Description du projet
Une méthode innovante de détection des endotoxines par nanotechnologie
Les règlements de sécurité exigent une détection quantitative objective des endotoxines bactériennes dans les produits destinés à un usage médical et au développement de médicaments. La fiabilité des tests actuels au lysat d’amébocytes de limule (LAL) est limitée et ils ne peuvent pas être appliqués à tous les produits et substances. Financé par le programme Actions Marie Skłodowska-Curie, le projet ENDONANO exploitera un nouveau concept basé sur la capacité des nanoparticules métalliques à adsorber les endotoxines ainsi que des méthodes de détection basées sur des balises moléculaires. Il a pour but de développer un test de quantification des endotoxines qui fonctionne dans des matrices complexes et dans une vaste gamme de conditions. ENDONANO formera quatre chercheurs doctorants, en leur proposant des cours portant sur des compétences scientifiques, techniques et transférables, une participation à des événements scientifiques et une mise en réseau.
Objectif
ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e. the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,
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MSCA-ITN - Marie Skłodowska-Curie Innovative Training Networks (ITN)Coordinateur
00185 Roma
Italie