Continuous Downstream Processing of Bioproducts

Informations projet

CODOBIO

N° de convention de subvention: 812909

DOI

10.3030/812909

Projet clôturé

Date de signature de la CE 30 Août 2018

Date de début 1 Février 2019

Date de fin 31 Août 2023

Financé au titre de

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coût total

€ 3 988 839,25

Contribution de l’UE

€ 3 988 839,25

Coordonné par

ACIB GMBH
Austria

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (16)

Batch and continuous chromatographic purification at microfluidic, lab and pilot scale

Report on the comparison between batch and continuous chromatographic purification of the model bioproducts at microfluidic lab and pilot scale

Mixing in microscale

Report on the experimental results of mixing in microscale for the prediction of lab and pilot scale processes

Small scale analysis

Report on analysis on very small scale

On-line estimation of product quality

Report on experimental results of online estimation of product quality

Power input and energy dissipation in miniaturized systems

Report on the determination of power input and energy dissipation in miniaturized systems.

Simulation of chromatographic behaviour for continuous DSP

Report on the simulation of chromatographic behaviour for continuous downstream processing

PAT for integrated processes

Report on use of PAT tools for the rapid development of the integrated processes

Integrated continuous processes: economic impact, drivers and future

Report on the economic impact and drivers of integrated continuous processes and how such technology can support future challenges in biopharmaceutical industry

HTS of integrated processes

Report on the use on miniaturization for the high throughput screening of integrated processes

Expanded bed technology in continuous processes

Report on the use of expanded bed technology in continuous processes including pilot scale processing

Purification of antibody in batch and continuous

Report on the experimental results of purification of antibody in batch and continuous mode

Integration of continuous downstream processes II

Report on the integration of continuous downstream processes process feasibility studies and economic validation

Integration of continuous downstream processes I

Report on the integration of continuous downstream processes development of integrated control strategies and automation

Microfluidic liquid-liquid extraction

Report on experimental results of liquidliquid extraction of industrial enzymes in a microfluidic platform

Batch and continuous extraction at microfluidic, lab and pilot scale

Report on the comparison between batch and continuous extraction of industrial enzymes at microfluidic lab and pilot scale including economic and environmental analysis

Effect of RTD on product collection

Report on the effect of residence time distribution on startup and shutdown of product collection together with mathematical algorithm for decision making aid

Other (4)

Supervisory Board

Supervisory Board of the network constituted.

Recruitment procedure I

Vacancies for ESR recruitment advertised.

Final and press conference

Final and press conference.

Miniaturized HTS

Training course on miniaturized HTS.

Websites, patent fillings, videos etc. (2)

Dissemination contents/materials

Dissemination contentsmaterials produced published and available to stakeholders ie scientific community and general public via website

Website

Dedicated project website launched.

Publications

Peer reviewed articles (30)

White paper on high‐throughput process development for integrated continuous biomanufacturing

Auteurs: Mariana N. São Pedro, Tiago C. Silva, Rohan Patil, Marcel Ottens
Publié dans: Biotechnology and Bioengineering, 2021, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27757

Advanced control strategies for bioprocess chromatography: Challenges and opportunities for intensified processes and next generation products

Auteurs: Alexander Armstrong, Kieran Horry, Tingting Cui, Martyn Hulley, Richard Turner, Suzanne S. Farid, Stephen Goldrick, Daniel G. Bracewell
Publié dans: Journal of Chromatography A, Numéro 1639, 2021, Page(s) 461914, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2021.461914

An integrated microfluidic device for continuous bioprocessing

Auteurs: Malik Abdul Wahab, C. Domingues, Ana M. Azevedo, Virginia Chu, Joao Pedro Conde, M. Raquel Aires-Barros
Publié dans: Separation and Purification Technology, Numéro 332, 2024, ISSN 1383-5866
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2023.125702

Process analytical technique (PAT) miniaturization for monoclonal antibody aggregate detection incontinuous downstream processing

Auteurs: São Pedro, M.N., Klijn, M.E., Eppink, M.H. and Ottens, M
Publié dans: Journal of Chemical Technology and Biotechnology, Numéro 97, 2022, Page(s) 2347-2364, ISSN 0268-2575
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6920

Design of millidevices to expedite apparent solubility measurements

Auteurs: Maria del Carme Pons Royo; Jean-Luc Beulay; Eric Valery; Alois Jungbauer; Peter Satzer
Publié dans: Reaction Chemistry and Engineering, Numéro 7 (9), 2022, Page(s) 2045 - 2053, ISSN 2058-9883
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d2re00022a

Methodology for fast development of digital solutions in integrated continuous downstream processing

Auteurs: Niklas Andersson, Joaquín Gomis Fons, Madelène Isaksson, Simon Tallvod, Daniel Espinoza, Linnea Sjökvist, Gusten Zandler Andersson, Bernt Nilsson
Publié dans: Biotechnology and Bioengineering, 2023, Page(s) 10, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28501

Microchromatography integrated with impedance sensor for bioprocess optimization: Experimental and numerical study of column efficiency for evaluation of scalability

Auteurs: Amin Javidanbardan, Virginia Chu, João P. Conde, Ana M. Azevedo,
Publié dans: Journal of Chromatography A, Numéro 1661, 2022, Page(s) 462678, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2021.462678

A fluidized-bed-riser adsorption system for continuous bioproduct recovery from crude feedstock

Auteurs: Herlevi L.-M., Fernandez-Lahore M., Ferreira G.
Publié dans: Biotechnology and Bioengineering, Numéro 120, 10, 2023, Page(s) 2969 - 2976, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28458

Online optimization of dynamic binding capacity and productivity by model predictive control

Auteurs: Touraj Eslami, Martin Steinberger, Christian Csizmazia, Alois Jungbauer, Nico Lingg
Publié dans: Journal of Chromatography A, Numéro Volume 1680, 13 September 2022, 463420, 2022, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2022.463420

Small, smaller, smallest: Miniaturization of chromatographic process development

Auteurs: Silva, TC, Eppink, M, Ottens, M
Publié dans: Journal of Chromatography A, Numéro 1681, 2022, Page(s) 463451, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2022.463451

Mode and dosage time in polyethylene glycol precipitation process influences protein precipitate size and filterability

Auteurs: Maria del Carme Pons Royo; Jean-Luc Beulay: Eric Valery; Alois Jungbauer; Peter Satzer
Publié dans: Process Biochemistry, Numéro 114, 2022, Page(s) 77-85, ISSN 1359-5113
Éditeur: Elsevier Applied Science
DOI: 10.1016/j.procbio.2022.01.017

Soft Sensor based on Raman Spectroscopy for the In-Line Monitoring of Metabolites and Polymer Quality in the Biomanufacturing of Polyhydroxyalkanoates

Auteurs: João Medeiros Garcia Alcântara; Francesco Iannacci; Massimo Morbidelli; Mattia Sponchioni
Publié dans: Journal of Biotechnology, 2023, ISSN 0168-1656
Éditeur: Elsevier BV
DOI: 10.1016/j.jbiotec.2023.10.005

Automation and miniaturization: enabling tools for fast, high‐throughput process development in integrated continuous biomanufacturing

Auteurs: Tiago Castanheira Silva, Michel Eppink, Marcel Ottens
Publié dans: Journal of Chemical Technology & Biotechnology, 2021, ISSN 0268-2575
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6792

Moving adsorption belt system for continuous bioproduct recovery utilizing composite fibrous adsorbents

Auteurs: Guo Y., Kangwa M., Ali W., Mayer-Gall T., Gutmann J.S., Zenneck C., Winter M., Jungbauer A., Fernandez Lahore H.M.
Publié dans: Frontiers in Bioengineering and Biotechnology, Numéro 11, art. no. 1135447, 2023, ISSN 2296-4185
Éditeur: Frontiers Media S.A
DOI: 10.3389/fbioe.2023.1135447

Design of a microfluidic mixer channel: First steps into creating a fluorescent dye-based biosensor for mAb aggregate detection

Auteurs: São Pedro, M. N., Santos, M.S., Eppink M.H.M., & Ottens, M
Publié dans: Biotechnology Journal, Numéro 18(1), 2023, Page(s) 2200332, ISSN 1860-7314
Éditeur: Wiley-VCH GmbH
DOI: 10.1002/biot.202200332

Continuous precipitation of antibodies by feeding of solid polyethylene glycol

Auteurs: Maria del Carme Pons Royo; Tommaso De Santis: Daniel Komuczki; Peter Satzer: Alois Jungbauer
Publié dans: Separation and Purification Technology, Numéro 304, 2023, ISSN 1383-5866
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2022.122373

Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates

Auteurs: João Medeiros Garcia Alcântara, Francesco Distante, Giuseppe Storti, Davide Moscatelli, Massimo Morbidelli, Mattia Sponchioni
Publié dans: Biotechnology Advances, Numéro 42, 2020, Page(s) 107582, ISSN 0734-9750
Éditeur: Elsevier BV
DOI: 10.1016/j.biotechadv.2020.107582

Characterization of hydrodynamics and volumetric power input in microtiter plates for the scale-up of downstream operations

Auteurs: Ignacio Montes-Serrano; Peter Satzer; Alois Jungbauer; Astrid Dürauer
Publié dans: Biotechnology and Bioengineering, Numéro 119 (2), 2022, Page(s) 523 - 534, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27983

Residence time distribution in counter-current protein A affinity chromatography using an inert tracer

Auteurs: Narges Lali; Peter Satzer; Alois Jungbauer
Publié dans: Journal of Chromatography A, Numéro 1683, 2022, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2022.463530

Traceability of products and guide for batch definition in integrated continuous biomanufacturing

Auteurs: Narges Lali; Alois Jungbauer; Peter Satzer
Publié dans: Journal of Chemical Technology and Biotechnology, Numéro 97 (9), 2022, Page(s) 2386 - 2392, ISSN 0268-2575
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jctb.6953

An automated buffer management system for small-scale continuous downstream bioprocessing

Auteurs: Madelène Isaksson, Joaquín Gomis-Fons, Niklas Andersson, Bernt Nilsson
Publié dans: Journal of Chromatography A, Numéro 1695, 2023, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2023.463942

Milliscale reactors for integration of continuous precipitation and filtration

Auteurs: Maria del Carme Pons Royo; Ignacio Montes‐Serrano; Eric Valery; Alois Jungbauer; Peter Satzer
Publié dans: Journal of Chemical Technology and Biotechnology, Numéro 97 (11), 2022, Page(s) 3183 - 3192, ISSN 1097-4660
Éditeur: John Wiley and Sons Ltd
DOI: 10.1002/jctb.7187

A modelling approach for volumetric power input in the microscale and its utilization for the scale-up of solid-liquid mixing systems

Auteurs: Ignacio Montes-Serrano: Daniel Paul Komuczki; Astrid Dürauer
Publié dans: Chemical Engineering and Processing: Process Intensification, Numéro 184, 2023, ISSN 0255-2701
Éditeur: Elsevier BV
DOI: 10.1016/j.cep.2023.109303

Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregates

Auteurs: São Pedro, M.N., Eppink M.H.M., & Ottens, M
Publié dans: Biotechnology Progress, Numéro e3355, 2023, ISSN 8756-7938
Éditeur: American Chemical Society
DOI: 10.1002/btpr.3355

Productivity for free: Residence time gradients during loading increase dynamic binding capacity and productivity

Auteurs: Touraj Eslami, Leo A. Jakob, Peter Satzer, Gerald Ebner, Alois Jungbauer, Nico Lingg
Publié dans: Separation and Purification Technology, Numéro Volume 281, 15 January 2022, 119985, 2022, ISSN 1383-5866
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2021.119985

A Systematic Approach for Developing 3D High-Quality PDMS Microfluidic Chips Based on Micromilling Technology

Auteurs: Amin Javidanbardan, Ana M. Azevedo, Virginia Chu, João P. Conde
Publié dans: Micromachines, Numéro 13, 2022, ISSN 2072-666X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi13010006

Design of an integrated continuous downstream process for acid-sensitive monoclonal antibodies based on a calcium-dependent Protein A ligand

Auteurs: Julia Scheffel, Madelène Isaksson, Joaquín Gomis-Fons , Hubert Schwarz, Niklas Andersson, Björn Norén, Anita Solbrand, Veronique Chotteau, Sophia Hober, Bernt Nilsson
Publié dans: Journal of Chromatography A, Numéro 1664, 2022, Page(s) 1-18, ISSN 0021-9673
Éditeur: Elsevier BV
DOI: 10.1016/j.chroma.2022.462806

Megaporous monolithic adsorbents for bioproduct recovery as prepared on the basis of nonwoven fabrics

Auteurs: Guo Y., Ali W., Schneider A., Salma A., Mayer-Gall T., Gutmann J.S., Fernandez Lahore H.M.
Publié dans: Electrophoresis, Numéro 43, issue 13-14, 2022, Page(s) 1387 - 1398, ISSN 0173-0835
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/elps.202100220

Real-time detection of mAb aggregates in an integrated downstream process

Auteurs: São Pedro, M.N., Isaksson, M., Gomis-Fons, J., Eppink M.H.M., Nilsson, B. & Ottens, M.
Publié dans: Biotechnology & Bioengineering, Numéro 120 (10), 2023, Page(s) 2989-3000, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28466

Integrated continuous biomanufacturing on pilot scale for acid‐sensitive monoclonal antibodies

Auteurs: Hubert Schwarz; Joaquín Gomis‐Fons; Madelène Isaksson; Julia Scheffel; Niklas Andersson; Andreas Andersson; Andreas Castan; Anita Solbrand; Sophia Hober; Bernt Nilsson; Veronique Chotteau
Publié dans: Biotechnology and Bioengineering, Numéro 3, 2022, Page(s) 1-18, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28120

Droits de propriété intellectuelle

Other (2)

Apparatus and method for the purification of biomolecules

Numéro de demande/publication: WO 2022083901
Date: 2021-06-15
Demandeur(s): NOVASEP PROCESS

APPARATUS AND METHOD FOR REMOVING PROTEINS TAKEN UP IN A CARRIER LIQUID

Numéro de demande/publication: WO 2022207907A1
Date: 2021-04-01
Demandeur(s): CONSTRUCTOR UNIVERSITY BREMEN GGMBH