Hyperpolarized Nuclear Magnetic Resonance Spectroscopy for Time-Resolved Monitoring of Interactions of Intrinsically Disordered Breast-Cancer Proteins

Project Information

HYPROTIN

Grant agreement ID: 801936

DOI

10.3030/801936

Project closed

EC signature date 16 January 2019

Start date 1 March 2019

End date 31 August 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 990 728,00

EU contribution

€ 1 990 728,00

Coordinated by

UNIVERSITAT WIEN
Austria

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Open Research Data Pilot (1)

Data Management Plan

Data Management Plan - ORDP: Open Research Data Pilot.

Publications

Peer reviewed articles (32)

Understanding Self-Assembly of Silica-Precipitating Peptides to Control Silica Particle Morphology

Author(s): Johannes Strobl, Fanny Kozak, Meder Kamalov, Daniela Reichinger, Dennis Kurzbach, Christian FW Becker
Published in: Adv. Mater., 2023, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202207586

Evaporation‐Induced Self‐Assembly of Small Peptide‐Conjugated Silica Nanoparticles

Author(s): Cornelia Baeckmann, Guilherme M. D. M. Rubio, Hanspeter Kählig, Dennis Kurzbach, Michael R. Reithofer, Freddy Kleitz
Published in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202108378

A Switch between Two Intrinsically Disordered Conformational Ensembles Modulates the Active Site of a Basic-Helix–Loop–Helix Transcription Factor

Author(s): Giuseppe Sicoli, Thomas Kress, Hervé Vezin, Karin Ledolter, Dennis Kurzbach
Published in: The Journal of Physical Chemistry Letters, 2020, Page(s) 8944-8951, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.0c02242

Identification of the Allosteric Binding Site for Thiazolopyrimidine on the C-Type Lectin Langerin

Author(s): Zhang, Hengxi; Modenutti, Carlos; Nekkanti, Yelha Phani Kumar; Denis, Maxime; Bermejo, Iris A.; Lefèbre, Jonathan; Che, Kateryna; Kim, Dongyoon; Kagelmacher, Marten; Kurzbach, Dennis; Nazaré, Marc; Rademacher, Christoph; 0000-0001-8864-4852; 0000-0001-6455-2136; 0000-0002-1602-2330; 0000-0001-7082-7239
Published in: ACS Chemical Biology, 2022, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.17169/refubium-37426

Frémy’s Salt as a Low-Persistence Hyperpolarization Agent: Efficient Dynamic Nuclear Polarization Plus Rapid Radical Scavenging

Author(s): Mattia Negroni; Ertan Turhan; Thomas Kress; Morgan Ceillier; Sami Jannin; Dennis Kurzbach
Published in: J. Am. Chem. Soc., 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c07960

Hyperpolarized nuclear Overhauser enhancement of alanine methyl groups by doubly relayed proton exchange

Author(s): Milan Zachrdla, Ertan Turhan, Christopher Pötzl, Aude Sadet, Paul R. Vasos, Dennis Kurzbach
Published in: Journal of Magnetic Resonance, Issue 364, 2024, Page(s) 107727, ISSN 1090-7807
Publisher: Academic Press
DOI: 10.1016/j.jmr.2024.107727

1H, 13C and 15N backbone resonance assignment of BRCA1 fragment 219–504

Author(s): Michał Górka, Szymon Żerko, Robert Konrat, Wiktor Koźmiński, Dennis Kurzbach
Published in: Biomolecular NMR Assignments, Issue 14/2, 2020, Page(s) 289-293, ISSN 1874-2718
Publisher: Springer Verlag
DOI: 10.1007/s12104-020-09963-6

Nuclear Overhauser spectroscopy in hyperpolarized water – chemical vs. magnetic exchange

Author(s): Ludovica Martina Epasto; Philipp Honegger; Kateryna Che; Fanny Kozak; Florian Jörg; Christian Schröder; Dennis Kurzbach
Published in: Chem Comm., 2022, ISSN 1364-548X
Publisher: RSC
DOI: 10.1039/d2cc03735a

A novel sample handling system for dissolution dynamic nuclear polarization experiments

Author(s): Thomas Kress, Kateryna Che, Ludovica M. Epasto, Fanny Kozak, Mattia Negroni, Gregory L. Olsen, Albina Selimovic, Dennis Kurzbach
Published in: Magnetic Resonance, Issue 2/1, 2021, Page(s) 387-394, ISSN 2699-0016
Publisher: Copernicus
DOI: 10.5194/mr-2-387-2021

Residue-resolved monitoring of protein hyperpolarization at sub-second time resolution

Author(s): Mattia Negroni, Dennis Kurzbach
Published in: Communications Chemistry, 2021, ISSN 2399-3669
Publisher: Springer Nature
DOI: 10.1038/s42004-021-00587-y

An Atomistic View on the Mechanism of Diatom Peptide‐Guided Biomimetic Silica Formation

Author(s): Fanny Kozak, Dörte Brandis, Christopher Pötzl, Ludovica M. Epasto, Daniela Reichinger, Dominik Obrist, Herwig Peterlik, Anton Polyansky, Bojan Zagrovic, Fabian Daus, Armin Geyer, Christian FW Becker, Dennis Kurzbach
Published in: Advanced Science, Issue 11, 2024, ISSN 2198-3844
Publisher: Wiley-VCH
DOI: 10.1002/advs.202401239

Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization

Author(s): Ertan Turhan, Masoud Minaei, Pooja Narwal, Benno Meier, Karel Kouřil, Dennis Kurzbach
Published in: Communications Chemistry, Issue 7, 2024, ISSN 2399-3669
Publisher: Springer
DOI: 10.1038/s42004-024-01300-5

Biphasic NMR of Hyperpolarized Suspensions─Real-Time Monitoring of Solute-to-Solid Conversion to Watch Materials Grow

Author(s): Ertan Turhan, Christopher Pötzl, Waldemar Keil, Mattia Negroni, Karel Kouřil, Benno Meier, Javier Agustin Romero, Krzysztof Kazimierczuk, Ieva Goldberga, Thierry Azaïs, Dennis Kurzbach
Published in: J. Phys. Chem. C, 2023, ISSN 1932-7447
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcc.3c04198

Ingredient-Dependent Extent of Lipid Oxidation in Margarine

Author(s): Sarah Fruehwirth, Sandra Egger, Dennis Kurzbach, Jakob Windisch, Franz Jirsa, Thomas Flecker, Miriam Ressler, Agnes T. Reiner, Nesrin Firat, Marc Pignitter
Published in: Antioxidants, Issue 10/1, 2021, Page(s) 105, ISSN 2076-3921
Publisher: MDPI
DOI: 10.3390/antiox10010105

Assessing the Onset of Calcium Phosphate Nucleation by Hyperpolarized Real-Time NMR

Author(s): Emmanuelle M. M. Weber, Thomas Kress, Daniel Abergel, Steffi Sewsurn, Thierry Azaïs, Dennis Kurzbach
Published in: Analytical Chemistry, Issue 92/11, 2020, Page(s) 7666-7673, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.0c00516

Formation and Evolution of Nanoscale Calcium Phosphate Precursors under Biomimetic Conditions

Author(s): Ludovica M. Epasto; Tristan Georges; Albina Selimović; Jean-Michel Guigner; Thierry Azaïs; Dennis Kurzbach
Published in: Analytical Chemistry, Issue 4, 2021, ISSN 1520-6882
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.1c01561

Missing Pieces in Structure Puzzles: How Hyperpolarized NMR Spectroscopy Can Complement Structural Biology and Biochemistry

Author(s): Mattia Negroni, Dennis Kurzbach
Published in: ChemBioChem, 2023, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200703

Inversion of Hyperpolarized ¹³C NMR Signals through Cross-Correlated Cross-Relaxation in Dissolution DNP Experiments

Author(s): Mattia Negroni; David Guarin; Kateryna Che; Ludovica M. Epasto; Ertan Turhan; Albina Selimović; Fanny Kozak; Samuel Cousin; Daniel Abergel; Geoffrey Bodenhausen; Dennis Kurzbach
Published in: J. Phys. Chem. B, Issue 3, 2022, ISSN 1520-6106
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.2c03375

Coupled MD simulations and NMR reveal that the intrinsically disordered domain of the breast-cancer susceptibility 1 protein (BRCA1) binds head-on to DNA double-strand ends

Author(s): Kateryna Che, Thomas Kress, Michał Górka, Szymon Żerko, Wiktor Kozminski, Dennis Kurzbach
Published in: J. Magn. Reson. Open, 2022, ISSN 2666-4410
Publisher: Elsevier
DOI: 10.1016/j.jmro.2022.100069

Long-Lived States in Hyperpolarized Deuterated Methyl Groups Reveal Weak Binding of Small Molecules to Proteins

Author(s): Thomas Kress, Astrid Walrant, Geoffrey Bodenhausen, Dennis Kurzbach
Published in: The Journal of Physical Chemistry Letters, 2019, Page(s) 1523-1529, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.9b00149

Conformational tuning of a DNA-bound transcription factor

Author(s): Giuseppe Sicoli, Hervé Vezin, Karin Ledolter, Thomas Kress, Dennis Kurzbach
Published in: Nucleic Acids Research, Issue 47/10, 2019, Page(s) 5429-5435, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkz291

Application and methodology of dissolution dynamic nuclear polarization in physical, chemical and biological contexts

Author(s): Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau, Dennis Kurzbach
Published in: Journal of Magnetic Resonance, Issue 305, 2019, Page(s) 41-50, ISSN 1090-7807
Publisher: Academic Press
DOI: 10.1016/j.jmr.2019.06.001

Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water

Author(s): Gregory L. Olsen, Or Szekely, Borja Mateos, Pavel Kadeřávek, Fabien Ferrage, Robert Konrat, Roberta Pierattelli, Isabella C. Felli, Geoffrey Bodenhausen, Dennis Kurzbach, Lucio Frydman
Published in: Journal of Biomolecular NMR, Issue 74/2-3, 2020, Page(s) 161-171, ISSN 0925-2738
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10858-020-00301-5

Nature-inspired dimerization as a strategy to modulate neuropeptide pharmacology exemplified with vasopressin and oxytocin

Author(s): Zoltan Dekan, Thomas Kremsmayr, Peter Keov, Mathilde Godin, Ngari Teakle, Leopold Dürrauer, Huang Xiang, Dalia Gharib, Christian Bergmayr, Roland Hellinger, Marina Gay, Marta Vilaseca, Dennis Kurzbach, Fernando Albericio, Paul F. Alewood, Christian W. Gruber, Markus Muttenthaler
Published in: Chemical Science, Issue 12/11, 2021, Page(s) 4057-4062, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc05501h

Branched Polymeric Prenucleation Assemblies Initiate Calcium Phosphate Precipitation

Author(s): Ertan Turhan, Ieva Goldberga, Christopher Pötzl, Waldemar Keil, Jean-Michel Guigner, Martin F.T. Haßler, Herwig Peterlik, Thierry Azaïs, Dennis Kurzbach
Published in: Journal of the American Chemical Society, Issue 146, 2024, Page(s) 25614-25624, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.4c07325

Hyperpolarized NMR Metabolomics at Natural 13 C Abundance

Author(s): Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau
Published in: Analytical Chemistry, Issue 92/22, 2020, Page(s) 14867-14871, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.0c03510

Unified understanding of the breakdown of thermal mixing dynamic nuclear polarization: The role of temperature and radical concentration

Author(s): Ludovica M. Epasto, Thibaud Maimbourg, Alberto Rosso, Dennis Kurzbach
Published in: Journal of Magnetic Resonance, Issue 362, 2024, Page(s) 107670, ISSN 1090-7807
Publisher: Academic Press
DOI: 10.1016/j.jmr.2024.107670

How to assess the structural dynamics of transcription factors by integrating sparse NMR and EPR constraints with molecular dynamics simulations

Author(s): Fanny Kozak, Dennis Kurzbach
Published in: Computational and Structural Biotechnology Journal, Issue 19, 2021, Page(s) 2097-2105, ISSN 2001-0370
Publisher: Elsevier
DOI: 10.1016/j.csbj.2021.04.020

<scp>NMR</scp>‐identification of the interaction between <scp>BRCA1</scp> and the intrinsically disordered monomer of the Myc‐associated factor X

Author(s): Ludovica Martina Epasto, Christopher Pötzl, Herwig Peterlik, Mahdi Khalil, Christine Saint‐Pierre, Didier Gasparutto, Giuseppe Sicoli, Dennis Kurzbach
Published in: Protein Science, Issue 33, 2024, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4849

Conformational selection of vasopressin upon V1a receptor binding.

Author(s): Kateryna Che; Markus Muttenthaler; Dennis Kurzbach
Published in: Computational and Structural Biotechnology Journal, Vol 19, Issue 9, 2021, Page(s) 5826-5833, ISSN 2001-0370
Publisher: Elsevier
DOI: 10.1016/j.csbj.2021.10.024

Constraining and Modifying Peptides Using Pd-Mediated Cysteine Allylation

Author(s): Julia Kriegesmann, Thomas Schlatzer, Kateryna Che, Claudia Altdorf, Susanne Huhmann, Hanspeter Kählig, Dennis Kurzbach, Rolf Breinbauer, Christian F. W. Becker
Published in: ChemBioChem, 2023, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300098

Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces

Author(s): Ludovica Martina Epasto, Kateryna Che, Fanny Kozak, Albina Selimovic, Pavel Kaderavek, Dennis Kurzbach
Published in: Science Advances, 2022, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.abq5179

Non-peer reviewed articles (1)

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Author(s): Christian Hilty, Dennis Kurzbach, Lucio Frydman
Published in: Nature Protocols, 2022, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-022-00693-8