Risultati finali Documents, reports (11) EBiSC2 regime for ethical and legal requirements Guidelines describing all standard procedures set up to ensure compliance with ethical and legal requirements for the unlimited use and distribution of banked iPSC lines to promote best practice and international harmonisation Integration concepts and banking workflows for iPSC lines from external consortia Report describing the process enabling the integration of already existing and newly established iPSC assets into the EBiSC2 bank. Impact of biocompatible materials on iPSC-derived cardiac cell types functionality evaluated Report evaluating the impact of biocompatible materials on iPSCderived cardiac cell types functionality Hepatocyte modelling PoC completed PoC models completed and data transferred to WP5 gene editing tools established and EFPIA motivated cell line generation completed and transferred to WP3 Established workflows for certified re-banking of iPSC lines and derivative stocks Plan describing the generation of the initial stock and appropriate strategies and workflows for the rebanking of highindemand iPSC lines and iPSCderived cells as well as externally submitted assets Protocols for cryopreservation of bulk quantities of iPSC and iPSC-derived cells established Cryopreservation protocols for bulk quantities of iPSC and iPSCderived cells Assessment of drug efficacy conducted Report on the assessment of drug efficacy and maturation Data management handbook Report incl SOPs describing the concepts for data security backup protection and access regulations to provide safe secure and uptodate technical hardware structures Protocol and workflow for optimized hepatocyte differentiation established PoC models completed and data transferred to WP5 gene editing tools established and EFPIA motivated cell line generation completed and transferred to WP3 Enhanced protocols for large-scale iPSC differentiation established Set of refined large-scale iPSC differentiation protocols QC criteria for progenitor cell types identified QC panel identified and defined for progenitor cells. Panel identification transferred to WP3. Other (5) Automated data validation established Automated IT workflow replaces the initial manual system while ensuring low maintenance, low risk and high automation First induced differentiated GE line generated, characterised and banked for accelerated differentiation and maturation of CNS cell types GE lines established, PoC models completed, data transferred to WP5 and EFPIA motivated cell line generation completed and transferred to WP3 Standard API established for data integration Definition and implementation of data standards and codification for automated data exchange and mapping Fully operable EBiSC2 bank established All required operations have been performed to allow operations of the EBiSC2 bank after Month 3. At least three EFPIA-driven cell line commissioning projects completed Outcome of at least three EFPIA-driven cell line commissioning projects carried out. Websites, patent fillings, videos etc. (2) Establishment of new branding and trademark registration Presentation of the EBiSC2 branding and registration as trademark to ensure the legal framework Portal and interface implemented Portal and website redesigned and launched Pubblicazioni Peer reviewed articles (12) SOX9-induced Generation of Functional Astrocytes Supporting Neuronal Maturation in an All-human System Autori: Katrien Neyrinck, Johanna Van Den Daele, Tim Vervliet, Jonathan De Smedt, Keimpe Wierda, Melissa Nijs, Tom Vanbokhoven, Astrid D’hondt, Mélanie Planque, Sarah-Maria Fendt, Pei-Yu Shih, Frederik Seibt, Juan Pita Almenar, Mohamed Kreir, Devesh Kumar, Vania Broccoli, Geert Bultynck, Andreas Ebneth, Alfredo Cabrera-Socorro, Catherine Verfaillie Pubblicato in: Stem Cell Reviews and Reports, 2021, ISSN 2629-3269 Editore: Springer DOI: 10.1007/s12015-021-10179-x The EBiSC iPSC bank for disease studies Autori: Rachel Steeg, Julia C. Neubauer, Sabine C. Müller, Andreas Ebneth, Heiko Zimmermann Pubblicato in: Stem Cell Research, Issue 49, 2020, Page(s) 102034, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2020.102034 EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines Autori: Rachel Steeg, Sabine C. Mueller, Nancy Mah, Bjørn Holst, Alfredo Cabrera-Socorro, Glyn N. Stacey, Paul A. De Sousa, Aidan Courtney, Heiko Zimmermann Pubblicato in: Stem Cell Reports, Issue 16/8, 2021, Page(s) 1853-1867, ISSN 2213-6711 Editore: Cell Press DOI: 10.1016/j.stemcr.2021.07.009 Corrigendum to “Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line” [Stem Cell Res. 34/1873–5061 (2019) 101349–55] Autori: Benjamin Schmid, Kennie R. Prehn, Natakarn Nimsanor, Blanca Irene Aldana Garcia, Ulla Poulsen, Ida Jørring, Mikkel A. Rasmussen, Christian Clausen, Ulrike A. Mau-Holzmann, Sarayu Ramakrishna, Ravi Muddashetty, Rachel Steeg, Kevin Bruce, Peter Mackintosh, Andreas Ebneth, Bjørn Holst, Alfredo Cabrera-Socorro Pubblicato in: Stem Cell Research, Issue 48, 2020, Page(s) 102005, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2020.102005 Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line Autori: Benjamin Schmid, Kennie R. Prehn, Natakarn Nimsanor, Blanca Irene Aldana Garcia, Ulla Poulsen, Ida Jørring, Mikkel A. Rasmussen, Christian Clausen, Ulrike A. Mau-Holzmann, Sarayu Ramakrishna, Ravi Muddashetty, Rachel Steeg, Kevin Bruce, Peter Mackintosh, Andreas Ebneth, Bjørn Holst, Alfredo Cabrera-Socorro Pubblicato in: Stem Cell Research, Issue 34, 2019, Page(s) 101349, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2018.11.010 Human iPSC-derived hepatocytes in 2D and 3D suspension culture for cryopreservation and in vitro toxicity studies Autori: Saskia Altmaier, Ina Meiser, Emilie Lemesre, Benjamin Chanrion, Rachel Steeg, Lidia Elena Leonte, Bjørn Holst, Boye Schnack Nielsen, Christian Clausen, Katharina Schmidt, Anne Marie Vinggaard, Heiko Zimmermann, Julia Christiane Neubauer, Mikkel Aabech Rasmussen Pubblicato in: Reproductive Toxicology, Issue 111, 2022, Page(s) 68-80, ISSN 0890-6238 Editore: Elsevier BV DOI: 10.1016/j.reprotox.2022.05.005 Development and implementation of large-scale quality control for the European bank for induced Pluripotent Stem Cells Autori: Orla O'Shea, Rachel Steeg, Charlotte Chapman, Peter Mackintosh, Glyn N Stacey Pubblicato in: Stem Cell Research, Issue 45, 2020, Page(s) 101773, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2020.101773 Development of a fully human assay combining NGN2-inducible neurons co-cultured with iPSC-derived astrocytes amenable for electrophysiological studies Autori: Pei-Yu Shih, Mohamed Kreir, Devesh Kumar, Frederik Seibt, Francisco Pestana, Benjamin Schmid, Bjørn Holst, Christian Clausen, Rachel Steeg, Benjamin Fischer, Juan Pita-Almenar, Andreas Ebneth, Alfredo Cabrera-Socorro Pubblicato in: Stem Cell Research, Issue 54, 2021, Page(s) 102386, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2021.102386 Scalable expansion of iPSC and their derivatives across multiple lineages Autori: Chee Keong Kwok, Isabelle Sebastien, Krithika Hariharan, Ina Meiser, Jeanette Wihan, Saskia Altmaier, Isabell Karnatz, Dominic Bauer, Benjamin Fischer, Alexander Feile, Alfredo Cabrera-Socorro, Mikkel Rasmussen, Bjørn Holst, Julia C. Neubauer, Christian Clausen, Catherine Verfaillie, Andreas Ebneth, Mattias Hansson, Rachel Steeg, Heiko Zimmermann Pubblicato in: Reproductive Toxicology, Issue 112, 2022, Page(s) 23-35, ISSN 0890-6238 Editore: Elsevier BV DOI: 10.1016/j.reprotox.2022.05.007 Access to stem cell data and registration of pluripotent cell lines: The Human Pluripotent Stem Cell Registry (hPSCreg) Autori: Nancy Mah, Stefanie Seltmann, Begoña Aran, Rachel Steeg, Johannes Dewender, Nils Bultjer, Anna Veiga, Glyn N. Stacey, Andreas Kurtz Pubblicato in: Stem Cell Research, Issue 47, 2020, Page(s) 101887, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2020.101887 Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor Autori: Jeanette Wihan Pubblicato in: Journal of Visualized Experiments, Issue 6, 2023, ISSN 1940-087X Editore: MYJoVE Corporation DOI: 10.3791/65085 Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus Autori: Benjamin Schmid, Bjørn Holst, Ulla Poulsen, Ida Jørring, Christian Clausen, M. Rasmussen, Ulrike A. Mau-Holzmann, Rachel Steeg, Hugh Nuthall, A. Ebneth, A. Cabrera-Socorro Pubblicato in: Stem Cell Research, Issue 52, 2021, Page(s) 102240, ISSN 1873-5061 Editore: Elsevier DOI: 10.1016/j.scr.2021.102240 Diritti di proprietà intellettuale Trademark (6) EBiSC Numero candidatura/pubblicazione: EP A 06494/19 EP A 06494/19 Data: 2019-05-23 EBiSC Numero candidatura/pubblicazione: EP A 06494/19 EP A 06494/19 Data: 2019-05-23 EBiSC Numero candidatura/pubblicazione: 30 2019 106 778.9 30 2019 106 778 Data: 2019-05-23 EBiSC Numero candidatura/pubblicazione: EP A 06494/19 EP A 06494/19 Data: 2019-05-23 EBiSC Numero candidatura/pubblicazione: 30 2019 106 778.9 30 2019 106 778 Data: 2019-05-23 EBiSC Numero candidatura/pubblicazione: 30 2019 106 778.9 30 2019 106 778 Data: 2019-05-23 È in corso la ricerca di dati su OpenAIRE... Si è verificato un errore durante la ricerca dei dati su OpenAIRE Nessun risultato disponibile
