Descrizione del progetto
Un rilevamento innovativo di endotossine mediante nanotecnologia
Le norme di sicurezza richiedono la rilevazione quantitativa imparziale delle endotossine batteriche nei prodotti destinati all’uso medico e allo sviluppo di farmaci. Gli attuali esami sul lisato di amebociti di Limulus (LAL) presentano alcuni limiti di affidabilità e non possono essere applicati a tutti i prodotti e le sostanze. Il progetto ENDONANO, finanziato dal programma di azioni Marie Skłodowska-Curie, si avvarrà di un nuovo concetto basato sulla capacità delle nanoparticelle metalliche di adsorbire endotossine, nonché di metodi di rilevamento basati su fari molecolari. L’obiettivo è sviluppare un esame di quantificazione delle endotossine che funzioni in matrici complesse e in un’ampia gamma di condizioni. ENDONANO formerà quattro dottorandi, fornendo corsi in competenze scientifiche, tecniche e trasferibili, la partecipazione a eventi scientifici e opportunità di collegamenti in rete.
Obiettivo
ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e. the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,
Campo scientifico
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-ITN - Marie Skłodowska-Curie Innovative Training Networks (ITN)Coordinatore
00185 Roma
Italia