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Programmable nucleic acid toolkits for cell-free diagnostics and genetically encoded biosensing

Project description

Nucleic acid nanotechnology for the creation of cell-free point-of-care diagnostics

The biological complexity of tumours and the diversity of diagnostic biomarkers require the development of innovative analytical tools for the detection of multiple targets in a sensitive, specific and low-cost way to allow real-time monitoring of disease progression and therapeutic effects. The goal of the EU-funded PRO-TOOLKITS project is to introduce innovative cell-free point-of-care diagnostic kits and genetically manipulated biosensing tools. The researchers will develop rationally designed programmable nucleic acid modules that respond to a wide range of molecular markers and environmental stimuli. The project's work will lead to significant breakthroughs in the fields of in vitro diagnostics, biosensing and synthetic biology.

Objective

WHY: The biological complexity of tumours and the large diversity of diagnostic biomarkers call for the development of innovative analytical tools that can detect multiple targets in a sensitive, specific and low-cost way and allow real-time monitoring of disease pathways and therapeutic effects. To provide such transformative tools creative thinking, innovative approach and the exploration of new research avenues that span different disciplines is necessary.

WHAT: The goal of the PRO-TOOLKITS project is to address this need by developing innovative cell-free point of care diagnostic kits and genetically encodable biosensing tools.

HOW: I oriented my independent career as a P.I. towards the design and development of synthetic nucleic acid-based nanodevices and nanomachines. With the help of an ERC Starting Grant I made ground-breaking contributions in the field of nucleic acid Nanotechnology. Motivated by these advancements I propose to challenge my know-how and expertise to explore new research avenues that will open exciting possibilities in biosensing applications. The key, ground-breaking IDEA underlying this project is to take advantage of my expertise and harness the advantageous features of RNA synthetic modules that can translate the expression of proteins in controlled in-vitro cell-free systems and can be also genetically encoded in living organisms and function inside the cells. I will develop rationally designed programmable nucleic acid modules that respond to a wide range of molecular markers and environmental stimuli through innovative nature-inspired mechanisms and that can be orthogonally wired to provide cell-free diagnostic kits and genetically encoded live-cell biosensing tools. The project will provide transformative approaches, methods and tools that will represent a genuine break-through in the fields of in-vitro diagnostics, biosensing and synthetic biology.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-COG - Consolidator Grant

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2018-COG

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Host institution

UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 999 375,00
Address
VIA CRACOVIA 50
00133 Roma
Italy

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Region
Centro (IT) Lazio Roma
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 999 375,00

Beneficiaries (1)

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