Biocatalysis for Sustainable Chemistry – Understanding Oxidation/Reduction of Small Molecules by Redox Metalloenzymes via a Suite of Steady State and Transient Infrared Electrochemical Methods

Project Information

BiocatSusChem

Grant agreement ID: 819580

DOI

10.3030/819580

Project closed

EC signature date 11 February 2019

Start date 1 March 2019

End date 28 February 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 997 286,00

EU contribution

€ 1 997 286,00

Coordinated by

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Other (2)

Cofactor-free biocatalytic hydrogenation of nitro compounds for synthesis of amines

Author(s): Daria Sokolova, Tara Lurshay, Jack Rowbotham, Georgia Stonadge, Holly Reeve, Sarah Cleary, Tim Sudmeier, Kylie Vincent
Published in: ChemXRIV, 2024
Publisher: American Chemical Society (ACS)
DOI: 10.26434/chemrxiv-2024-vqfc1

Glutamate “flick” enables proton tunneling during fast redox biocatalysis

Author(s): Stephen B. Carr, Wangzhe Li, Kin Long Wong, Rhiannon M. Evans, Sophie E. T. Kendall-Price, Kylie A. Vincent, Philip A. Ash
Published in: ChemXRIV, 2024
Publisher: American Chemical Society (ACS)
DOI: 10.26434/chemrxiv-2024-mn36l

Peer reviewed articles (18)

The 2022 solar fuels roadmap

Author(s): Gideon Segev; Jakob Kibsgaard; Christopher Hahn; Zhichuan J Xu; Wen-Hui (Sophia) Cheng; Todd G Deutsch; Chengxiang Xiang; Jenny Z Zhang; Leif Hammarström; Daniel G Nocera; Adam Z Weber; Peter Agbo; Takashi Hisatomi; Frank E Osterloh; Kazunari Domen; Fatwa F Abdi; Sophia Haussener; Daniel J Miller; Shane Ardo; Paul C McIntyre; Thomas Hannappel; Shu Hu; Harry Atwater; John M Gregoire; Mehmed Z Erte
Published in: Journal of Physics D: Applied Physics, Issue Vol 55, Iss 32, 2022, Page(s) 323003, ISSN 1361-6463
Publisher: IOPScience
DOI: 10.1088/1361-6463/ac6f97

H2-Driven Reduction of Flavin by Hydrogenase Enables Cleaner Operation of Nitroreductases for Nitro-Group to Amine Reductions

Author(s): Miguel A. Ramirez, Shiny Joseph Srinivasan, Sarah E. Cleary, Peter M. T. Todd, Holly A. Reeve, Kylie A. Vincent
Published in: Frontiers in Catalysis, Issue Vol 2, 2022, Page(s) https://doi.org/10.3389/fctls.2022.906694, ISSN 2673-7841
Publisher: Frontiers
DOI: 10.3389/fctls.2022.906694

E. coli Nickel‐Iron Hydrogenase 1 Catalyses Non‐native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene‐reductases**

Author(s): Shiny Joseph Srinivasan; Sarah E. Cleary; Miguel A. Ramirez; Holly A. Reeve; Caroline E. Paul; Kylie A. Vincent
Published in: Angewandte Chemie (International Ed. in English), Issue Vol 60, 2021, Page(s) 13824-13828, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202101186

Biocatalytic reductive amination as a route to isotopically labelled amino acids suitable for analysis of large proteins by NMR

Author(s): Jack S. Rowbotham, Jake H. Nicholson, Miguel A. Ramirez, Kouji Urata, Peter M. T. Todd, Gogulan Karunanithy, Lars Lauterbach, Holly A. Reeve, Andrew J. Baldwin, Kylie A. Vincent
Published in: Chemical Science, Issue 14, 2024, Page(s) 12160-12165, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3sc01718d

The crystalline state as a dynamic system: IR microspectroscopy under electrochemical control for a [NiFe] hydrogenase

Author(s): Philip A. Ash; Sophie E. T. Kendall-Price; Rhiannon M. Evans; Stephen B. Carr; Amelia R. Brasnett; Simone Morra; Jack S. Rowbotham; Ricardo Hidalgo; Adam J. Healy; Gianfelice Cinque; Mark D. Frogley; Fraser A. Armstrong; Kylie A. Vincent
Published in: Chemical Science, Issue Vol 12, 2021, Page(s) 12959-12970, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sc01734a

Controlled Biocatalytic Synthesis of a Metal Nanoparticle‐Enzyme Hybrid: Demonstration for Catalytic H₂‐driven NADH Recycling

Author(s): Lucy B. F. Browne, Tim Sudmeier, Maya A. Landis, Christopher S. Allen, Kylie A. Vincent
Published in: Angewandte Chemie, Issue 136, 2024, Page(s) e202404024, ISSN 0044-8249
Publisher: Wiley
DOI: 10.1002/ange.202404024

Replacing a Cysteine Ligand by Selenocysteine in a [NiFe]-Hydrogenase Unlocks Hydrogen Production Activity and Addresses the Role of Concerted Proton-Coupled Electron Transfer in Electrocatalytic Reversibility

Author(s): Rhiannon M. Evans, Natalie Krahn, Joshua Weiss, Kylie A. Vincent, Dieter Söll, Fraser A. Armstrong
Published in: Journal of the American Chemical Society, Issue 146, 2024, Page(s) 16971-16976, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.4c03489

Stability of the H-cluster under whole-cell conditions—formation of an Htrans-like state and its reactivity towards oxygen

Author(s): Marco Lorenzi; Pierre Ceccaldi; Patricia Rodríguez-Maciá; Holly Jayne Redman; Afridi Zamader; James A. Birrell; Livia S. Mészáros; Gustav Berggren
Published in: Journal of Biological Inorganic Chemistry, Issue 1, 2022, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-022-01928-5

Exploiting hydrogenases for biocatalytic hydrogenations

Author(s): Daria Sokolova, Kylie A. Vincent
Published in: Chemical Communications, Issue 60, 2024, Page(s) 13667-13677, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d4cc04525d

The Nonphysiological Reductant Sodium Dithionite and [FeFe] Hydrogenase: Influence on the Enzyme Mechanism.

Author(s): Maria Alessandra Martini; Olaf Rüdiger; Nina Breuer; Birgit Nöring; Serena DeBeer; Patricia Rodríguez-Maciá; James A. Birrell
Published in: Journal of the American Chemical Society, Issue 1, 2021, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.1c07322

Binuclear Lanthanide Complexes as Magnetic Resonance and Optical Imaging Probes for Redox Sensing

Author(s): Charlie H. Simms, Daniel Kovacs, Lina Hacker, Euan T. Sarson, Daria Sokolova, Kirsten E. Christensen, Alexandr Khrapichev, Louise A. W. Martin, Kylie Vincent, Stuart J. Conway, Ester M. Hammond, Matthew J. Langton, Stephen Faulkner
Published in: Chemistry – A European Journal, Issue 31, 2025, Page(s) e202404748, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202404748

Electrochemical experiments define potentials associated with binding of substrates and inhibitors to nitrogenase MoFe protein

Author(s): Ting Chen; Philip A. Ash; Lance C. Seefeldt; Kylie A. Vincent
Published in: Faraday Discussions, Issue 243, 2023, Page(s) 270-286, ISSN 1364-5498
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2fd00170e

Riboflavin with H₂‐Driven or Electrochemical Recycling: A Cheap Cofactor System for Supporting Biocatalytic Alkene Reduction

Author(s): Lucy B. F. Browne, Charlotte C. Hancox, Sofia M. Helin, Peter M. T. Todd, Kylie A. Vincent
Published in: ChemCatChem, Issue 17, 2025, ISSN 1867-3880
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.202401578

Cyanophenylalanine as an Infrared Probe for Iron–Sulfur Cluster Redox State in Multicenter Metalloenzymes

Author(s): Zehui Duan, Jiaao Wei, Stephen B. Carr, Miguel Ramirez, Rhiannon M. Evans, Philip A. Ash, Patricia Rodriguez‐Macia, Amit Sachdeva, Kylie A. Vincent
Published in: ChemBioChem, Issue doi:10.1002/cbic.202500251, 2025, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202500251

Electrochemical control of [FeFe]-hydrogenase single crystals reveals complex redox populations at the catalytic site

Author(s): Simone Morra; Jifu Duan; Martin Winkler; Philip A. Ash; Thomas Happe; Kylie A. Vincent
Published in: Dalton Transactions, Issue Vol 50, 2021, Page(s) 12655-12663, ISSN 1477-9226
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1dt02219a

Selective hydrogenation of nitro compounds to amines by coupled redox reactions over a heterogeneous biocatalyst

Author(s): Daria Sokolova, Tara C. Lurshay, Jack S. Rowbotham, Georgia Stonadge, Holly A. Reeve, Sarah E. Cleary, Tim Sudmeier, Kylie A. Vincent
Published in: Nature Communications, Issue 15, 2024, Page(s) 7297, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-51531-2

A Beginner’s Guide to Thermodynamic Modelling of [FeFe] Hydrogenase

Author(s): James A. Birrell; Patricia Rodríguez-Maciá; Adrian Hery-Barranco
Published in: Catalysts, Issue Vol 11, 2021, Page(s) 238, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal11020238

Reversible Enzymatic Switching of the Oxidation State of a Eu ^III/II Complex Controls Relaxivity

Author(s): Euan T. Sarson, Saul M. Cooper, Adam C. Sedgwick, Amy-Grace Berger, Sophie A. Twigger, Ester M. Hammond, Kylie A. Vincent, Stephen Faulkner
Published in: Journal of the American Chemical Society, Issue 147, 2025, Page(s) 38992-38997, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.5c13447

Intellectual Property Rights

Patent (2)

METHOD OF REDUCING AND RECYCLING OXIDIZED FLAVIN COFACTORS

Application/Publication number: 20 21051000
Date: 2021-04-23
Applicant(s): THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

METHOD OF REDUCING AND RECYCLING OXIDIZED FLAVIN COFACTORS

Application/Publication number: 20 21051000
Date: 2021-04-23
Applicant(s): THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD