Hyperpolarized Nuclear Magnetic Resonance Spectroscopy for Time-Resolved Monitoring of Interactions of Intrinsically Disordered Breast-Cancer Proteins

Información del proyecto

HYPROTIN

Identificador del acuerdo de subvención: 801936

DOI

10.3030/801936

Proyecto cerrado

Fecha de la firma de la CE 16 Enero 2019

Fecha de inicio 1 Marzo 2019

Fecha de finalización 31 Agosto 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 990 728,00

Aportación de la UE

€ 1 990 728,00

Coordinado por

UNIVERSITAT WIEN
Austria

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Open Research Data Pilot (1)

Data Management Plan

Data Management Plan - ORDP: Open Research Data Pilot.

Publicaciones

Peer reviewed articles (32)

Understanding Self-Assembly of Silica-Precipitating Peptides to Control Silica Particle Morphology

Autores: Johannes Strobl, Fanny Kozak, Meder Kamalov, Daniela Reichinger, Dennis Kurzbach, Christian FW Becker
Publicado en: Adv. Mater., 2023, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202207586

Evaporation‐Induced Self‐Assembly of Small Peptide‐Conjugated Silica Nanoparticles

Autores: Cornelia Baeckmann, Guilherme M. D. M. Rubio, Hanspeter Kählig, Dennis Kurzbach, Michael R. Reithofer, Freddy Kleitz
Publicado en: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202108378

A Switch between Two Intrinsically Disordered Conformational Ensembles Modulates the Active Site of a Basic-Helix–Loop–Helix Transcription Factor

Autores: Giuseppe Sicoli, Thomas Kress, Hervé Vezin, Karin Ledolter, Dennis Kurzbach
Publicado en: The Journal of Physical Chemistry Letters, 2020, Página(s) 8944-8951, ISSN 1948-7185
Editor: American Chemical Society
DOI: 10.1021/acs.jpclett.0c02242

Identification of the Allosteric Binding Site for Thiazolopyrimidine on the C-Type Lectin Langerin

Autores: Zhang, Hengxi; Modenutti, Carlos; Nekkanti, Yelha Phani Kumar; Denis, Maxime; Bermejo, Iris A.; Lefèbre, Jonathan; Che, Kateryna; Kim, Dongyoon; Kagelmacher, Marten; Kurzbach, Dennis; Nazaré, Marc; Rademacher, Christoph; 0000-0001-8864-4852; 0000-0001-6455-2136; 0000-0002-1602-2330; 0000-0001-7082-7239
Publicado en: ACS Chemical Biology, 2022, ISSN 1554-8929
Editor: American Chemical Society
DOI: 10.17169/refubium-37426

Frémy’s Salt as a Low-Persistence Hyperpolarization Agent: Efficient Dynamic Nuclear Polarization Plus Rapid Radical Scavenging

Autores: Mattia Negroni; Ertan Turhan; Thomas Kress; Morgan Ceillier; Sami Jannin; Dennis Kurzbach
Publicado en: J. Am. Chem. Soc., 2022, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.2c07960

Hyperpolarized nuclear Overhauser enhancement of alanine methyl groups by doubly relayed proton exchange

Autores: Milan Zachrdla, Ertan Turhan, Christopher Pötzl, Aude Sadet, Paul R. Vasos, Dennis Kurzbach
Publicado en: Journal of Magnetic Resonance, Edición 364, 2024, Página(s) 107727, ISSN 1090-7807
Editor: Academic Press
DOI: 10.1016/j.jmr.2024.107727

1H, 13C and 15N backbone resonance assignment of BRCA1 fragment 219–504

Autores: Michał Górka, Szymon Żerko, Robert Konrat, Wiktor Koźmiński, Dennis Kurzbach
Publicado en: Biomolecular NMR Assignments, Edición 14/2, 2020, Página(s) 289-293, ISSN 1874-2718
Editor: Springer Verlag
DOI: 10.1007/s12104-020-09963-6

Nuclear Overhauser spectroscopy in hyperpolarized water – chemical vs. magnetic exchange

Autores: Ludovica Martina Epasto; Philipp Honegger; Kateryna Che; Fanny Kozak; Florian Jörg; Christian Schröder; Dennis Kurzbach
Publicado en: Chem Comm., 2022, ISSN 1364-548X
Editor: RSC
DOI: 10.1039/d2cc03735a

A novel sample handling system for dissolution dynamic nuclear polarization experiments

Autores: Thomas Kress, Kateryna Che, Ludovica M. Epasto, Fanny Kozak, Mattia Negroni, Gregory L. Olsen, Albina Selimovic, Dennis Kurzbach
Publicado en: Magnetic Resonance, Edición 2/1, 2021, Página(s) 387-394, ISSN 2699-0016
Editor: Copernicus
DOI: 10.5194/mr-2-387-2021

Residue-resolved monitoring of protein hyperpolarization at sub-second time resolution

Autores: Mattia Negroni, Dennis Kurzbach
Publicado en: Communications Chemistry, 2021, ISSN 2399-3669
Editor: Springer Nature
DOI: 10.1038/s42004-021-00587-y

An Atomistic View on the Mechanism of Diatom Peptide‐Guided Biomimetic Silica Formation

Autores: Fanny Kozak, Dörte Brandis, Christopher Pötzl, Ludovica M. Epasto, Daniela Reichinger, Dominik Obrist, Herwig Peterlik, Anton Polyansky, Bojan Zagrovic, Fabian Daus, Armin Geyer, Christian FW Becker, Dennis Kurzbach
Publicado en: Advanced Science, Edición 11, 2024, ISSN 2198-3844
Editor: Wiley-VCH
DOI: 10.1002/advs.202401239

Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization

Autores: Ertan Turhan, Masoud Minaei, Pooja Narwal, Benno Meier, Karel Kouřil, Dennis Kurzbach
Publicado en: Communications Chemistry, Edición 7, 2024, ISSN 2399-3669
Editor: Springer
DOI: 10.1038/s42004-024-01300-5

Biphasic NMR of Hyperpolarized Suspensions─Real-Time Monitoring of Solute-to-Solid Conversion to Watch Materials Grow

Autores: Ertan Turhan, Christopher Pötzl, Waldemar Keil, Mattia Negroni, Karel Kouřil, Benno Meier, Javier Agustin Romero, Krzysztof Kazimierczuk, Ieva Goldberga, Thierry Azaïs, Dennis Kurzbach
Publicado en: J. Phys. Chem. C, 2023, ISSN 1932-7447
Editor: American Chemical Society
DOI: 10.1021/acs.jpcc.3c04198

Ingredient-Dependent Extent of Lipid Oxidation in Margarine

Autores: Sarah Fruehwirth, Sandra Egger, Dennis Kurzbach, Jakob Windisch, Franz Jirsa, Thomas Flecker, Miriam Ressler, Agnes T. Reiner, Nesrin Firat, Marc Pignitter
Publicado en: Antioxidants, Edición 10/1, 2021, Página(s) 105, ISSN 2076-3921
Editor: MDPI
DOI: 10.3390/antiox10010105

Assessing the Onset of Calcium Phosphate Nucleation by Hyperpolarized Real-Time NMR

Autores: Emmanuelle M. M. Weber, Thomas Kress, Daniel Abergel, Steffi Sewsurn, Thierry Azaïs, Dennis Kurzbach
Publicado en: Analytical Chemistry, Edición 92/11, 2020, Página(s) 7666-7673, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.0c00516

Formation and Evolution of Nanoscale Calcium Phosphate Precursors under Biomimetic Conditions

Autores: Ludovica M. Epasto; Tristan Georges; Albina Selimović; Jean-Michel Guigner; Thierry Azaïs; Dennis Kurzbach
Publicado en: Analytical Chemistry, Edición 4, 2021, ISSN 1520-6882
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.1c01561

Missing Pieces in Structure Puzzles: How Hyperpolarized NMR Spectroscopy Can Complement Structural Biology and Biochemistry

Autores: Mattia Negroni, Dennis Kurzbach
Publicado en: ChemBioChem, 2023, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200703

Inversion of Hyperpolarized ¹³C NMR Signals through Cross-Correlated Cross-Relaxation in Dissolution DNP Experiments

Autores: Mattia Negroni; David Guarin; Kateryna Che; Ludovica M. Epasto; Ertan Turhan; Albina Selimović; Fanny Kozak; Samuel Cousin; Daniel Abergel; Geoffrey Bodenhausen; Dennis Kurzbach
Publicado en: J. Phys. Chem. B, Edición 3, 2022, ISSN 1520-6106
Editor: American Chemical Society
DOI: 10.1021/acs.jpcb.2c03375

Coupled MD simulations and NMR reveal that the intrinsically disordered domain of the breast-cancer susceptibility 1 protein (BRCA1) binds head-on to DNA double-strand ends

Autores: Kateryna Che, Thomas Kress, Michał Górka, Szymon Żerko, Wiktor Kozminski, Dennis Kurzbach
Publicado en: J. Magn. Reson. Open, 2022, ISSN 2666-4410
Editor: Elsevier
DOI: 10.1016/j.jmro.2022.100069

Long-Lived States in Hyperpolarized Deuterated Methyl Groups Reveal Weak Binding of Small Molecules to Proteins

Autores: Thomas Kress, Astrid Walrant, Geoffrey Bodenhausen, Dennis Kurzbach
Publicado en: The Journal of Physical Chemistry Letters, 2019, Página(s) 1523-1529, ISSN 1948-7185
Editor: American Chemical Society
DOI: 10.1021/acs.jpclett.9b00149

Conformational tuning of a DNA-bound transcription factor

Autores: Giuseppe Sicoli, Hervé Vezin, Karin Ledolter, Thomas Kress, Dennis Kurzbach
Publicado en: Nucleic Acids Research, Edición 47/10, 2019, Página(s) 5429-5435, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkz291

Application and methodology of dissolution dynamic nuclear polarization in physical, chemical and biological contexts

Autores: Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau, Dennis Kurzbach
Publicado en: Journal of Magnetic Resonance, Edición 305, 2019, Página(s) 41-50, ISSN 1090-7807
Editor: Academic Press
DOI: 10.1016/j.jmr.2019.06.001

Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water

Autores: Gregory L. Olsen, Or Szekely, Borja Mateos, Pavel Kadeřávek, Fabien Ferrage, Robert Konrat, Roberta Pierattelli, Isabella C. Felli, Geoffrey Bodenhausen, Dennis Kurzbach, Lucio Frydman
Publicado en: Journal of Biomolecular NMR, Edición 74/2-3, 2020, Página(s) 161-171, ISSN 0925-2738
Editor: Kluwer Academic Publishers
DOI: 10.1007/s10858-020-00301-5

Nature-inspired dimerization as a strategy to modulate neuropeptide pharmacology exemplified with vasopressin and oxytocin

Autores: Zoltan Dekan, Thomas Kremsmayr, Peter Keov, Mathilde Godin, Ngari Teakle, Leopold Dürrauer, Huang Xiang, Dalia Gharib, Christian Bergmayr, Roland Hellinger, Marina Gay, Marta Vilaseca, Dennis Kurzbach, Fernando Albericio, Paul F. Alewood, Christian W. Gruber, Markus Muttenthaler
Publicado en: Chemical Science, Edición 12/11, 2021, Página(s) 4057-4062, ISSN 2041-6520
Editor: Royal Society of Chemistry
DOI: 10.1039/d0sc05501h

Branched Polymeric Prenucleation Assemblies Initiate Calcium Phosphate Precipitation

Autores: Ertan Turhan, Ieva Goldberga, Christopher Pötzl, Waldemar Keil, Jean-Michel Guigner, Martin F.T. Haßler, Herwig Peterlik, Thierry Azaïs, Dennis Kurzbach
Publicado en: Journal of the American Chemical Society, Edición 146, 2024, Página(s) 25614-25624, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.4c07325

Hyperpolarized NMR Metabolomics at Natural 13 C Abundance

Autores: Arnab Dey, Benoît Charrier, Estelle Martineau, Catherine Deborde, Elodie Gandriau, Annick Moing, Daniel Jacob, Dmitry Eshchenko, Marc Schnell, Roberto Melzi, Dennis Kurzbach, Morgan Ceillier, Quentin Chappuis, Samuel F. Cousin, James G. Kempf, Sami Jannin, Jean-Nicolas Dumez, Patrick Giraudeau
Publicado en: Analytical Chemistry, Edición 92/22, 2020, Página(s) 14867-14871, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.0c03510

Unified understanding of the breakdown of thermal mixing dynamic nuclear polarization: The role of temperature and radical concentration

Autores: Ludovica M. Epasto, Thibaud Maimbourg, Alberto Rosso, Dennis Kurzbach
Publicado en: Journal of Magnetic Resonance, Edición 362, 2024, Página(s) 107670, ISSN 1090-7807
Editor: Academic Press
DOI: 10.1016/j.jmr.2024.107670

How to assess the structural dynamics of transcription factors by integrating sparse NMR and EPR constraints with molecular dynamics simulations

Autores: Fanny Kozak, Dennis Kurzbach
Publicado en: Computational and Structural Biotechnology Journal, Edición 19, 2021, Página(s) 2097-2105, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/j.csbj.2021.04.020

<scp>NMR</scp>‐identification of the interaction between <scp>BRCA1</scp> and the intrinsically disordered monomer of the Myc‐associated factor X

Autores: Ludovica Martina Epasto, Christopher Pötzl, Herwig Peterlik, Mahdi Khalil, Christine Saint‐Pierre, Didier Gasparutto, Giuseppe Sicoli, Dennis Kurzbach
Publicado en: Protein Science, Edición 33, 2024, ISSN 0961-8368
Editor: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4849

Conformational selection of vasopressin upon V1a receptor binding.

Autores: Kateryna Che; Markus Muttenthaler; Dennis Kurzbach
Publicado en: Computational and Structural Biotechnology Journal, Vol 19, Edición 9, 2021, Página(s) 5826-5833, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/j.csbj.2021.10.024

Constraining and Modifying Peptides Using Pd-Mediated Cysteine Allylation

Autores: Julia Kriegesmann, Thomas Schlatzer, Kateryna Che, Claudia Altdorf, Susanne Huhmann, Hanspeter Kählig, Dennis Kurzbach, Rolf Breinbauer, Christian F. W. Becker
Publicado en: ChemBioChem, 2023, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300098

Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces

Autores: Ludovica Martina Epasto, Kateryna Che, Fanny Kozak, Albina Selimovic, Pavel Kaderavek, Dennis Kurzbach
Publicado en: Science Advances, 2022, ISSN 2375-2548
Editor: AAAS
DOI: 10.1126/sciadv.abq5179

Non-peer reviewed articles (1)

Hyperpolarized water as universal sensitivity booster in biomolecular NMR

Autores: Christian Hilty, Dennis Kurzbach, Lucio Frydman
Publicado en: Nature Protocols, 2022, ISSN 1754-2189
Editor: Nature Publishing Group
DOI: 10.1038/s41596-022-00693-8