High-throughput single-cell phenotypic analysis of functional antibody repertoires

Insights into the relationship between persistent antibody secretion and metabolic programming – A question for single-cell analysis

Autores: Olivia T M, Bucheli; Klaus, Eyer
Publicado en: Immunology letters, 260, Edición 1, 2023, ISSN 0165-2478
Editor: Elsevier BV
DOI: 10.1016/j.imlet.2023.06.006

Antibodies, repertoires and microdevices in antibody discovery and characterization

Autores: Luca Johannes Schlotheuber, Ines Lüchtefeld, Klaus Eyer
Publicado en: Lab on a Chip, 2024, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/d3lc00887h

The impact of frost-damage on the quality and quantity of the secreted antigen-specific IgG repertoire

Autores: Magda Rybczynska, Jean Baudry, Eyer Klaus
Publicado en: Vaccine, Edición 38/33, 2020, Página(s) 5337-5342, ISSN 0264-410X
Editor: Elsevier BV
DOI: 10.1016/j.vaccine.2020.05.066

High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics

Autores: Annabelle Gérard, Adam Woolfe, Guillaume Mottet, Marcel Reichen, Carlos Castrillon, Vera Menrath, Sami Ellouze, Adeline Poitou, Raphaël Doineau, Luis Briseno-Roa, Pablo Canales-Herrerias, Pascaline Mary, Gregory Rose, Charina Ortega, Matthieu Delincé, Sosthene Essono, Bin Jia, Bruno Iannascoli, Odile Richard-Le Goff, Roshan Kumar, Samantha N. Stewart, Yannick Pousse, Bingqing Shen, Kevin Grosse
Publicado en: Nature Biotechnology, Edición 38/6, 2020, Página(s) 715-721, ISSN 1087-0156
Editor: Nature Publishing Group
DOI: 10.1038/s41587-020-0466-7

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Autores: Aline Linder, Kevin Portmann, Luca Johannes Schlotheuber, Alessandro Streuli, Wiona Sophie Glänzer, Klaus Eyer, Ines Lüchtefeld
Publicado en: JoVE, 2024, ISSN 1940-087X
Editor: MYJoVE Corporation

Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centers

Autores: Marco Molari, Klaus Eyer, Jean Baudry, Simona Cocco, Rémi Monasson
Publicado en: eLife, Edición 9, 2020, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.55678

The Quantitative Assessment of the Secreted IgG Repertoire after Recall to Evaluate the Quality of Immunizations

Autores: Klaus Eyer, Carlos Castrillon, Guilhem Chenon, Jérôme Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry
Publicado en: The Journal of Immunology, Edición 205/4, 2020, Página(s) 1176-1184, ISSN 0022-1767
Editor: American Association of Immunologists
DOI: 10.4049/jimmunol.2000112

High-affinity autoreactive plasma cells disseminate through multiple organs in patients with immune thrombocytopenic purpura

Autores: Pablo Canales-Herrerias, Etienne Crickx, Matteo Broketa, Aurélien Sokal, Guilhem Chenon, Imane Azzaoui, Alexis Vandenberghe, Angga Perima, Bruno Iannascoli, Odile Richard-Le Goff, Carlos Castrillon, Guillaume Mottet, Delphine Sterlin, Ailsa Robbins, Marc Michel, Patrick England, Gael A. Millot, Klaus Eyer, Jean Baudry, Matthieu Mahevas, and Pierre Bruhns
Publicado en: The Journal of Clinical Investigation, 2022, ISSN 0021-9738
Editor: American Society for Clinical Investigation
DOI: 10.1172/jci153580

Measuring single‐cell protein secretion in immunology –Technologies, advances and applications

Autores: Bucheli T. M. Olivia, Sigvaldadóttir Ingibjörg, Eyer Klaus
Publicado en: European Journal of Immunology, 2021, ISSN 0014-2980
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202048976

Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay

Autores: Millie Heo, Guilhem Chenon, Carlos Castrillon, Jérôme Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry, Klaus Eyer
Publicado en: Communications Biology, Edición 3/1, 2020, ISSN 2399-3642
Editor: Springer Nature
DOI: 10.1038/s42003-020-01296-3

Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap

Autores: Bounab Y., Eyer K., Dixneuf S., Rybczynska M., Chauvel C., Mistretta M., Tran T., Aymerich N., Chenon G., Llitjos J.F., Venet F., Monneret G., Gillespie I., Cortez P., Moucadel V., Pachot A., Troesch A., Leissner P., Textoris J., Bibette J., Guyard C., Baudry J., Griffiths A.D. and Védrine C.
Publicado en: Nature Protocols, Edición 15, 2020, Página(s) 2920–2955, ISSN 1754-2189
Editor: Nature Publishing Group
DOI: 10.1038/s41596-020-0354-0

Advancing single-cell technologies to directly correlate metabolic fluxes and functionalities in B cells during an immune response

Autores: Bucheli, Olivia T.M.
Publicado en: 2023
Editor: ETH Zürich
DOI: 10.3929/ethz-b-000642569

Functional humoral responses towards bacteria : complement activation at the single antibody level

Autores: Nathan Aymerich
Publicado en: 2023
Editor: ESPCI ParisTech

A novel Approach to Cytokine Release using Dynamic Multiplexed Single-Cell Analysis

Autores: Portmann Kevin
Publicado en: 2023
Editor: ETH Zürich
DOI: 10.3929/ethz-b-000634430

A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level

Autores: Nathan Aymerich, Olivia T. M. Bucheli, Kevin Portmann, Klaus Eyer, and Jean Baudry
Publicado en: Microfluidics Diagnostics: Methods and Protocols., 2024
Editor: Springer Nature

Biochemical Analysis of Secreted Molecules by Individual Cells

Autores: O. T. M. Bucheli, I. Sigvaldadóttir, K. Eyer
Publicado en: Handbook of Single Cell Technologies, 2020, Página(s) 1-24, ISBN 978-981-10-4857-9
Editor: Springer Nature Singapore Pte Ltd 2020
DOI: 10.1007/978-981-10-4857-9

One by One – Insights into Complex Immune Responses through Functional Single-cell Analysis

Autores: Eyer, Klaus
Publicado en: CHIMIA International Journal for Chemistry,, Edición Volume 74, Number 9, September 2020, 2020, Página(s) 716-723(8), ISSN 0009-4293
Editor: Schweizerische Chemische Gedellschaft
DOI: 10.2533/chimia.2020.716

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