The Extracellular Vesicle Foundry

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Rezultaty

Dissemination and communication plan

Dissemination and communication plan prepared

Optimal storage and shipment of nanosized EVs

Definition and report on optimal storage and shipment of nanosized EVs

SOPs for quality standard nanosized EVs from bovine milk

Standard Operating Procedures (SOPs) for quality standard nanosized EVs from bovine milk defined.

Physical properties of EVs from the prototype benchmarked. Off-line testing.

Physiscochemical properties of EVs produced by the prototype benchmarked Offline testing

Colloidal characterization of nanosized EVs

Report on colloidal characterization of nanosized EVs

EV-surface interaction fundamentals

Report on EVsurface interaction fundamentals

First yearly dissemination and communication report

First annual dissemination and communication report

SOPs for quality standard nanosized EVs from Ascaris media

Standard Operating Procedures (SOPs) for quality standard nanosized EVs from Ascaris media defined.

Modulatory properties of EVs from the prototype benchmarked

Modulatory potential of EVs produced by the prototype benchmarked

Second yearly dissemination and communication report
Feasibility of large scale manufacturing of immune modulatory EVs

Report on feasibility including performance of large scale manufacturing immune modulatory EVs

First public report published

Final public report on the project progress.

Organization of a dedicated summer/winter school

Organisation of a dedicated summer/winter school on the project interdisciplinary themes. Teachers will be both from the consortium and external. Open to undergraduate and PHD students.

Final public report published

Final public report on the project progress

Library of nano-DLD surfaces

Library of nano-DLD surfaces fabricated.

Library of nanostructured surfaces non-lamellar lipid phases

Library of nanostructured surfaces made of non-lamellar lipid phases fabricated.

Large-scale production prototype assembled
Library of nanostructured surfaces classified by EV interactions

Library of relevant nanostructured surfaces classified in terms of their interactions with the EVs

Integrated modular system for production of EVs

Proof-of-concept integrated modular system assembled

Internal and main external communication channels

Internal and main external communication channels defined and operating

Project website online and logo

Project website online and logo defined.

Publikacje

Comparison of separation methods for immunomodulatory extracellular vesicles from helminths.

Autorzy: Borup,A.,Boysen,A.T.,Ridolfi,A.,Brucale,M.,Valle,F.,Paolini,L.,Bergese,P.,&Nejsum,P.
Opublikowane w: Journal of Extracellular Biology, Numer 2022;1:e41, 2022, Strona(/y) 1-17, ISSN 2768-2811
Wydawca: John Wiley & Sons, Ltd
DOI: 10.1002/jex2.41

Nanoanalytical analysis of bisphosphonate-driven alterations of microcalcifications using a 3D hydrogel system and in vivo mouse model

Autorzy: Ruiz JL, Hutcheson JD, Cardoso L, Bakhshian Nik A, Condado de Abreu A, Pham T, Buffolo F, Busatto S, Federici S, Ridolfi A, Aikawa M, Bertazzo S, Bergese P, Weinbaum S, Aikawa E.
Opublikowane w: Proceedings of the National Academy of Sciences, Numer 118 (14), 2021, Strona(/y) 1811725118, ISSN 0027-8424
Wydawca: National Academy of Sciences
DOI: 10.1073/pnas.1811725118

Nanoparticles and organized lipid assemblies: from interaction to design of hybrid soft devices

Autorzy: Marco Mendozza, Lucrezia Caselli, Annalisa Salvatore, Costanza Montis, Debora Berti
Opublikowane w: Soft Matter, Numer 15/44, 2019, Strona(/y) 8951-8970, ISSN 1744-683X
Wydawca: Royal Society of Chemistry
DOI: 10.1039/c9sm01601e

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Autorzy: Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Opublikowane w: Nanoscale Horizons, Numer 6/7, 2021, Strona(/y) 543-550, ISSN 2055-6756
Wydawca: The Royal Society of Chemistry 2021
DOI: 10.1039/d1nh00012h

Deterministic Lateral Displacement: Challenges and Perspectives.

Autorzy: Hochstetter A, Vernekar R, Austin RH, Becker H, Beech JP, Fedosov DA, Gompper G, Kim SC, Smith JT, Stolovitzky G, Tegenfeldt JO, Wunsch BH, Zeming KK, Krüger T, Inglis DW.
Opublikowane w: ACS Nano, Numer 14(9), 2020, Strona(/y) 10784-10795, ISSN 1936-0851
Wydawca: American Chemical Society
DOI: 10.1021/acsnano.0c05186

Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces

Autorzy: Costanza Montis, Annalisa Salvatore, Francesco Valle, Lucia Paolini, Francesco Carlà, Paolo Bergese, Debora Berti
Opublikowane w: Journal of Colloid and Interface Science, Numer 570, 2020, Strona(/y) 340-349, ISSN 0021-9797
Wydawca: Academic Press
DOI: 10.1016/j.jcis.2020.03.014

AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles

Autorzy: Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T. Boysen, Francesca Loria, Martijn J. C. van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H. M. Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Opublikowane w: Analytical Chemistry, Numer 92/15, 2020, Strona(/y) 10274-10282, ISSN 0003-2700
Wydawca: American Chemical Society
DOI: 10.1021/acs.analchem.9b05716

Fluorescent Labeling of Helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach

Autorzy: Anders T. Boysen, Bradley Whitehead, Allan Stensballe, Anna Carnerup, Tommy Nylander, Peter Nejsum
Opublikowane w: Biomedicines, Numer 8/7, 2020, Strona(/y) 213, ISSN 2227-9059
Wydawca: Biomedicines
DOI: 10.3390/biomedicines8070213

Regular Industrial Processing of Bovine Milk Impacts the Integrity and Molecular Composition of Extracellular Vesicles

Autorzy: Marije Kleinjan, Martijn JC van Herwijnen, Sten FWM Libregts, RJ Joost van Neerven, Anouk L Feitsma, Marca HM Wauben
Opublikowane w: The Journal of Nutrition, Numer 151/6, 2021, Strona(/y) 1416-1425, ISSN 0022-3166
Wydawca: American Society for Nutritional Sciences
DOI: 10.1093/jn/nxab031

Cell Sorting Using Electrokinetic Deterministic Lateral Displacement

Autorzy: Ho BD, Beech JP, Tegenfeldt JO
Opublikowane w: Micromachines, Numer 12(1), 2020, Strona(/y) 30, ISSN 2072-666X
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010030

Electrokinetic deterministic lateral displacement for fractionation of vesicles and nano-particles

Autorzy: Richard J. Gillams, Victor Calero, Raul Fernandez-Mateo and Hywel Morgan
Opublikowane w: Lab on a chip, Numer 14730189, 2022, Strona(/y) 3869-3876, ISSN 1473-0189
Wydawca: Royal Society of Chemistry
DOI: 10.1039/d2lc00583b

Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation

Autorzy: ANDREA RIDOLFI, LUCREZIA CASELLI, COSTANZA MONTIS, GAETANO MANGIAPIA, DEBORA BERTI, MARCO BRUCALE, FRANCESCO VALLE
Opublikowane w: Journal of Microscopy, 2020, ISSN 0022-2720
Wydawca: Blackwell Publishing Inc.
DOI: 10.1111/jmi.12910

Small extracellular vesicles from malignant ascites of patients with advanced ovarian cancer provide insights into the dynamics of the extracellular matrix.

Autorzy: Bortot B, Apollonio M, Rampazzo E, Valle F, Brucale M, Ridolfi A, Ura B, Addobbati R, Di Lorenzo G, Romano F, Buonomo F, Ripepi C, Ricci G, Biffi S.
Opublikowane w: Mol Oncol. 2021 Dec;15(12), Numer Mol Oncol. 2021 Dec;15(12), 2021, Strona(/y) 3596-3614., ISSN 1878-0261
Wydawca: FEBS Press
DOI: 10.1002/1878-0261.13110

Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Autorzy: Sara Busatto, George Vilanilam, Taylor Ticer, Wen-Lang Lin, Dennis Dickson, Shane Shapiro, Paolo Bergese, Joy Wolfram
Opublikowane w: Cells, Numer 7/12, 2018, Strona(/y) 273, ISSN 2073-4409
Wydawca: https://www.mdpi.com/
DOI: 10.3390/cells7120273

Active Posts in Deterministic Lateral Displacement Devices

Autorzy: Jason P. Beech, Kevin Keim, Bao Dang Ho, Carlotta Guiducci, Jonas O. Tegenfeldt
Opublikowane w: Advanced Materials Technologies, 2019, Strona(/y) 1900339, ISSN 2365-709X
Wydawca: https://onlinelibrary.wiley.com/
DOI: 10.1002/admt.201900339

The nanostructured secretome

Autorzy: S. Busatto, A. Zendrini, A. Radeghieri, L. Paolini, M. Romano, M. Presta, P. Bergese
Opublikowane w: Biomaterials Science, Numer 8/1, 2020, Strona(/y) 39-63, ISSN 2047-4830
Wydawca: Royal Society of Chemistry
DOI: 10.1039/c9bm01007f

Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop

Autorzy: Ashley E. Russell, Alexandra Sneider, Kenneth W. Witwer, Paolo Bergese, Suvendra N. Bhattacharyya, Alexander Cocks, Emanuele Cocucci, Uta Erdbrügger, Juan M. Falcon-Perez, David W. Freeman, Thomas M. Gallagher, Shuaishuai Hu, Yiyao Huang, Steven M. Jay, Shin-ichi Kano, Gregory Lavieu, Aleksandra Leszczynska, Alicia M. Llorente, Quan Lu, Vasiliki Mahairaki, Dillon C. Muth, Nicole Noren Hooten, Mat
Opublikowane w: Journal of Extracellular Vesicles, Numer 8/1, 2019, Strona(/y) 1684862, ISSN 2001-3078
Wydawca: Journal of Extravesicles
DOI: 10.1080/20013078.2019.1684862

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions

Autorzy: Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
Opublikowane w: Frontiers in Bioengineering and Biotechnology, Numer 7, 2020, ISSN 2296-4185
Wydawca: Front. Bioeng. Biotechnol.
DOI: 10.3389/fbioe.2019.00452

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Autorzy: Ura B, Biffi S, Monasta L, Arrigoni G, Battisti I, Di Lorenzo G, Romano F, Aloisio M, Celsi F, Addobbati R, Valle F, Rampazzo E, Brucale M, Ridolfi A, Licastro D, Ricci G.
Opublikowane w: Cancers, Numer 13(14), 2021, Strona(/y) 3639, ISSN 2072-6694
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin

Autorzy: Lucia Paolini, Stefania Federici, Giovanni Consoli, Diletta Arceri, Annalisa Radeghieri, Ivano Alessandri, Paolo Bergese
Opublikowane w: Journal of Extracellular Vesicles, Numer 9/1, 2020, Strona(/y) 1741174, ISSN 2001-3078
Wydawca: Journal of Extravesicles
DOI: 10.1080/20013078.2020.1741174

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis

Autorzy: Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Opublikowane w: Cells, Numer 10/3, 2021, Strona(/y) 544, ISSN 2073-4409
Wydawca: MDPI
DOI: 10.3390/cells10030544

Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses

Autorzy: Marijke I. Zonneveld, Martijn J.C. Herwijnen, Marcela M. Fernandez‐Gutierrez, Alberta Giovanazzi, Anne Marit Groot, Marije Kleinjan, Toni M.M. Capel, Alice J.A.M. Sijts, Leonie S. Taams, Johan Garssen, Esther C. Jong, Michiel Kleerebezem, Esther N.M. Nolte‐’t Hoen, Frank A. Redegeld, Marca H.M. Wauben
Opublikowane w: Journal of Extracellular Vesicles, Numer 10/5, 2021, ISSN 2001-3078
Wydawca: Taylor and Francis Ltd.
DOI: 10.1002/jev2.12071

Human milk extracellular vesicles preserve bronchial epithelialbarrier integrity and reduce TLR3-induced inflammation in vitro

Autorzy: Nikita Karra; Martijn J. C. Van Herwijnen; Marca H. M. Wauben; Emily Jane Swindle; Hywel Morgan
Opublikowane w: Journal of Extracellular Biology, Numer 20013078, 2022, Strona(/y) e56, ISSN 2001-3078
Wydawca: Wiley
DOI: 10.1002/jex2.54

Charge-Based Separation of Micro- and Nanoparticles

Autorzy: Bao Ho, Jason Beech, Jonas Tegenfeldt
Opublikowane w: Micromachines, Numer 11/11, 2020, Strona(/y) 1014, ISSN 2072-666X
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi11111014

Shedding light on membrane-templated clustering of gold nanoparticles

Autorzy: Costanza Montis, Lucrezia Caselli, Francesco Valle, Andrea Zendrini, Francesco Carlà, Ralf Schweins, Marco Maccarini, Paolo Bergese, Debora Berti
Opublikowane w: Journal of Colloid and Interface Science, Numer 573, 2020, Strona(/y) 204-214, ISSN 0021-9797
Wydawca: Academic Press
DOI: 10.1016/j.jcis.2020.03.123

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