Project description
A unique approach to improving biomarker fingerprinting
Tracking biomarker origin is increasingly important in many different fields, such as life, environmental, food and forensic sciences. Combining metabolomics and fluxomics with isotopic fingerprinting shows great potential towards achieving this aim. Isotopic analysis by nuclear magnetic resonance (NMR) allows scientists to quantify with great precision the differences of isotopic contents on each site of a molecule. The EU-funded SUMMIT project has been established to address the current limitations of 13C isotopic NMR analysis. By using two powerful NMR methods – dissolution dynamic nuclear polarisation and ultrafast 2D NMR – the project will allow scientists to simultaneously measure 13C fingerprints from multiple low-concentration biomarkers in complex biological mixtures, a difficult feat to accomplish with existing methods.
Objective
There is a high demand to design approaches capable of tracking the origin of biomarkers in complex biological environments, in the areas of life, environmental, food and forensic sciences. Metabolomics and Fluxomics show great promises towards this aim, and a high potential arises from their combination with Isotopic fingerprinting at natural abundance. The resulting isotopomics approach requires cutting-edge analytical tools, and Nuclear Magnetic Resonance (NMR) is currently the only generic technique giving access to the site-specific isotope content at natural abundance. The detection of very small relative variations between samples originating from different (bio)chemical pathways is possible through 13C isotopic NMR, which can however only be applied to simple and concentrated samples, due to its low sensitivity. Consequently, numerous applications are out of reach. To tackle the current limitations of 13C isotopic analysis, SUMMIT will develop a groundbreaking analytical workflow relying on two of the most powerful NMR methods: dissolution dynamic nuclear polarization and ultrafast 2D NMR. This cutting-edge approach will allow the simultaneous measurement of 13C fingerprints from multiple low-concentrated biomarkers in complex mixtures, which is impossible with existing methods. The high potential of this analytical strategy will be demonstrated on a relevant biological study, the investigation of breast cancer cell metabolism, through applications with gradually increasing risk levels. These approaches will make it possible to identify (i) new biomarkers to discriminate between cell lines expressing different hormonal receptors; (ii) novel potential therapeutic targets from the elucidation of metabolic pathways. Beyond this application, the project will have a high impact on a wide community of academic and industrial researchers, covering unmet needs from life sciences, food industry and forensic analysis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-COG - Consolidator Grant
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2018-COG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
44000 Nantes
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.