Descripción del proyecto
El mapeo por asociación entre especies en los estudios de genómica
El método genético clásico no permite estudiar la variación entre especies, ya que no se dan cruces entre distintas especies y sus genomas suelen estar altamente reordenados. El objetivo del proyecto INTERACT, financiado con fondos europeos, es desarrollar métodos basados en la genómica para el mapeo por asociación (filogenética) a fin de descubrir señales en genomas altamente reordenados de distintas especies. El método se validará aplicándolo a las variaciones de los metabolitos secundarios de la familia de plantas «Brassicaceae». Los metabolitos secundarios presentan una gran variabilidad y tienen amplias aplicaciones en prevención del cáncer, control de plagas y diseño de alimentos. El trabajo y los resultados de este proyecto harán progresar significativamente la investigación genética.
Objetivo
During the past years, great progress has been made in connecting phenotypes to genotypes based on within-species variation. However, the more dramatic variation that can be found between species has not been explored for phenotype/genotype associations so-far. Using classical genetics to mine between-species variation is mostly impossible, because crosses between distinct species hardly work and their genomes are usually highly rearranged.
The goal of this project is to develop unprecedented genomics-based methods for inter-species (phylogenetic) association mapping, which can find signals even in highly re-arranged genomes of different species. To ensure that these methods are also useful in practice, we will apply them to the variation in secondary metabolites within the Brassicaceae plant family. Secondary metabolites are highly variable, genetically controlled, easy to measure and have broad application in cancer prevention, pest control and food design. Given the great potential of phylogenetic association mapping in general and secondary metabolites in particular, our work promises to be ground-breaking and have profound impact on many different fields of genetic research.
Specifically, our work plan includes the following points:
I) We will develop strategies for phylogenetic association mapping and implement them in publicly-available software.
II) We will establish a panel of inbred lines from ~200 Brassicaceae species and generate whole-genome assemblies for each of them.
III) We will exemplify the usefulness of phylogenetic association mapping by correlating the diversity of secondary metabolites to the differences in the respective genomes and validate the results by transforming or mutating candidate genes in appropriate species.
Ámbito científico
Palabras clave
Programa(s)
Régimen de financiación
ERC-STG - Starting GrantInstitución de acogida
80539 MUNCHEN
Alemania