Hyperpolarized Nuclear Magnetic Resonance Spectroscopy for Time-Resolved Monitoring of Interactions of Intrinsically Disordered Breast-Cancer Proteins

Informazioni relative al progetto

HYPROTIN

ID dell’accordo di sovvenzione: 801936

DOI

10.3030/801936

Progetto chiuso

Data della firma CE 16 Gennaio 2019

Data di avvio 1 Marzo 2019

Data di completamento 31 Agosto 2024

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 990 728,00

Contributo UE

€ 1 990 728,00

Coordinato da

UNIVERSITAT WIEN
Austria

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Open Research Data Pilot (1)

Data Management Plan

Data Management Plan - ORDP: Open Research Data Pilot.

Pubblicazioni

Peer reviewed articles (32)

Understanding Self-Assembly of Silica-Precipitating Peptides to Control Silica Particle Morphology

Autori: Johannes Strobl, Fanny Kozak, Meder Kamalov, Daniela Reichinger, Dennis Kurzbach, Christian FW Becker
Pubblicato in: Adv. Mater., 2023, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202207586

Evaporation‐Induced Self‐Assembly of Small Peptide‐Conjugated Silica Nanoparticles

Autori: Cornelia Baeckmann, Guilherme M. D. M. Rubio, Hanspeter Kählig, Dennis Kurzbach, Michael R. Reithofer, Freddy Kleitz
Pubblicato in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202108378

A Switch between Two Intrinsically Disordered Conformational Ensembles Modulates the Active Site of a Basic-Helix–Loop–Helix Transcription Factor

Autori: Giuseppe Sicoli, Thomas Kress, Hervé Vezin, Karin Ledolter, Dennis Kurzbach
Pubblicato in: The Journal of Physical Chemistry Letters, 2020, Pagina/e 8944-8951, ISSN 1948-7185
Editore: American Chemical Society
DOI: 10.1021/acs.jpclett.0c02242

Identification of the Allosteric Binding Site for Thiazolopyrimidine on the C-Type Lectin Langerin

Autori: Zhang, Hengxi; Modenutti, Carlos; Nekkanti, Yelha Phani Kumar; Denis, Maxime; Bermejo, Iris A.; Lefèbre, Jonathan; Che, Kateryna; Kim, Dongyoon; Kagelmacher, Marten; Kurzbach, Dennis; Nazaré, Marc; Rademacher, Christoph; 0000-0001-8864-4852; 0000-0001-6455-2136; 0000-0002-1602-2330; 0000-0001-7082-7239
Pubblicato in: ACS Chemical Biology, 2022, ISSN 1554-8929
Editore: American Chemical Society
DOI: 10.17169/refubium-37426

Frémy’s Salt as a Low-Persistence Hyperpolarization Agent: Efficient Dynamic Nuclear Polarization Plus Rapid Radical Scavenging

Autori: Mattia Negroni; Ertan Turhan; Thomas Kress; Morgan Ceillier; Sami Jannin; Dennis Kurzbach
Pubblicato in: J. Am. Chem. Soc., 2022, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.2c07960

Hyperpolarized nuclear Overhauser enhancement of alanine methyl groups by doubly relayed proton exchange

Autori: Milan Zachrdla, Ertan Turhan, Christopher Pötzl, Aude Sadet, Paul R. Vasos, Dennis Kurzbach
Pubblicato in: Journal of Magnetic Resonance, Numero 364, 2024, Pagina/e 107727, ISSN 1090-7807
Editore: Academic Press
DOI: 10.1016/j.jmr.2024.107727

1H, 13C and 15N backbone resonance assignment of BRCA1 fragment 219–504

Autori: Michał Górka, Szymon Żerko, Robert Konrat, Wiktor Koźmiński, Dennis Kurzbach
Pubblicato in: Biomolecular NMR Assignments, Numero 14/2, 2020, Pagina/e 289-293, ISSN 1874-2718
Editore: Springer Verlag
DOI: 10.1007/s12104-020-09963-6

Nuclear Overhauser spectroscopy in hyperpolarized water – chemical vs. magnetic exchange

Autori: Ludovica Martina Epasto; Philipp Honegger; Kateryna Che; Fanny Kozak; Florian Jörg; Christian Schröder; Dennis Kurzbach
Pubblicato in: Chem Comm., 2022, ISSN 1364-548X
Editore: RSC
DOI: 10.1039/d2cc03735a

A novel sample handling system for dissolution dynamic nuclear polarization experiments

Autori: Thomas Kress, Kateryna Che, Ludovica M. Epasto, Fanny Kozak, Mattia Negroni, Gregory L. Olsen, Albina Selimovic, Dennis Kurzbach
Pubblicato in: Magnetic Resonance, Numero 2/1, 2021, Pagina/e 387-394, ISSN 2699-0016
Editore: Copernicus
DOI: 10.5194/mr-2-387-2021

Residue-resolved monitoring of protein hyperpolarization at sub-second time resolution

Autori: Mattia Negroni, Dennis Kurzbach
Pubblicato in: Communications Chemistry, 2021, ISSN 2399-3669
Editore: Springer Nature
DOI: 10.1038/s42004-021-00587-y

An Atomistic View on the Mechanism of Diatom Peptide‐Guided Biomimetic Silica Formation

Autori: Fanny Kozak, Dörte Brandis, Christopher Pötzl, Ludovica M. Epasto, Daniela Reichinger, Dominik Obrist, Herwig Peterlik, Anton Polyansky, Bojan Zagrovic, Fabian Daus, Armin Geyer, Christian FW Becker, Dennis Kurzbach
Pubblicato in: Advanced Science, Numero 11, 2024, ISSN 2198-3844
Editore: Wiley-VCH
DOI: 10.1002/advs.202401239

Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization

Autori: Ertan Turhan, Masoud Minaei, Pooja Narwal, Benno Meier, Karel Kouřil, Dennis Kurzbach
Pubblicato in: Communications Chemistry, Numero 7, 2024, ISSN 2399-3669
Editore: Springer
DOI: 10.1038/s42004-024-01300-5

Biphasic NMR of Hyperpolarized Suspensions─Real-Time Monitoring of Solute-to-Solid Conversion to Watch Materials Grow

Autori: Ertan Turhan, Christopher Pötzl, Waldemar Keil, Mattia Negroni, Karel Kouřil, Benno Meier, Javier Agustin Romero, Krzysztof Kazimierczuk, Ieva Goldberga, Thierry Azaïs, Dennis Kurzbach
Pubblicato in: J. Phys. Chem. C, 2023, ISSN 1932-7447
Editore: American Chemical Society
DOI: 10.1021/acs.jpcc.3c04198

Ingredient-Dependent Extent of Lipid Oxidation in Margarine

Autori: Sarah Fruehwirth, Sandra Egger, Dennis Kurzbach, Jakob Windisch, Franz Jirsa, Thomas Flecker, Miriam Ressler, Agnes T. Reiner, Nesrin Firat, Marc Pignitter
Pubblicato in: Antioxidants, Numero 10/1, 2021, Pagina/e 105, ISSN 2076-3921
Editore: MDPI
DOI: 10.3390/antiox10010105

Assessing the Onset of Calcium Phosphate Nucleation by Hyperpolarized Real-Time NMR

Autori: Emmanuelle M. M. Weber, Thomas Kress, Daniel Abergel, Steffi Sewsurn, Thierry Azaïs, Dennis Kurzbach
Pubblicato in: Analytical Chemistry, Numero 92/11, 2020, Pagina/e 7666-7673, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.0c00516

Formation and Evolution of Nanoscale Calcium Phosphate Precursors under Biomimetic Conditions

Autori: Ludovica M. Epasto; Tristan Georges; Albina Selimović; Jean-Michel Guigner; Thierry Azaïs; Dennis Kurzbach
Pubblicato in: Analytical Chemistry, Numero 4, 2021, ISSN 1520-6882
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.1c01561

Missing Pieces in Structure Puzzles: How Hyperpolarized NMR Spectroscopy Can Complement Structural Biology and Biochemistry

Autori: Mattia Negroni, Dennis Kurzbach
Pubblicato in: ChemBioChem, 2023, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200703

Inversion of Hyperpolarized ¹³C NMR Signals through Cross-Correlated Cross-Relaxation in Dissolution DNP Experiments

Autori: Mattia Negroni; David Guarin; Kateryna Che; Ludovica M. Epasto; Ertan Turhan; Albina Selimović; Fanny Kozak; Samuel Cousin; Daniel Abergel; Geoffrey Bodenhausen; Dennis Kurzbach
Pubblicato in: J. Phys. Chem. B, Numero 3, 2022, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.2c03375

Coupled MD simulations and NMR reveal that the intrinsically disordered domain of the breast-cancer susceptibility 1 protein (BRCA1) binds head-on to DNA double-strand ends

Autori: Kateryna Che, Thomas Kress, Michał Górka, Szymon Żerko, Wiktor Kozminski, Dennis Kurzbach
Pubblicato in: J. Magn. Reson. Open, 2022, ISSN 2666-4410
Editore: Elsevier
DOI: 10.1016/j.jmro.2022.100069

Long-Lived States in Hyperpolarized Deuterated Methyl Groups Reveal Weak Binding of Small Molecules to Proteins

Autori: Thomas Kress, Astrid Walrant, Geoffrey Bodenhausen, Dennis Kurzbach
Pubblicato in: The Journal of Physical Chemistry Letters, 2019, Pagina/e 1523-1529, ISSN 1948-7185
Editore: American Chemical Society
DOI: 10.1021/acs.jpclett.9b00149

Conformational tuning of a DNA-bound transcription factor

Autori: Giuseppe Sicoli, Hervé Vezin, Karin Ledolter, Thomas Kress, Dennis Kurzbach
Pubblicato in: Nucleic Acids Research, Numero 47/10, 2019, Pagina/e 5429-5435, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkz291

Application and methodology of dissolution dynamic nuclear polarization in physical, chemical and biological contexts

Autori: Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau, Dennis Kurzbach
Pubblicato in: Journal of Magnetic Resonance, Numero 305, 2019, Pagina/e 41-50, ISSN 1090-7807
Editore: Academic Press
DOI: 10.1016/j.jmr.2019.06.001

Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water

Autori: Gregory L. Olsen, Or Szekely, Borja Mateos, Pavel Kadeřávek, Fabien Ferrage, Robert Konrat, Roberta Pierattelli, Isabella C. Felli, Geoffrey Bodenhausen, Dennis Kurzbach, Lucio Frydman
Pubblicato in: Journal of Biomolecular NMR, Numero 74/2-3, 2020, Pagina/e 161-171, ISSN 0925-2738
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10858-020-00301-5

Nature-inspired dimerization as a strategy to modulate neuropeptide pharmacology exemplified with vasopressin and oxytocin

Autori: Zoltan Dekan, Thomas Kremsmayr, Peter Keov, Mathilde Godin, Ngari Teakle, Leopold Dürrauer, Huang Xiang, Dalia Gharib, Christian Bergmayr, Roland Hellinger, Marina Gay, Marta Vilaseca, Dennis Kurzbach, Fernando Albericio, Paul F. Alewood, Christian W. Gruber, Markus Muttenthaler
Pubblicato in: Chemical Science, Numero 12/11, 2021, Pagina/e 4057-4062, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d0sc05501h

Branched Polymeric Prenucleation Assemblies Initiate Calcium Phosphate Precipitation

Autori: Ertan Turhan, Ieva Goldberga, Christopher Pötzl, Waldemar Keil, Jean-Michel Guigner, Martin F.T. Haßler, Herwig Peterlik, Thierry Azaïs, Dennis Kurzbach
Pubblicato in: Journal of the American Chemical Society, Numero 146, 2024, Pagina/e 25614-25624, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.4c07325

Hyperpolarized NMR Metabolomics at Natural 13 C Abundance

Autori: Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau
Pubblicato in: Analytical Chemistry, Numero 92/22, 2020, Pagina/e 14867-14871, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.0c03510

Unified understanding of the breakdown of thermal mixing dynamic nuclear polarization: The role of temperature and radical concentration

Autori: Ludovica M. Epasto, Thibaud Maimbourg, Alberto Rosso, Dennis Kurzbach
Pubblicato in: Journal of Magnetic Resonance, Numero 362, 2024, Pagina/e 107670, ISSN 1090-7807
Editore: Academic Press
DOI: 10.1016/j.jmr.2024.107670

How to assess the structural dynamics of transcription factors by integrating sparse NMR and EPR constraints with molecular dynamics simulations

Autori: Fanny Kozak, Dennis Kurzbach
Pubblicato in: Computational and Structural Biotechnology Journal, Numero 19, 2021, Pagina/e 2097-2105, ISSN 2001-0370
Editore: Elsevier
DOI: 10.1016/j.csbj.2021.04.020

<scp>NMR</scp>‐identification of the interaction between <scp>BRCA1</scp> and the intrinsically disordered monomer of the Myc‐associated factor X

Autori: Ludovica Martina Epasto, Christopher Pötzl, Herwig Peterlik, Mahdi Khalil, Christine Saint‐Pierre, Didier Gasparutto, Giuseppe Sicoli, Dennis Kurzbach
Pubblicato in: Protein Science, Numero 33, 2024, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4849

Conformational selection of vasopressin upon V1a receptor binding.

Autori: Kateryna Che; Markus Muttenthaler; Dennis Kurzbach
Pubblicato in: Computational and Structural Biotechnology Journal, Vol 19, Numero 9, 2021, Pagina/e 5826-5833, ISSN 2001-0370
Editore: Elsevier
DOI: 10.1016/j.csbj.2021.10.024

Constraining and Modifying Peptides Using Pd-Mediated Cysteine Allylation

Autori: Julia Kriegesmann, Thomas Schlatzer, Kateryna Che, Claudia Altdorf, Susanne Huhmann, Hanspeter Kählig, Dennis Kurzbach, Rolf Breinbauer, Christian F. W. Becker
Pubblicato in: ChemBioChem, 2023, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300098

Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces

Autori: Ludovica Martina Epasto, Kateryna Che, Fanny Kozak, Albina Selimovic, Pavel Kaderavek, Dennis Kurzbach
Pubblicato in: Science Advances, 2022, ISSN 2375-2548
Editore: AAAS
DOI: 10.1126/sciadv.abq5179

Non-peer reviewed articles (1)

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Autori: Christian Hilty, Dennis Kurzbach, Lucio Frydman
Pubblicato in: Nature Protocols, 2022, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/s41596-022-00693-8