Descripción del proyecto
Una nantotecnología de ácidos nucleicos para la creación de diagnósticos sin células en el punto de atención
La complejidad biológica de los tumores y la diversidad de biomarcadores de diagnóstico requieren el desarrollo de herramientas analíticas innovadoras para la detección de múltiples dianas de una forma sensible, específica y con bajo coste a fin de permitir el seguimiento en tiempo real de la progresión de enfermedades y los efectos terapéuticos. El objetivo del proyecto financiado con fondos europeos PRO-TOOLKITS es introducir unos innovadores kits de diagnóstico sin células en el punto de atención y herramientas de biodetección manipuladas genéticamente. Los investigadores del proyecto desarrollarán unas moléculas de ácidos nucleicos programables diseñadas de forma racional que respondan a una amplia variedad de marcadores moleculares y estímulos ambientales. El trabajo del proyecto dará lugar a avances significativos en los campos del diagnóstico «in vitro», la biodetección y la biología sintética.
Objetivo
WHY: The biological complexity of tumours and the large diversity of diagnostic biomarkers call for the development of innovative analytical tools that can detect multiple targets in a sensitive, specific and low-cost way and allow real-time monitoring of disease pathways and therapeutic effects. To provide such transformative tools creative thinking, innovative approach and the exploration of new research avenues that span different disciplines is necessary.
WHAT: The goal of the PRO-TOOLKITS project is to address this need by developing innovative cell-free point of care diagnostic kits and genetically encodable biosensing tools.
HOW: I oriented my independent career as a P.I. towards the design and development of synthetic nucleic acid-based nanodevices and nanomachines. With the help of an ERC Starting Grant I made ground-breaking contributions in the field of nucleic acid Nanotechnology. Motivated by these advancements I propose to challenge my know-how and expertise to explore new research avenues that will open exciting possibilities in biosensing applications. The key, ground-breaking IDEA underlying this project is to take advantage of my expertise and harness the advantageous features of RNA synthetic modules that can translate the expression of proteins in controlled in-vitro cell-free systems and can be also genetically encoded in living organisms and function inside the cells. I will develop rationally designed programmable nucleic acid modules that respond to a wide range of molecular markers and environmental stimuli through innovative nature-inspired mechanisms and that can be orthogonally wired to provide cell-free diagnostic kits and genetically encoded live-cell biosensing tools. The project will provide transformative approaches, methods and tools that will represent a genuine break-through in the fields of in-vitro diagnostics, biosensing and synthetic biology.
Ámbito científico
Palabras clave
Programa(s)
Régimen de financiación
ERC-COG - Consolidator GrantInstitución de acogida
00133 Roma
Italia