Descripción del proyecto
Estudio sobre cómo las bacterias orales repercuten en la salud respiratoria
Dado que la cavidad oral es la entrada a las vías respiratorias inferiores, es posible que las bacterias orales desempeñen algún papel en la salud pulmonar. Parece que las bacterias con lipopolisacáridos (LPS) proinflamatorios potentes se encuentran con mayor frecuencia en los pulmones de las personas asmáticas que en los de las sanas. El proyecto financiado con fondos europeos BRuSH probará la hipótesis de que las comunidades de microbioma dominadas por bacterias que producen LPS que inducen una respuesta inmunitaria proinflamatoria especialmente fuerte en el huésped repercuten negativamente en la salud respiratoria. Determinará si una composición bacteriana específica y los tipos de bacterias que producen LPS en muestras orales y de polvo predicen la función pulmonar y la salud respiratoria a lo largo del tiempo, y si los distintos tipos de bacterias que producen LPS afectan a los LPS de la saliva y el polvo. El proyecto abrirá el camino a una mejor salud oral.
Objetivo
The oral cavity is the gateway to the lower respiratory tract, and oral bacteria are likely to play a role in lung health. This may be the case for pathogens as well as commensal bacteria and the balance between species. The oral bacterial community of patients with periodontitis is dominated by gram-negative bacteria and a higher lipopolysaccharide (LPS) activity than in healthy microbiota. Furthermore, bacteria with especially potent pro-inflammatory LPS have been shown to be more common in the lungs of asthmatic than in healthy individuals. The working hypothesis of BRuSH is that microbiome communities dominated by LPS-producing bacteria which induce a particularly strong pro-inflammatory immune response in the host, will have a negative effect on respiratory health. I will test this hypothesis in two longitudinally designed population-based lung health studies. I aim to identify whether specific bacterial composition and types of LPS producing bacteria in oral and dust samples predict lung function and respiratory health over time; and if the different types of LPS-producing bacteria affect LPS in saliva saliva and dust. BRuSH will apply functional genome annotation that can assign biological significance to raw bacterial DNA sequences. With this bioinformatics tool I will cluster microbiome data into various LPS-producers: bacteria with LPS with strong inflammatory effects and others with weak- or antagonistic effects. The epidemiological studies will be supported by mice-models of asthma and cell assays of human bronchial epithelial cells, by exposing mice and bronchial cells to chemically synthesized Lipid A (the component that drive the LPS-induced immune responses) of various potency. The goal of BRuSH is to prove a causal relationship between oral microbiome and lung health, and gain knowledge that will enable us to make oral health a feasible target for intervention programs aimed at optimizing lung health and preventing respiratory disease.
Ámbito científico
- medical and health sciencesclinical medicinepneumologyasthma
- natural sciencesbiological sciencesmicrobiologybacteriology
- medical and health sciencesbasic medicineimmunology
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- medical and health scienceshealth sciencesinfectious diseasesRNA virusescoronaviruses
Palabras clave
Programa(s)
Régimen de financiación
ERC-STG - Starting GrantInstitución de acogida
5020 Bergen
Noruega