Artificial metabolic cells for biomanufacturing of bio-based chiral fine chemicals

Project Information

METACELL

Grant agreement ID: 818089

DOI 10.3030/818089

EC signature date 10 September 2019

Start date 1 January 2020

End date 31 December 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 995 894,00

EU contribution € 1 995 894,00

Coordinated by

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Spain

Publications

Peer reviewed articles (14)

Design of the Enzyme-Carrier Interface to Overcome the O2 and NADH Mass Transfer Limitations of an Immobilized Flavin Oxidase.

Author(s): Ana I. Benítez-Mateos; Ana I. Benítez-Mateos; Christina Huber; Bernd Nidetzky; Juan M. Bolivar; Juan M. Bolivar; Fernando López-Gallego; Fernando López-Gallego
Published in: ACS Applied Materials and Interfaces, Issue 5, 2020, Page(s) 56027-56038, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.0c17568

Selective Coimmobilization of His-Tagged Enzymes on Yttrium-Stabilized Zirconia-Based Membranes for Continuous Asymmetric Bioreductions

Author(s): Andrés-Sanz, D; Diamanti, E; Di Silvo, D; Gurauskis, J; López-Gallego, F.
Published in: ACS Applied Materials and Interfaces, Issue 14-3, 2022, Page(s) 4285-4296, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.1c20738

A heterogeneous chemoenzymatic route toward the continuous transformation of γ-alkynoic acids into γ-hydroxy acids

Author(s): Javier Santiago-Arcos, Daniel Andrés-Sanz, Nicolás Ríos-Lombardía, Susana Carregal-Romero, Desiré di Silvio, Irantzu Llarena, Joaquín García-Álvarez, Javier González-Sabín, Fernando López-Gallego
Published in: Cell Reports Physical Science, Issue 5, 2024, Page(s) 102015, ISSN 2666-3864
Publisher: Elsevier
DOI: 10.1016/j.xcrp.2024.102015

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

Author(s): Alejandro H. Orrego, Daniel Andrés-Sanz, Susana Velasco-Lozano, Mercedes Sanchez-Costa, José Berenguer, José M. Guisan, Javier Rocha-Martin, Fernando López-Gallego
Published in: Catalysis Science & Technology, Issue 11/9, 2021, Page(s) 3217-3230, ISSN 2044-4753
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1cy00268f

Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts

Author(s): Eleftheria Diamanti; Javier Santiago-Arcos; Daniel A. Grajales-Hernández; Nicolette Czarnievicz; Natalia Comino; Irantzu Llarena; Desiré Di Silvio; Aitziber L. Cortajarena; Fernando López-Gallego
Published in: ACS Catalysis, Issue 2, 2021, Page(s) 15051-15067, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c03760

Surpassing Substrate–Enzyme Competition by Compartmentalization

Author(s): Eleftheria Diamanti, Daniel Andrés-Sanz, Alejandro H. Orrego, Susana Carregal-Romero, Fernando López-Gallego
Published in: ACS Catalysis, Issue 13, 2023, Page(s) 11441-11454, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.3c01965

Cell-free biosynthesis of ω-hydroxy acids boosted by a synergistic combination of alcohol dehydrogenases

Author(s): Susana Velasco; Javier Santiago-Arcos; Maria Grazia; Fernando López-Gallego
Published in: ChemSusChem, Issue 1, 2022, ISSN 1864-5631
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.26434/chemrxiv-2021-vgprq

Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis

Author(s): Alba Ledesma-Fernandez, Susana Velasco-Lozano, Javier Santiago-Arcos, Fernando López-Gallego, Aitziber L. Cortajarena
Published in: Nature Communications, Issue 14, 2023, Page(s) 14 - 1 (2587 ), ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-38304-z

Characterization and evaluation of immobilized enzymes for applications in flow reactors

Author(s): Juan M. Bolivar, Fernando López-Gallego
Published in: Current Opinion in Green and Sustainable Chemistry, Issue 25, 2020, Page(s) 100349, ISSN 2452-2236
Publisher: Elsevier
DOI: 10.1016/j.cogsc.2020.04.010

Light-Driven Catalytic Regulation of Enzymes at the Interface with Plasmonic Nanomaterials

Author(s): Heloise Ribeiro de Barros, Fernando López-Gallego, Luis M. Liz-Marzán
Published in: Biochemistry, Issue 60/13, 2021, Page(s) 991-998, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.0c00447

Multienzyme Coimmobilization on Triheterofunctional Supports

Author(s): Javier Santiago-Arcos, Susana Velasco-Lozano, Fernando López-Gallego
Published in: Biomacromolecules, Issue 24, 2023, Page(s) 929-942, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.2c01364

Metal substrate catalysis in the confined space for platinum drug delivery

Author(s): Susana Velasco-Lozano; Silvia Alonso-de Castro; Carlos Sanchez-Cano; Carlos Sanchez-Cano; Ana I. Benítez-Mateos; Fernando López-Gallego; Luca Salassa; Luca Salassa; Luca Salassa
Published in: Chemical Science, Issue 4, 2021, Page(s) 59-67, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sc05151b

Region-Directed Enzyme Immobilization through Engineering Protein Surface with Histidine Clusters

Author(s): Nicoll Zeballos, Natalia Comino, Daniel Andrés-Sanz, Javier Santiago-Arcos, Mikel Azkargorta, Felix Elortza, Eleftheria Diamanti, Fernando López-Gallego
Published in: ACS Applied Materials & Interfaces, Issue 16, 2024, Page(s) 833-846, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.3c15993

ATP‐Independent and Cell‐Free Biosynthesis of β‐Hydroxy Acids Using Vinyl Esters as Smart Substrates

Author(s): Alejandro H. Orrego, Maria Grazia Rubanu, Idania L. López, Daniel Andrés‐Sanz, Guillermo García‐Marquina, German E. Pieslinger, Luca Salassa, Fernando López‐Gallego
Published in: Angewandte Chemie International Edition, Issue 62, 2023, Page(s) 62 - 13 (e202218312), ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202218312