Artificial metabolic cells for biomanufacturing of bio-based chiral fine chemicals

Informazioni relative al progetto

METACELL

ID dell’accordo di sovvenzione: 818089

DOI

10.3030/818089

Progetto chiuso

Data della firma CE 10 Settembre 2019

Data di avvio 1 Gennaio 2020

Data di completamento 31 Dicembre 2025

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 995 894,00

Contributo UE

€ 1 995 894,00

Coordinato da

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Spain

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (23)

Unveiling the spatial rearrangements of exhausted immobilised multi-enzyme systems through cryo-X-ray fluorescence nanoprobe imaging

Autori: Javier Santiago-Arcos, Murielle Salome, Fernando López-Gallego, Carlos Sanchez-Cano
Pubblicato in: Chemical Science, Numero 15, 2024, Pagina/e 20515-20522, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d4sc05136j

Self-Sufficient Heterogeneous Biocatalysis through Boronic Acid-Diol Complexation of Adenylated Cofactors

Autori: Eleftheria Diamanti, Susana Velasco-Lozano, Daniel Grajales-Hernández, Alejandro H. Orrego, Desiré Di Silvio, José María Fraile, Fernando López-Gallego
Pubblicato in: ACS Sustainable Chemistry & Engineering, Numero 11, 2023, Pagina/e 14409-14421, ISSN 2168-0485
Editore: American Chemical Society
DOI: 10.1021/acssuschemeng.3c02958

Design of the Enzyme-Carrier Interface to Overcome the O2 and NADH Mass Transfer Limitations of an Immobilized Flavin Oxidase.

Autori: Ana I. Benítez-Mateos; Ana I. Benítez-Mateos; Christina Huber; Bernd Nidetzky; Juan M. Bolivar; Juan M. Bolivar; Fernando López-Gallego; Fernando López-Gallego
Pubblicato in: ACS Applied Materials and Interfaces, Numero 5, 2020, Pagina/e 56027-56038, ISSN 1944-8244
Editore: American Chemical Society
DOI: 10.1021/acsami.0c17568

Selective Coimmobilization of His-Tagged Enzymes on Yttrium-Stabilized Zirconia-Based Membranes for Continuous Asymmetric Bioreductions

Autori: Andrés-Sanz, D; Diamanti, E; Di Silvo, D; Gurauskis, J; López-Gallego, F.
Pubblicato in: ACS Applied Materials and Interfaces, Numero 14-3, 2022, Pagina/e 4285-4296, ISSN 1944-8244
Editore: American Chemical Society
DOI: 10.1021/acsami.1c20738

A heterogeneous chemoenzymatic route toward the continuous transformation of γ-alkynoic acids into γ-hydroxy acids

Autori: Javier Santiago-Arcos, Daniel Andrés-Sanz, Nicolás Ríos-Lombardía, Susana Carregal-Romero, Desiré di Silvio, Irantzu Llarena, Joaquín García-Álvarez, Javier González-Sabín, Fernando López-Gallego
Pubblicato in: Cell Reports Physical Science, Numero 5, 2024, Pagina/e 102015, ISSN 2666-3864
Editore: Elsevier
DOI: 10.1016/j.xcrp.2024.102015

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

Autori: Alejandro H. Orrego, Daniel Andrés-Sanz, Susana Velasco-Lozano, Mercedes Sanchez-Costa, José Berenguer, José M. Guisan, Javier Rocha-Martin, Fernando López-Gallego
Pubblicato in: Catalysis Science & Technology, Numero 11/9, 2021, Pagina/e 3217-3230, ISSN 2044-4753
Editore: Royal Society of Chemistry
DOI: 10.1039/d1cy00268f

Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts

Autori: Eleftheria Diamanti; Javier Santiago-Arcos; Daniel A. Grajales-Hernández; Nicolette Czarnievicz; Natalia Comino; Irantzu Llarena; Desiré Di Silvio; Aitziber L. Cortajarena; Fernando López-Gallego
Pubblicato in: ACS Catalysis, Numero 2, 2021, Pagina/e 15051-15067, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.1c03760

The Sabatier principle governs the performance of self-sufficient heterogeneous biocatalysts for redox biotransformations

Autori: Eleftheria Diamanti, Ainhoa Oliden-Sánchez, Daniel Grajales-Hernández, Daniel Andrés-Sanz, Rut Fernández-Marín, Daniel Padro, Jesús Ruíz-Cabello, Ronen Zangi, Fernando López-Gallego
Pubblicato in: Cell Reports Physical Science, Numero 6, 2025, Pagina/e 102694, ISSN 2666-3864
Editore: Elsevier BV
DOI: 10.1016/j.xcrp.2025.102694

Loop engineering of enzymes to control their immobilization and ultimately fabricate more efficient heterogeneous biocatalysts

Autori: Nicoll Zeballos, Irene Ginés‐Alcober, Javier Macías‐León, Daniel Andrés‐Sanz, Andrés Manuel González‐Ramírez, Mercedes Sánchez‐Costa, Pedro Merino, Ramón Hurtado‐Guerrero, Fernand
Pubblicato in: Protein Science, Numero 34, 2025, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.70040

Surpassing Substrate–Enzyme Competition by Compartmentalization

Autori: Eleftheria Diamanti, Daniel Andrés-Sanz, Alejandro H. Orrego, Susana Carregal-Romero, Fernando López-Gallego
Pubblicato in: ACS Catalysis, Numero 13, 2023, Pagina/e 11441-11454, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.3c01965

Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids

Autori: Javier Santiago-Arcos, Susana Velasco-Lozano, Eleftheria Diamanti, Daniel A. Grajales-, Ana I. Benítez Mateos, Francesca Paradisi, Fernando López-Gallego
Pubblicato in: Acs Sustainable Chemistry & Engineering, Numero 12, 25, 2024, Pagina/e 9474–9489, ISSN 2168-0485
Editore: American Chemical Society
DOI: 10.21203/rs.3.rs-3644964/v1

Single‐Particle and Single‐Molecule Characterization of Immobilized Enzymes: A Multiscale Path toward Optimizing Heterogeneous Biocatalysts

Autori: Eleftheria Diamanti, Fernando López‐Gallego
Pubblicato in: Angewandte Chemie International Edition, Numero 63, 2024, Pagina/e e202319248, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202319248

Cell-free biosynthesis of ω-hydroxy acids boosted by a synergistic combination of alcohol dehydrogenases

Autori: Susana Velasco; Javier Santiago-Arcos; Maria Grazia; Fernando López-Gallego
Pubblicato in: ChemSusChem, Numero 1, 2022, ISSN 1864-5631
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.26434/chemrxiv-2021-vgprq

Selective chemical recycling of polyhydroxybutyrate into high-value hydroxy acid using the taurine organocatalyst

Autori: Elena Gabirondo, Ainhoa Maiz-Iginitz, Marta Ximenis, Katarzyna Świderek, Daniel Andrés-Sanz, Vicent Moliner, Luis Cabedo, Andrea H. Westlie, Eugene Y.-X. Chen, Daniel Alonso Cerrón-Infantes, Miriam
Pubblicato in: Chemical Science, Numero 16, 2025, Pagina/e 14509-14517, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d5sc02196k

Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis

Autori: Alba Ledesma-Fernandez, Susana Velasco-Lozano, Javier Santiago-Arcos, Fernando López-Gallego, Aitziber L. Cortajarena
Pubblicato in: Nature Communications, Numero 14, 2023, Pagina/e 14 - 1 (2587 ), ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-38304-z

Characterization and evaluation of immobilized enzymes for applications in flow reactors

Autori: Juan M. Bolivar, Fernando López-Gallego
Pubblicato in: Current Opinion in Green and Sustainable Chemistry, Numero 25, 2020, Pagina/e 100349, ISSN 2452-2236
Editore: Elsevier
DOI: 10.1016/j.cogsc.2020.04.010

A Multi-Functional Heterogeneous Biocatalyst for the Oxygen-Free Oxidative Condensation of Primary Alcohols into β-Hydroxy Acids

Autori: Alejandro H. Orrego, Idania L. López, Daniel Andrés-Sanz, Uxoa Fernandez-Pelayo, Maialen Iturralde, Fernando López-Gallego
Pubblicato in: ACS Catalysis, 2025, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.5c06753

Light-Driven Catalytic Regulation of Enzymes at the Interface with Plasmonic Nanomaterials

Autori: Heloise Ribeiro de Barros, Fernando López-Gallego, Luis M. Liz-Marzán
Pubblicato in: Biochemistry, Numero 60/13, 2021, Pagina/e 991-998, ISSN 0006-2960
Editore: American Chemical Society
DOI: 10.1021/acs.biochem.0c00447

Multienzyme Coimmobilization on Triheterofunctional Supports

Autori: Javier Santiago-Arcos, Susana Velasco-Lozano, Fernando López-Gallego
Pubblicato in: Biomacromolecules, Numero 24, 2023, Pagina/e 929-942, ISSN 1525-7797
Editore: American Chemical Society
DOI: 10.1021/acs.biomac.2c01364

Metal substrate catalysis in the confined space for platinum drug delivery

Autori: Susana Velasco-Lozano; Silvia Alonso-de Castro; Carlos Sanchez-Cano; Carlos Sanchez-Cano; Ana I. Benítez-Mateos; Fernando López-Gallego; Luca Salassa; Luca Salassa; Luca Salassa
Pubblicato in: Chemical Science, Numero 4, 2021, Pagina/e 59-67, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d1sc05151b

Microtiter Plate Immobilization Screening for Prototyping Heterogeneous Enzyme Cascades

Autori: Idania L. López, Mercedes Sánchez‐Costa, Alejandro H. Orrego, Nicoll Zeballos, David Roura Padrosa, Fernando López‐Gallego
Pubblicato in: Angewandte Chemie International Edition, Numero 63, 2024, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202407411

Region-Directed Enzyme Immobilization through Engineering Protein Surface with Histidine Clusters

Autori: Nicoll Zeballos, Natalia Comino, Daniel Andrés-Sanz, Javier Santiago-Arcos, Mikel Azkargorta, Felix Elortza, Eleftheria Diamanti, Fernando López-Gallego
Pubblicato in: ACS Applied Materials & Interfaces, Numero 16, 2024, Pagina/e 833-846, ISSN 1944-8244
Editore: American Chemical Society
DOI: 10.1021/acsami.3c15993

ATP‐Independent and Cell‐Free Biosynthesis of β‐Hydroxy Acids Using Vinyl Esters as Smart Substrates

Autori: Alejandro H. Orrego, Maria Grazia Rubanu, Idania L. López, Daniel Andrés‐Sanz, Guillermo García‐Marquina, German E. Pieslinger, Luca Salassa, Fernando López‐Gallego
Pubblicato in: Angewandte Chemie International Edition, Numero 62, 2023, Pagina/e 62 - 13 (e202218312), ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202218312