Multi-Parameter Live-Cell Observation of Biomolecular Processes with Single-Photon Detector Array

Project Information

BrightEyes

Grant agreement ID: 818669

DOI

10.3030/818669

Project closed

EC signature date 4 February 2019

Start date 1 September 2019

End date 28 February 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 861 250,00

EU contribution

€ 1 861 250,00

Coordinated by

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (18)

The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy

Author(s): Alessandro Rossetta, Eli Slenders, Mattia Donato, Sabrina Zappone, Francesco Fersini, Martina Bruno, Francesco Diotalevi, Luca Lanzanò, Sami Koho, Giorgio Tortarolo, Andrea Barberis, Marco Crepaldi, Eleonora Perego, Giuseppe Vicidomini
Published in: Nature Communications, Issue 13, 2022, Page(s) 7406, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-35064-0

M6A reduction relieves FUS-associated ALS granules

Author(s): Gaia Di Timoteo, Andrea Giuliani, Adriano Setti, Martina C. Biagi, Michela Lisi, Tiziana Santini, Alessia Grandioso, Davide Mariani, Francesco Castagnetti, Eleonora Perego, Sabrina Zappone, Serena Lattante, Mario Sabatelli, Dante Rotili, Giuseppe Vicidomini, Irene Bozzoni
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-49416-5

4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector

Author(s): Andrea Bucci, Giorgio Tortarolo, Marcus Oliver Held, Luca Bega, Eleonora Perego, Francesco Castagnetti, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-50512-9

Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy

Author(s): Eli Slenders, Eleonora Perego, Mauro Buttafava, Giorgio Tortarolo, Enrico Conca, Sabrina Zappone, Agnieszka Pierzynska-Mach, Federica Villa, Enrica Maria Petrini, Andrea Barberis, Alberto Tosi, Giuseppe Vicidomini
Published in: Biophysical Report, Issue 1(2), 2021, Page(s) 100025, ISSN 2667-0747
Publisher: Cell Press
DOI: 10.1016/j.bpr.2021.100025

The role of RNA in the nanoscale organization of α-synuclein phase separation

Author(s): Sabrina Zappone, Eleonora Perego, Jakob Rupert, Elsa Zacco, Eli Slenders, Gian Gaetano Tartaglia, Giuseppe Vicidomini
Published in: NAR Molecular Medicine, Issue 2, 2025, ISSN 2976-856X
Publisher: Oxford University Press (OUP)
DOI: 10.1093/narmme/ugaf012

Compact and effective photon-resolved image scanning microscope

Author(s): Giorgio Tortarolo, Alessandro Zunino, Simonluca Piazza, Mattia Donato, Sabrina Zappone, Agnieszka Pierzyńska-Mach, Marco Castello, Giuseppe Vicidomini
Published in: Advanced Photonics, Issue 6(1), 2024, Page(s) 016003, ISSN 2577-5421
Publisher: SPIE and Chinese Laser Press
DOI: 10.1117/1.ap.6.1.016003

Single-photon microscopy to study biomolecular condensates

Author(s): Eleonora Perego, Sabrina Zappone, Francesco Castagnetti, Davide Mariani, Erika Vitiello, Jakob Rupert, Elsa Zacco, Gian Gaetano Tartaglia, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini
Published in: Nature Communications, Issue 14, 2023, Page(s) 8224, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-43969-7

ALS-associated FUS mutation reshapes the RNA and protein composition of stress granules

Author(s): Davide Mariani, Adriano Setti, Francesco Castagnetti, Erika Vitiello, Lorenzo Stufera Mecarelli, Gaia Di Timoteo, Andrea Giuliani, Angelo D’Angelo, Tiziana Santini, Eleonora Perego, Sabrina Zappone, Nara Liessi, Andrea Armirotti, Giuseppe Vicidomini, Irene Bozzoni
Published in: Nucleic Acids Research, Issue 52, 2024, Page(s) 13269-13289, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkae942

Reconstructing the image scanning microscopy dataset: an inverse problem

Author(s): Alessandro Zunino, Marco Castello, Giuseppe Vicidomini
Published in: Inverse Problem, Issue 39(6), 2023, Page(s) 064004, ISSN 0266-5611
Publisher: Institute of Physics Publishing
DOI: 10.1088/1361-6420/accdc5

Two-photon image-scanning microscopy with SPAD array and blind image reconstruction

Author(s): Sami V. Koho, Eli Slenders, Giorgio Tortarolo, Marco Castello, Mauro Buttafava, Federica Villa, Elena Tcarenkova, Marcel Ameloot, Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Alberto Tosi, Giuseppe Vicidomini
Published in: Biomedical Optics Express, Issue 11/6, 2020, Page(s) 2905, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.374398

Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector

Author(s): Eli Slenders, Marco Castello, Mauro Buttafava, Federica Villa, Alberto Tosi, Luca Lanzanò, Sami Valtteri Koho, Giuseppe Vicidomini
Published in: Light: Science & Applications, Issue 10/1, 2021, Page(s) 31, ISSN 2047-7538
Publisher: Springer Nature
DOI: 10.1038/s41377-021-00475-z

ISM-FLUX: MINFLUX with an array detector

Author(s): Eli Slenders, Giuseppe Vicidomini
Published in: Physical Review Research, Issue 5, 2023, ISSN 2643-1564
Publisher: American Physical Society (APS)
DOI: 10.1103/physrevresearch.5.023033

Open-source 3D active sample stabilization for fluorescence microscopy

Author(s): Sanket Patil, Giuseppe Vicidomini, Eli Slenders
Published in: Biophysical Reports, Issue 5, 2025, Page(s) 100208, ISSN 2667-0747
Publisher: Elsevier BV
DOI: 10.1016/j.bpr.2025.100208

Image scanning microscopy reconstruction by autocorrelation inversion

Author(s): Daniele Ancora, Alessandro Zunino, Giuseppe Vicidomini, Alvaro H Crevenna
Published in: Journal of Physics: Photonics, Issue 6, 2024, Page(s) 045003, ISSN 2515-7647
Publisher: IOP Publishing
DOI: 10.1088/2515-7647/ad68dd

BrightEyes-MCS: a control software for multichannelscanning microscopy

Author(s): Mattia Donato, Eli Slenders, Alessandro Zunino, Luca Bega, Giuseppe Vicidomini
Published in: Journal of Open Source Software, Issue 9, 2024, Page(s) 7125, ISSN 2475-9066
Publisher: The Open Journal
DOI: 10.21105/joss.07125

Wavefront estimation through structured detection in laser scanning microscopy

Author(s): Francesco Fersini, Alessandro Zunino, Pietro Morerio, Francesca Baldini, Alberto Diaspro, Martin J. Booth, Alessio Del Bue, Giuseppe Vicidomini
Published in: Biomedical Optics Express, Issue 16, 2025, Page(s) 2135, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.559899

SPAD-based asynchronous-readout array detectors for image-scanning microscopy

Author(s): Mauro Buttafava, Federica Villa, Marco Castello, Giorgio Tortarolo, Enrico Conca, Mirko Sanzaro, Simonluca Piazza, Paolo Bianchini, Alberto Diaspro, Franco Zappa, Giuseppe Vicidomini, Alberto Tosi
Published in: Optica, Issue 7/7, 2020, Page(s) 755, ISSN 2334-2536
Publisher: The Optical Society
DOI: 10.1364/optica.391726

Focus image scanning microscopy for sharp and gentle super-resolved microscopy

Author(s): Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J. R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini
Published in: Nature Communications, Issue 13, 2022, Page(s) 7723, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-35333-y

Other (14)

ALS-associated FUS mutation reshapes the RNA and protein composition of Stress Granules

Author(s): Davide Mariani, Adriano Setti, Francesco Castagnetti, Erika Vitiello, Lorenzo Stufera Mecarelli, Gaia di Timoteo, Andrea Giuliani, Eleonora Perego, Sabrina Zappone, Nara Liessi, Andrea Armirotti, Giuseppe Vicidomini, Irene Bozzoni
Published in: bioRxiv, 2023
Publisher: Cold Spring Harbour
DOI: 10.1101/2023.09.11.557245

ISM-FLUX: single-step MINFLUX with an array detector

Author(s): Eli Slenders, Giuseppe Vicidomini
Published in: bioRxiv, 2022, Page(s) 2022.04.19.488747
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.04.19.488747

Structured Detection for Simultaneous Super-Resolution and Optical Sectioning in Laser Scanning Microscopy

Author(s): Alessandro Zunino, Giacomo Garrè, Eleonora Perego, Sabrina Zappone, Mattia Donato, Giuseppe Vicidomini
Published in: arXiv, 2024
Publisher: Cornell University
DOI: 10.48550/arxiv.2406.12542

Array Detection Enables Large Localization Range for Simple and Robust MINFLUX

Author(s): Eli Slenders, Sanket Patil, Marcus Oliver Held, Alessandro Zunino, Giuseppe Vicidomini
Published in: bioRxiv, 2024
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.07.08.602588

Open-Source 3<i>D</i>Active Sample Stabilization for Fluorescence Microscopy

Author(s): Sanket Patil, Giuseppe Vicidomini, Eli Slenders
Published in: bioRxiv, 2025
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.01.10.631718

A Compact and Effective Photon-Resolved Image Scanning Microscope

Author(s): Giorgio Tortarolo, Alessandro Zunino, Simonluca Piazza, Mattia Donato, Sabrina Zappone, Agnieszka Pierzyńska-Mach, Marco Castello, Giuseppe Vicidomini
Published in: bioRxiv, 2023
Publisher: Cold Spring Harbour
DOI: 10.1101/2023.07.28.549477

The role of RNA in the nanoscale organization of alpha-synuclein phase separation

Author(s): Sabrina Zappone, Eleonora Perego, Jakob Rupert, Elsa Zacco, Eli Slenders, Gian Gaetano Tartaglia, Giuseppe Vicidomini
Published in: bioRxiv, 2024
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.09.03.610813

Focus-ISM for Sharp and Gentle Super-Resolved Microscopy

Author(s): Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J.R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, and Giuseppe Vicidomini
Published in: bioRxiv, 2022, Page(s) 2022.04.28.489892
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.04.28.489892

M6A reduction relieves FUS-associated ALS granules

Author(s): Gaia Di Timoteo, Andrea Giuliani, Adriano Setti, Martina C. Biagi, Michela Lisi, Alessia Grandioso, Davide Mariani, Francesco Castagnetti, Eleonora Perego, Sabrina Zappone, Giuseppe Vicidomini, Dante Rotili, Mario Sabatelli, Serena Lattante, Irene Bozzoni
Published in: bioRxiv, 2023
Publisher: Cold Spring Harbour
DOI: 10.1101/2023.10.25.563954

Wavefront estimation through structured detection in laser scanning microscopy

Author(s): Francesco Fersini, Alessandro Zunino, Pietro Morerio, Francesca Baldini, Martin J. Booth, Alessio Del Bue, Giuseppe Vicidomini
Published in: bioRxiv, 2025
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.12.31.630574

4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector

Author(s): Andrea Bucci, Giorgio Tortarolo, Marcus Oliver Held, Luca Bega, Eleonora Perego, Francesco Castagnetti, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini
Published in: bioRxiv, 2023
Publisher: Cold Spring Harbour Laboratory
DOI: 10.1101/2023.08.25.554867

Reconstructing the Image Scanning Microscopy Dataset: an Inverse Problem

Author(s): Alessandro Zunino, Marco Castello, Giuseppe Vicidomini
Published in: arXiv, Issue arXiv:2211.12510, 2022
Publisher: Cornell University
DOI: 10.48550/arxiv.2211.12510

The BrightEyes-TTM: an Open-Source Time-Tagging Module for Single-Photon Microscopy

Author(s): Alessandro Rossetta, Eli Slenders, Mattia Donato, Eleonora Perego, Francesco Diotalevi, Luca Lanzanó, Sami Koho, Giorgio Tortarolo, Marco Crepaldi, Giuseppe Vicidomini
Published in: bioRxiv, 2021, Page(s) 2021.10.11.463950
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.10.11.463950

Content-enriched fluorescence lifetime fluctuation spectroscopy to study bio-molecular condensate formation

Author(s): Eleonora Perego, Sabrina Zappone, Francesco Castagnetti, Davide Mariani, Erika Vitiello, Jakob Rupert, Elsa Zacco, Gian Gaetano Tartaglia, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini
Published in: bioRxiv, 2023, Page(s) 2023.06.09.544221, ISSN 2692-8205
Publisher: Cold Spring Harbour Laboratory
DOI: 10.1101/2023.06.09.544221

Non-peer reviewed articles (1)

Open-source tools enable accessible and advanced image scanning microscopy data analysis

Author(s): Alessandro Zunino, Eli Slenders, Francesco Fersini, Andrea Bucci, Mattia Donato, Giuseppe Vicidomini
Published in: Nature Photonics, Issue 17, 2023, Page(s) 457–458, ISSN 1749-4885
Publisher: Nature Pub. Group
DOI: 10.1038/s41566-023-01216-x