High-throughput single-cell phenotypic analysis of functional antibody repertoires

Insights into the relationship between persistent antibody secretion and metabolic programming – A question for single-cell analysis

Author(s): Olivia T M, Bucheli; Klaus, Eyer
Published in: Immunology letters, 260, Issue 1, 2023, ISSN 0165-2478
Publisher: Elsevier BV
DOI: 10.1016/j.imlet.2023.06.006

Antibodies, repertoires and microdevices in antibody discovery and characterization

Author(s): Luca Johannes Schlotheuber, Ines Lüchtefeld, Klaus Eyer
Published in: Lab on a Chip, 2024, ISSN 1473-0197
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3lc00887h

The impact of frost-damage on the quality and quantity of the secreted antigen-specific IgG repertoire

Author(s): Magda Rybczynska, Jean Baudry, Eyer Klaus
Published in: Vaccine, Issue 38/33, 2020, Page(s) 5337-5342, ISSN 0264-410X
Publisher: Elsevier BV
DOI: 10.1016/j.vaccine.2020.05.066

High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics

Author(s): Annabelle Gérard, Adam Woolfe, Guillaume Mottet, Marcel Reichen, Carlos Castrillon, Vera Menrath, Sami Ellouze, Adeline Poitou, Raphaël Doineau, Luis Briseno-Roa, Pablo Canales-Herrerias, Pascaline Mary, Gregory Rose, Charina Ortega, Matthieu Delincé, Sosthene Essono, Bin Jia, Bruno Iannascoli, Odile Richard-Le Goff, Roshan Kumar, Samantha N. Stewart, Yannick Pousse, Bingqing Shen, Kevin Grosse
Published in: Nature Biotechnology, Issue 38/6, 2020, Page(s) 715-721, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-020-0466-7

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Author(s): Aline Linder, Kevin Portmann, Luca Johannes Schlotheuber, Alessandro Streuli, Wiona Sophie Glänzer, Klaus Eyer, Ines Lüchtefeld
Published in: JoVE, 2024, ISSN 1940-087X
Publisher: MYJoVE Corporation

Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centers

Author(s): Marco Molari, Klaus Eyer, Jean Baudry, Simona Cocco, Rémi Monasson
Published in: eLife, Issue 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.55678

The Quantitative Assessment of the Secreted IgG Repertoire after Recall to Evaluate the Quality of Immunizations

Author(s): Klaus Eyer, Carlos Castrillon, Guilhem Chenon, Jérôme Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry
Published in: The Journal of Immunology, Issue 205/4, 2020, Page(s) 1176-1184, ISSN 0022-1767
Publisher: American Association of Immunologists
DOI: 10.4049/jimmunol.2000112

High-affinity autoreactive plasma cells disseminate through multiple organs in patients with immune thrombocytopenic purpura

Author(s): Pablo Canales-Herrerias, Etienne Crickx, Matteo Broketa, Aurélien Sokal, Guilhem Chenon, Imane Azzaoui, Alexis Vandenberghe, Angga Perima, Bruno Iannascoli, Odile Richard-Le Goff, Carlos Castrillon, Guillaume Mottet, Delphine Sterlin, Ailsa Robbins, Marc Michel, Patrick England, Gael A. Millot, Klaus Eyer, Jean Baudry, Matthieu Mahevas, and Pierre Bruhns
Published in: The Journal of Clinical Investigation, 2022, ISSN 0021-9738
Publisher: American Society for Clinical Investigation
DOI: 10.1172/jci153580

Measuring single‐cell protein secretion in immunology –Technologies, advances and applications

Author(s): Bucheli T. M. Olivia, Sigvaldadóttir Ingibjörg, Eyer Klaus
Published in: European Journal of Immunology, 2021, ISSN 0014-2980
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202048976

Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay

Author(s): Millie Heo, Guilhem Chenon, Carlos Castrillon, Jérôme Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry, Klaus Eyer
Published in: Communications Biology, Issue 3/1, 2020, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-020-01296-3

Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap

Author(s): Bounab Y., Eyer K., Dixneuf S., Rybczynska M., Chauvel C., Mistretta M., Tran T., Aymerich N., Chenon G., Llitjos J.F., Venet F., Monneret G., Gillespie I., Cortez P., Moucadel V., Pachot A., Troesch A., Leissner P., Textoris J., Bibette J., Guyard C., Baudry J., Griffiths A.D. and Védrine C.
Published in: Nature Protocols, Issue 15, 2020, Page(s) 2920–2955, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-020-0354-0

Advancing single-cell technologies to directly correlate metabolic fluxes and functionalities in B cells during an immune response

Author(s): Bucheli, Olivia T.M.
Published in: 2023
Publisher: ETH Zürich
DOI: 10.3929/ethz-b-000642569

Functional humoral responses towards bacteria : complement activation at the single antibody level

Author(s): Nathan Aymerich
Published in: 2023
Publisher: ESPCI ParisTech

A novel Approach to Cytokine Release using Dynamic Multiplexed Single-Cell Analysis

Author(s): Portmann Kevin
Published in: 2023
Publisher: ETH Zürich
DOI: 10.3929/ethz-b-000634430

A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level

Author(s): Nathan Aymerich, Olivia T. M. Bucheli, Kevin Portmann, Klaus Eyer, and Jean Baudry
Published in: Microfluidics Diagnostics: Methods and Protocols., 2024
Publisher: Springer Nature

Biochemical Analysis of Secreted Molecules by Individual Cells

Author(s): O. T. M. Bucheli, I. Sigvaldadóttir, K. Eyer
Published in: Handbook of Single Cell Technologies, 2020, Page(s) 1-24, ISBN 978-981-10-4857-9
Publisher: Springer Nature Singapore Pte Ltd 2020
DOI: 10.1007/978-981-10-4857-9

One by One – Insights into Complex Immune Responses through Functional Single-cell Analysis

Author(s): Eyer, Klaus
Published in: CHIMIA International Journal for Chemistry,, Issue Volume 74, Number 9, September 2020, 2020, Page(s) 716-723(8), ISSN 0009-4293
Publisher: Schweizerische Chemische Gedellschaft
DOI: 10.2533/chimia.2020.716

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