Continuous Downstream Processing of Bioproducts

Project Information

CODOBIO

Grant agreement ID: 812909

DOI

10.3030/812909

Project closed

EC signature date 30 August 2018

Start date 1 February 2019

End date 31 August 2023

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 3 988 839,25

EU contribution

€ 3 988 839,25

Coordinated by

ACIB GMBH
Austria

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (16)

Batch and continuous chromatographic purification at microfluidic, lab and pilot scale

Report on the comparison between batch and continuous chromatographic purification of the model bioproducts at microfluidic lab and pilot scale

Mixing in microscale

Report on the experimental results of mixing in microscale for the prediction of lab and pilot scale processes

Small scale analysis

Report on analysis on very small scale

On-line estimation of product quality

Report on experimental results of online estimation of product quality

Power input and energy dissipation in miniaturized systems

Report on the determination of power input and energy dissipation in miniaturized systems.

Simulation of chromatographic behaviour for continuous DSP

Report on the simulation of chromatographic behaviour for continuous downstream processing

PAT for integrated processes

Report on use of PAT tools for the rapid development of the integrated processes

Integrated continuous processes: economic impact, drivers and future

Report on the economic impact and drivers of integrated continuous processes and how such technology can support future challenges in biopharmaceutical industry

HTS of integrated processes

Report on the use on miniaturization for the high throughput screening of integrated processes

Expanded bed technology in continuous processes

Report on the use of expanded bed technology in continuous processes including pilot scale processing

Purification of antibody in batch and continuous

Report on the experimental results of purification of antibody in batch and continuous mode

Integration of continuous downstream processes II

Report on the integration of continuous downstream processes process feasibility studies and economic validation

Integration of continuous downstream processes I

Report on the integration of continuous downstream processes development of integrated control strategies and automation

Microfluidic liquid-liquid extraction

Report on experimental results of liquidliquid extraction of industrial enzymes in a microfluidic platform

Batch and continuous extraction at microfluidic, lab and pilot scale

Report on the comparison between batch and continuous extraction of industrial enzymes at microfluidic lab and pilot scale including economic and environmental analysis

Effect of RTD on product collection

Report on the effect of residence time distribution on startup and shutdown of product collection together with mathematical algorithm for decision making aid

Other (4)

Supervisory Board

Supervisory Board of the network constituted.

Recruitment procedure I

Vacancies for ESR recruitment advertised.

Final and press conference

Final and press conference.

Miniaturized HTS

Training course on miniaturized HTS.

Websites, patent fillings, videos etc. (2)

Dissemination contents/materials

Dissemination contentsmaterials produced published and available to stakeholders ie scientific community and general public via website

Website

Dedicated project website launched.

Publications

Peer reviewed articles (30)

White paper on high‐throughput process development for integrated continuous biomanufacturing

Author(s): Mariana N. São Pedro, Tiago C. Silva, Rohan Patil, Marcel Ottens
Published in: Biotechnology and Bioengineering, 2021, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27757

Advanced control strategies for bioprocess chromatography: Challenges and opportunities for intensified processes and next generation products

Author(s): Alexander Armstrong, Kieran Horry, Tingting Cui, Martyn Hulley, Richard Turner, Suzanne S. Farid, Stephen Goldrick, Daniel G. Bracewell
Published in: Journal of Chromatography A, Issue 1639, 2021, Page(s) 461914, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2021.461914

An integrated microfluidic device for continuous bioprocessing

Author(s): Malik Abdul Wahab, C. Domingues, Ana M. Azevedo, Virginia Chu, Joao Pedro Conde, M. Raquel Aires-Barros
Published in: Separation and Purification Technology, Issue 332, 2024, ISSN 1383-5866
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2023.125702

Process analytical technique (PAT) miniaturization for monoclonal antibody aggregate detection incontinuous downstream processing

Author(s): São Pedro, M.N., Klijn, M.E., Eppink, M.H. and Ottens, M
Published in: Journal of Chemical Technology and Biotechnology, Issue 97, 2022, Page(s) 2347-2364, ISSN 0268-2575
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6920

Design of millidevices to expedite apparent solubility measurements

Author(s): Maria del Carme Pons Royo; Jean-Luc Beulay; Eric Valery; Alois Jungbauer; Peter Satzer
Published in: Reaction Chemistry and Engineering, Issue 7 (9), 2022, Page(s) 2045 - 2053, ISSN 2058-9883
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2re00022a

Methodology for fast development of digital solutions in integrated continuous downstream processing

Author(s): Niklas Andersson, Joaquín Gomis Fons, Madelène Isaksson, Simon Tallvod, Daniel Espinoza, Linnea Sjökvist, Gusten Zandler Andersson, Bernt Nilsson
Published in: Biotechnology and Bioengineering, 2023, Page(s) 10, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28501

Microchromatography integrated with impedance sensor for bioprocess optimization: Experimental and numerical study of column efficiency for evaluation of scalability

Author(s): Amin Javidanbardan, Virginia Chu, João P. Conde, Ana M. Azevedo,
Published in: Journal of Chromatography A, Issue 1661, 2022, Page(s) 462678, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2021.462678

A fluidized-bed-riser adsorption system for continuous bioproduct recovery from crude feedstock

Author(s): Herlevi L.-M., Fernandez-Lahore M., Ferreira G.
Published in: Biotechnology and Bioengineering, Issue 120, 10, 2023, Page(s) 2969 - 2976, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28458

Online optimization of dynamic binding capacity and productivity by model predictive control

Author(s): Touraj Eslami, Martin Steinberger, Christian Csizmazia, Alois Jungbauer, Nico Lingg
Published in: Journal of Chromatography A, Issue Volume 1680, 13 September 2022, 463420, 2022, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2022.463420

Small, smaller, smallest: Miniaturization of chromatographic process development

Author(s): Silva, TC, Eppink, M, Ottens, M
Published in: Journal of Chromatography A, Issue 1681, 2022, Page(s) 463451, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2022.463451

Mode and dosage time in polyethylene glycol precipitation process influences protein precipitate size and filterability

Author(s): Maria del Carme Pons Royo; Jean-Luc Beulay: Eric Valery; Alois Jungbauer; Peter Satzer
Published in: Process Biochemistry, Issue 114, 2022, Page(s) 77-85, ISSN 1359-5113
Publisher: Elsevier Applied Science
DOI: 10.1016/j.procbio.2022.01.017

Soft Sensor based on Raman Spectroscopy for the In-Line Monitoring of Metabolites and Polymer Quality in the Biomanufacturing of Polyhydroxyalkanoates

Author(s): João Medeiros Garcia Alcântara; Francesco Iannacci; Massimo Morbidelli; Mattia Sponchioni
Published in: Journal of Biotechnology, 2023, ISSN 0168-1656
Publisher: Elsevier BV
DOI: 10.1016/j.jbiotec.2023.10.005

Automation and miniaturization: enabling tools for fast, high‐throughput process development in integrated continuous biomanufacturing

Author(s): Tiago Castanheira Silva, Michel Eppink, Marcel Ottens
Published in: Journal of Chemical Technology & Biotechnology, 2021, ISSN 0268-2575
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6792

Moving adsorption belt system for continuous bioproduct recovery utilizing composite fibrous adsorbents

Author(s): Guo Y., Kangwa M., Ali W., Mayer-Gall T., Gutmann J.S., Zenneck C., Winter M., Jungbauer A., Fernandez Lahore H.M.
Published in: Frontiers in Bioengineering and Biotechnology, Issue 11, art. no. 1135447, 2023, ISSN 2296-4185
Publisher: Frontiers Media S.A
DOI: 10.3389/fbioe.2023.1135447

Design of a microfluidic mixer channel: First steps into creating a fluorescent dye-based biosensor for mAb aggregate detection

Author(s): São Pedro, M. N., Santos, M.S., Eppink M.H.M., & Ottens, M
Published in: Biotechnology Journal, Issue 18(1), 2023, Page(s) 2200332, ISSN 1860-7314
Publisher: Wiley-VCH GmbH
DOI: 10.1002/biot.202200332

Continuous precipitation of antibodies by feeding of solid polyethylene glycol

Author(s): Maria del Carme Pons Royo; Tommaso De Santis: Daniel Komuczki; Peter Satzer: Alois Jungbauer
Published in: Separation and Purification Technology, Issue 304, 2023, ISSN 1383-5866
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2022.122373

Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates

Author(s): João Medeiros Garcia Alcântara, Francesco Distante, Giuseppe Storti, Davide Moscatelli, Massimo Morbidelli, Mattia Sponchioni
Published in: Biotechnology Advances, Issue 42, 2020, Page(s) 107582, ISSN 0734-9750
Publisher: Elsevier BV
DOI: 10.1016/j.biotechadv.2020.107582

Characterization of hydrodynamics and volumetric power input in microtiter plates for the scale-up of downstream operations

Author(s): Ignacio Montes-Serrano; Peter Satzer; Alois Jungbauer; Astrid Dürauer
Published in: Biotechnology and Bioengineering, Issue 119 (2), 2022, Page(s) 523 - 534, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27983

Residence time distribution in counter-current protein A affinity chromatography using an inert tracer

Author(s): Narges Lali; Peter Satzer; Alois Jungbauer
Published in: Journal of Chromatography A, Issue 1683, 2022, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2022.463530

Traceability of products and guide for batch definition in integrated continuous biomanufacturing

Author(s): Narges Lali; Alois Jungbauer; Peter Satzer
Published in: Journal of Chemical Technology and Biotechnology, Issue 97 (9), 2022, Page(s) 2386 - 2392, ISSN 0268-2575
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6953

An automated buffer management system for small-scale continuous downstream bioprocessing

Author(s): Madelène Isaksson, Joaquín Gomis-Fons, Niklas Andersson, Bernt Nilsson
Published in: Journal of Chromatography A, Issue 1695, 2023, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2023.463942

Milliscale reactors for integration of continuous precipitation and filtration

Author(s): Maria del Carme Pons Royo; Ignacio Montes‐Serrano; Eric Valery; Alois Jungbauer; Peter Satzer
Published in: Journal of Chemical Technology and Biotechnology, Issue 97 (11), 2022, Page(s) 3183 - 3192, ISSN 1097-4660
Publisher: John Wiley and Sons Ltd
DOI: 10.1002/jctb.7187

A modelling approach for volumetric power input in the microscale and its utilization for the scale-up of solid-liquid mixing systems

Author(s): Ignacio Montes-Serrano: Daniel Paul Komuczki; Astrid Dürauer
Published in: Chemical Engineering and Processing: Process Intensification, Issue 184, 2023, ISSN 0255-2701
Publisher: Elsevier BV
DOI: 10.1016/j.cep.2023.109303

Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregates

Author(s): São Pedro, M.N., Eppink M.H.M., & Ottens, M
Published in: Biotechnology Progress, Issue e3355, 2023, ISSN 8756-7938
Publisher: American Chemical Society
DOI: 10.1002/btpr.3355

Productivity for free: Residence time gradients during loading increase dynamic binding capacity and productivity

Author(s): Touraj Eslami, Leo A. Jakob, Peter Satzer, Gerald Ebner, Alois Jungbauer, Nico Lingg
Published in: Separation and Purification Technology, Issue Volume 281, 15 January 2022, 119985, 2022, ISSN 1383-5866
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2021.119985

A Systematic Approach for Developing 3D High-Quality PDMS Microfluidic Chips Based on Micromilling Technology

Author(s): Amin Javidanbardan, Ana M. Azevedo, Virginia Chu, João P. Conde
Published in: Micromachines, Issue 13, 2022, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi13010006

Design of an integrated continuous downstream process for acid-sensitive monoclonal antibodies based on a calcium-dependent Protein A ligand

Author(s): Julia Scheffel, Madelène Isaksson, Joaquín Gomis-Fons , Hubert Schwarz, Niklas Andersson, Björn Norén, Anita Solbrand, Veronique Chotteau, Sophia Hober, Bernt Nilsson
Published in: Journal of Chromatography A, Issue 1664, 2022, Page(s) 1-18, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2022.462806

Megaporous monolithic adsorbents for bioproduct recovery as prepared on the basis of nonwoven fabrics

Author(s): Guo Y., Ali W., Schneider A., Salma A., Mayer-Gall T., Gutmann J.S., Fernandez Lahore H.M.
Published in: Electrophoresis, Issue 43, issue 13-14, 2022, Page(s) 1387 - 1398, ISSN 0173-0835
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/elps.202100220

Real-time detection of mAb aggregates in an integrated downstream process

Author(s): São Pedro, M.N., Isaksson, M., Gomis-Fons, J., Eppink M.H.M., Nilsson, B. & Ottens, M.
Published in: Biotechnology & Bioengineering, Issue 120 (10), 2023, Page(s) 2989-3000, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28466

Integrated continuous biomanufacturing on pilot scale for acid‐sensitive monoclonal antibodies

Author(s): Hubert Schwarz; Joaquín Gomis‐Fons; Madelène Isaksson; Julia Scheffel; Niklas Andersson; Andreas Andersson; Andreas Castan; Anita Solbrand; Sophia Hober; Bernt Nilsson; Veronique Chotteau
Published in: Biotechnology and Bioengineering, Issue 3, 2022, Page(s) 1-18, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28120

Intellectual Property Rights

Other (2)

Apparatus and method for the purification of biomolecules

Application/Publication number: WO 2022083901
Date: 2021-06-15
Applicant(s): NOVASEP PROCESS

APPARATUS AND METHOD FOR REMOVING PROTEINS TAKEN UP IN A CARRIER LIQUID

Application/Publication number: WO 2022207907A1
Date: 2021-04-01
Applicant(s): CONSTRUCTOR UNIVERSITY BREMEN GGMBH