High-throughput single-cell phenotypic analysis of functional antibody repertoires

Insights into the relationship between persistent antibody secretion and metabolic programming – A question for single-cell analysis

Autori: Olivia T M, Bucheli; Klaus, Eyer
Pubblicato in: Immunology letters, 260, Numero 1, 2023, ISSN 0165-2478
Editore: Elsevier BV
DOI: 10.1016/j.imlet.2023.06.006

Antibodies, repertoires and microdevices in antibody discovery and characterization

Autori: Luca Johannes Schlotheuber, Ines Lüchtefeld, Klaus Eyer
Pubblicato in: Lab on a Chip, 2024, ISSN 1473-0197
Editore: Royal Society of Chemistry
DOI: 10.1039/d3lc00887h

The impact of frost-damage on the quality and quantity of the secreted antigen-specific IgG repertoire

Autori: Magda Rybczynska, Jean Baudry, Eyer Klaus
Pubblicato in: Vaccine, Numero 38/33, 2020, Pagina/e 5337-5342, ISSN 0264-410X
Editore: Elsevier BV
DOI: 10.1016/j.vaccine.2020.05.066

High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics

Autori: Annabelle Gérard, Adam Woolfe, Guillaume Mottet, Marcel Reichen, Carlos Castrillon, Vera Menrath, Sami Ellouze, Adeline Poitou, Raphaël Doineau, Luis Briseno-Roa, Pablo Canales-Herrerias, Pascaline Mary, Gregory Rose, Charina Ortega, Matthieu Delincé, Sosthene Essono, Bin Jia, Bruno Iannascoli, Odile Richard-Le Goff, Roshan Kumar, Samantha N. Stewart, Yannick Pousse, Bingqing Shen, Kevin Grosse
Pubblicato in: Nature Biotechnology, Numero 38/6, 2020, Pagina/e 715-721, ISSN 1087-0156
Editore: Nature Publishing Group
DOI: 10.1038/s41587-020-0466-7

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Autori: Aline Linder, Kevin Portmann, Luca Johannes Schlotheuber, Alessandro Streuli, Wiona Sophie Glänzer, Klaus Eyer, Ines Lüchtefeld
Pubblicato in: JoVE, 2024, ISSN 1940-087X
Editore: MYJoVE Corporation

Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centers

Autori: Marco Molari, Klaus Eyer, Jean Baudry, Simona Cocco, Rémi Monasson
Pubblicato in: eLife, Numero 9, 2020, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.55678

The Quantitative Assessment of the Secreted IgG Repertoire after Recall to Evaluate the Quality of Immunizations

Autori: Klaus Eyer, Carlos Castrillon, Guilhem Chenon, Jérôme Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry
Pubblicato in: The Journal of Immunology, Numero 205/4, 2020, Pagina/e 1176-1184, ISSN 0022-1767
Editore: American Association of Immunologists
DOI: 10.4049/jimmunol.2000112

High-affinity autoreactive plasma cells disseminate through multiple organs in patients with immune thrombocytopenic purpura

Autori: Pablo Canales-Herrerias, Etienne Crickx, Matteo Broketa, Aurélien Sokal, Guilhem Chenon, Imane Azzaoui, Alexis Vandenberghe, Angga Perima, Bruno Iannascoli, Odile Richard-Le Goff, Carlos Castrillon, Guillaume Mottet, Delphine Sterlin, Ailsa Robbins, Marc Michel, Patrick England, Gael A. Millot, Klaus Eyer, Jean Baudry, Matthieu Mahevas, and Pierre Bruhns
Pubblicato in: The Journal of Clinical Investigation, 2022, ISSN 0021-9738
Editore: American Society for Clinical Investigation
DOI: 10.1172/jci153580

Measuring single‐cell protein secretion in immunology –Technologies, advances and applications

Autori: Bucheli T. M. Olivia, Sigvaldadóttir Ingibjörg, Eyer Klaus
Pubblicato in: European Journal of Immunology, 2021, ISSN 0014-2980
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202048976

Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay

Autori: Millie Heo, Guilhem Chenon, Carlos Castrillon, Jérôme Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry, Klaus Eyer
Pubblicato in: Communications Biology, Numero 3/1, 2020, ISSN 2399-3642
Editore: Springer Nature
DOI: 10.1038/s42003-020-01296-3

Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap

Autori: Bounab Y., Eyer K., Dixneuf S., Rybczynska M., Chauvel C., Mistretta M., Tran T., Aymerich N., Chenon G., Llitjos J.F., Venet F., Monneret G., Gillespie I., Cortez P., Moucadel V., Pachot A., Troesch A., Leissner P., Textoris J., Bibette J., Guyard C., Baudry J., Griffiths A.D. and Védrine C.
Pubblicato in: Nature Protocols, Numero 15, 2020, Pagina/e 2920–2955, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/s41596-020-0354-0

Advancing single-cell technologies to directly correlate metabolic fluxes and functionalities in B cells during an immune response

Autori: Bucheli, Olivia T.M.
Pubblicato in: 2023
Editore: ETH Zürich
DOI: 10.3929/ethz-b-000642569

Functional humoral responses towards bacteria : complement activation at the single antibody level

Autori: Nathan Aymerich
Pubblicato in: 2023
Editore: ESPCI ParisTech

A novel Approach to Cytokine Release using Dynamic Multiplexed Single-Cell Analysis

Autori: Portmann Kevin
Pubblicato in: 2023
Editore: ETH Zürich
DOI: 10.3929/ethz-b-000634430

A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level

Autori: Nathan Aymerich, Olivia T. M. Bucheli, Kevin Portmann, Klaus Eyer, and Jean Baudry
Pubblicato in: Microfluidics Diagnostics: Methods and Protocols., 2024
Editore: Springer Nature

Biochemical Analysis of Secreted Molecules by Individual Cells

Autori: O. T. M. Bucheli, I. Sigvaldadóttir, K. Eyer
Pubblicato in: Handbook of Single Cell Technologies, 2020, Pagina/e 1-24, ISBN 978-981-10-4857-9
Editore: Springer Nature Singapore Pte Ltd 2020
DOI: 10.1007/978-981-10-4857-9

One by One – Insights into Complex Immune Responses through Functional Single-cell Analysis

Autori: Eyer, Klaus
Pubblicato in: CHIMIA International Journal for Chemistry,, Numero Volume 74, Number 9, September 2020, 2020, Pagina/e 716-723(8), ISSN 0009-4293
Editore: Schweizerische Chemische Gedellschaft
DOI: 10.2533/chimia.2020.716

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