto, A. Zendrini, A. Radeghieri, L. Paolini, M. Romano, M. Presta, P. Bergese
Published in: Biomaterials Science, 8/1, 2020, Page(s) 39-63, ISSN 2047-4830
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9bm01007f

Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop

Author(s): Ashley E. Russell, Alexandra Sneider, Kenneth W. Witwer, Paolo Bergese, Suvendra N. Bhattacharyya, Alexander Cocks, Emanuele Cocucci, Uta Erdbrügger, Juan M. Falcon-Perez, David W. Freeman, Thomas M. Gallagher, Shuaishuai Hu, Yiyao Huang, Steven M. Jay, Shin-ichi Kano, Gregory Lavieu, Aleksandra Leszczynska, Alicia M. Llorente, Quan Lu, Vasiliki Mahairaki, Dillon C. Muth, Nicole Noren Hooten, Mat
Published in: Journal of Extracellular Vesicles, 8/1, 2019, Page(s) 1684862, ISSN 2001-3078
Publisher: Journal of Extravesicles
DOI: 10.1080/20013078.2019.1684862

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions

Author(s): Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
Published in: Frontiers in Bioengineering and Biotechnology, 7, 2020, ISSN 2296-4185
Publisher: Front. Bioeng. Biotechnol.
DOI: 10.3389/fbioe.2019.00452

Interaction of nanoparticles with lipid films: the role of symmetry and shape anisotropy

Author(s): Lucrezia Caselli; Andrea Ridolfi; Gaetano Mangiapia; Pierfrancesco Maltoni; Jean-François Moulin; Debora Berti; Nina-Juliane Steinke; Emil Gustafsson; Tommy Nylander; Costanza Montis
Published in: Physical Chemistry Chemical Physics (PCCP), 2, 2021, ISSN 1463-9084
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1cp03201a

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Author(s): Ura B, Biffi S, Monasta L, Arrigoni G, Battisti I, Di Lorenzo G, Romano F, Aloisio M, Celsi F, Addobbati R, Valle F, Rampazzo E, Brucale M, Ridolfi A, Licastro D, Ricci G.
Published in: Cancers, 13(14), 2021, Page(s) 3639, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin

Author(s): Lucia Paolini, Stefania Federici, Giovanni Consoli, Diletta Arceri, Annalisa Radeghieri, Ivano Alessandri, Paolo Bergese
Published in: Journal of Extracellular Vesicles, 9/1, 2020, Page(s) 1741174, ISSN 2001-3078
Publisher: Journal of Extravesicles
DOI: 10.1080/20013078.2020.1741174

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis

Author(s): Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Published in: Cells, 10/3, 2021, Page(s) 544, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells10030544

Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses

Author(s): Marijke I. Zonneveld, Martijn J.C. Herwijnen, Marcela M. Fernandez‐Gutierrez, Alberta Giovanazzi, Anne Marit Groot, Marije Kleinjan, Toni M.M. Capel, Alice J.A.M. Sijts, Leonie S. Taams, Johan Garssen, Esther C. Jong, Michiel Kleerebezem, Esther N.M. Nolte‐’t Hoen, Frank A. Redegeld, Marca H.M. Wauben
Published in: Journal of Extracellular Vesicles, 10/5, 2021, ISSN 2001-3078
Publisher: Taylor and Francis Ltd.
DOI: 10.1002/jev2.12071

Charge-Based Separation of Micro- and Nanoparticles

Author(s): Bao Ho, Jason Beech, Jonas Tegenfeldt
Published in: Micromachines, 11/11, 2020, Page(s) 1014, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi11111014

Nanoanalytical analysis of bisphosphonate-driven alterations of microcalcifications using a 3D hydrogel system and in vivo mouse model

Author(s): Jessica L. Ruiz; Joshua D. Hutcheson; Luis Cardoso; Amirala Bakhshian Nik; Alexandra Condado de Abreu; Tan Pham; Fabrizio Buffolo; Sara Busatto; Stefania Federici; Andrea Ridolfi; Masanori Aikawa; Sergio Bertazzo; Paolo Bergese; Sheldon Weinbaum; Elena Aikawa
Published in: Proceedings of the National Academy of Sciences of the United States of America, Vol.118 No.14 e1811725118, 2021, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1811725118

Shedding light on membrane-templated clustering of gold nanoparticles

Author(s): Costanza Montis, Lucrezia Caselli, Francesco Valle, Andrea Zendrini, Francesco Carlà, Ralf Schweins, Marco Maccarini, Paolo Bergese, Debora Berti
Published in: Journal of Colloid and Interface Science, 573, 2020, Page(s) 204-214, ISSN 0021-9797
Publisher: Academic Press
DOI: 10.1016/j.jcis.2020.03.123