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EBiSC2 – A sustainable European Bank for induced pluripotent Stem Cells

Deliverables

EBiSC2 regime for ethical and legal requirements

Guidelines describing all standard procedures set up to ensure compliance with ethical and legal requirements for the unlimited use and distribution of banked iPSC lines to promote best practice and international harmonisation

Integration concepts and banking workflows for iPSC lines from external consortia

Report describing the process enabling the integration of already existing and newly established iPSC assets into the EBiSC2 bank.

Impact of biocompatible materials on iPSC-derived cardiac cell types functionality evaluated

Report evaluating the impact of biocompatible materials on iPSCderived cardiac cell types functionality

Hepatocyte modelling PoC completed

PoC models completed and data transferred to WP5 gene editing tools established and EFPIA motivated cell line generation completed and transferred to WP3

Established workflows for certified re-banking of iPSC lines and derivative stocks

Plan describing the generation of the initial stock and appropriate strategies and workflows for the rebanking of highindemand iPSC lines and iPSCderived cells as well as externally submitted assets

Protocols for cryopreservation of bulk quantities of iPSC and iPSC-derived cells established

Cryopreservation protocols for bulk quantities of iPSC and iPSCderived cells

Assessment of drug efficacy conducted

Report on the assessment of drug efficacy and maturation

Data management handbook

Report incl SOPs describing the concepts for data security backup protection and access regulations to provide safe secure and uptodate technical hardware structures

Protocol and workflow for optimized hepatocyte differentiation established

PoC models completed and data transferred to WP5 gene editing tools established and EFPIA motivated cell line generation completed and transferred to WP3

Enhanced protocols for large-scale iPSC differentiation established

Set of refined large-scale iPSC differentiation protocols

QC criteria for progenitor cell types identified

QC panel identified and defined for progenitor cells. Panel identification transferred to WP3.

Automated data validation established

Automated IT workflow replaces the initial manual system while ensuring low maintenance, low risk and high automation

First induced differentiated GE line generated, characterised and banked for accelerated differentiation and maturation of CNS cell types

GE lines established, PoC models completed, data transferred to WP5 and EFPIA motivated cell line generation completed and transferred to WP3

Standard API established for data integration

Definition and implementation of data standards and codification for automated data exchange and mapping

Fully operable EBiSC2 bank established

All required operations have been performed to allow operations of the EBiSC2 bank after Month 3.

At least three EFPIA-driven cell line commissioning projects completed

Outcome of at least three EFPIA-driven cell line commissioning projects carried out.

Establishment of new branding and trademark registration

Presentation of the EBiSC2 branding and registration as trademark to ensure the legal framework

Portal and interface implemented

Portal and website redesigned and launched

Publications

SOX9-induced Generation of Functional Astrocytes Supporting Neuronal Maturation in an All-human System

Author(s): Katrien Neyrinck, Johanna Van Den Daele, Tim Vervliet, Jonathan De Smedt, Keimpe Wierda, Melissa Nijs, Tom Vanbokhoven, Astrid D’hondt, Mélanie Planque, Sarah-Maria Fendt, Pei-Yu Shih, Frederik Seibt, Juan Pita Almenar, Mohamed Kreir, Devesh Kumar, Vania Broccoli, Geert Bultynck, Andreas Ebneth, Alfredo Cabrera-Socorro, Catherine Verfaillie
Published in: Stem Cell Reviews and Reports, 2021, ISSN 2629-3269
Publisher: Springer
DOI: 10.1007/s12015-021-10179-x

The EBiSC iPSC bank for disease studies

Author(s): Rachel Steeg, Julia C. Neubauer, Sabine C. Müller, Andreas Ebneth, Heiko Zimmermann
Published in: Stem Cell Research, Issue 49, 2020, Page(s) 102034, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.102034

EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines

Author(s): Rachel Steeg, Sabine C. Mueller, Nancy Mah, Bjørn Holst, Alfredo Cabrera-Socorro, Glyn N. Stacey, Paul A. De Sousa, Aidan Courtney, Heiko Zimmermann
Published in: Stem Cell Reports, Issue 16/8, 2021, Page(s) 1853-1867, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2021.07.009

Corrigendum to “Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line” [Stem Cell Res. 34/1873–5061 (2019) 101349–55]

Author(s): Benjamin Schmid, Kennie R. Prehn, Natakarn Nimsanor, Blanca Irene Aldana Garcia, Ulla Poulsen, Ida Jørring, Mikkel A. Rasmussen, Christian Clausen, Ulrike A. Mau-Holzmann, Sarayu Ramakrishna, Ravi Muddashetty, Rachel Steeg, Kevin Bruce, Peter Mackintosh, Andreas Ebneth, Bjørn Holst, Alfredo Cabrera-Socorro
Published in: Stem Cell Research, Issue 48, 2020, Page(s) 102005, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.102005

Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line

Author(s): Benjamin Schmid, Kennie R. Prehn, Natakarn Nimsanor, Blanca Irene Aldana Garcia, Ulla Poulsen, Ida Jørring, Mikkel A. Rasmussen, Christian Clausen, Ulrike A. Mau-Holzmann, Sarayu Ramakrishna, Ravi Muddashetty, Rachel Steeg, Kevin Bruce, Peter Mackintosh, Andreas Ebneth, Bjørn Holst, Alfredo Cabrera-Socorro
Published in: Stem Cell Research, Issue 34, 2019, Page(s) 101349, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2018.11.010

Human iPSC-derived hepatocytes in 2D and 3D suspension culture for cryopreservation and in vitro toxicity studies

Author(s): Saskia Altmaier, Ina Meiser, Emilie Lemesre, Benjamin Chanrion, Rachel Steeg, Lidia Elena Leonte, Bjørn Holst, Boye Schnack Nielsen, Christian Clausen, Katharina Schmidt, Anne Marie Vinggaard, Heiko Zimmermann, Julia Christiane Neubauer, Mikkel Aabech Rasmussen
Published in: Reproductive Toxicology, Issue 111, 2022, Page(s) 68-80, ISSN 0890-6238
Publisher: Elsevier BV
DOI: 10.1016/j.reprotox.2022.05.005

Development and implementation of large-scale quality control for the European bank for induced Pluripotent Stem Cells

Author(s): Orla O'Shea, Rachel Steeg, Charlotte Chapman, Peter Mackintosh, Glyn N Stacey
Published in: Stem Cell Research, Issue 45, 2020, Page(s) 101773, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.101773

Development of a fully human assay combining NGN2-inducible neurons co-cultured with iPSC-derived astrocytes amenable for electrophysiological studies

Author(s): Pei-Yu Shih, Mohamed Kreir, Devesh Kumar, Frederik Seibt, Francisco Pestana, Benjamin Schmid, Bjørn Holst, Christian Clausen, Rachel Steeg, Benjamin Fischer, Juan Pita-Almenar, Andreas Ebneth, Alfredo Cabrera-Socorro
Published in: Stem Cell Research, Issue 54, 2021, Page(s) 102386, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2021.102386

Scalable expansion of iPSC and their derivatives across multiple lineages

Author(s): Chee Keong Kwok, Isabelle Sebastien, Krithika Hariharan, Ina Meiser, Jeanette Wihan, Saskia Altmaier, Isabell Karnatz, Dominic Bauer, Benjamin Fischer, Alexander Feile, Alfredo Cabrera-Socorro, Mikkel Rasmussen, Bjørn Holst, Julia C. Neubauer, Christian Clausen, Catherine Verfaillie, Andreas Ebneth, Mattias Hansson, Rachel Steeg, Heiko Zimmermann
Published in: Reproductive Toxicology, Issue 112, 2022, Page(s) 23-35, ISSN 0890-6238
Publisher: Elsevier BV
DOI: 10.1016/j.reprotox.2022.05.007

Access to stem cell data and registration of pluripotent cell lines: The Human Pluripotent Stem Cell Registry (hPSCreg)

Author(s): Nancy Mah, Stefanie Seltmann, Begoña Aran, Rachel Steeg, Johannes Dewender, Nils Bultjer, Anna Veiga, Glyn N. Stacey, Andreas Kurtz
Published in: Stem Cell Research, Issue 47, 2020, Page(s) 101887, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.101887

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

Author(s): Jeanette Wihan
Published in: Journal of Visualized Experiments, Issue 6, 2023, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/65085

Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus

Author(s): Benjamin Schmid, Bjørn Holst, Ulla Poulsen, Ida Jørring, Christian Clausen, M. Rasmussen, Ulrike A. Mau-Holzmann, Rachel Steeg, Hugh Nuthall, A. Ebneth, A. Cabrera-Socorro
Published in: Stem Cell Research, Issue 52, 2021, Page(s) 102240, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2021.102240

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