Next generation precision antibody profiling - from science fiction to reality

Informations projet

TopSpec

N° de convention de subvention: 829157

DOI

10.3030/829157

Projet clôturé

Date de signature de la CE 17 Decembre 2018

Date de début 1 Janvier 2019

Date de fin 31 Decembre 2022

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 3 991 500,00

Contribution de l’UE

€ 3 991 500,00

Coordonné par

KAROLINSKA INSTITUTET
Sweden

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (3)

Draft Exploitation plan and Business strategy document

Draft plan of Exploitation plan and Business strategy document delivered

Scientific reports and publications

Scientific reports and publications delivered

Public demonstrations of TopSpec technology

Other (9)

Logo and Website launch and public accessibility

Project logo and website launch and public accessibility. Site will be constantly maintained and updated.

Fully serviced, functioning Omnitraps & IMS

Fully serviced, functioning Omnitraps.

Young scientist TopSpec technology workshop

Completion of Young scientist TopSpec technology workshop

Modified Omnitraps with updated software

Modified and optimized Omnitraps with updated software (month 33).

Modified Orbitrap Q Exactive HF X installed

Modified Orbitrap Q Exactive HF X delivered and installed at KI - protocol

Two tested, optimized pI-Trap-ESI installed and tested

Two tested, optimized CED guns installed and tested at KI and IP – protocols of tests

Two fully equipped Omnitraps & one IMS installed

Two Omnitraps + one IMS fully equipped with driving electronics, gas lines and pulse

Prototype pI-Trap-ESI installed and tested- protocol

One prototype of the pI-Trap-ESI installed and tested at KI – protocol of test

IP protection strategy finalized

Open Research Data Pilot (1)

Data management plan

Publications

Peer reviewed articles (8)

Exploring frontiers of orbitrap performance for long transients

Auteurs: Eduard Denisov, Eugen Damoc, Alexander Makarov
Publié dans: International Journal of Mass Spectrometry, Numéro 466, 2021, Page(s) 116607, ISSN 1387-3806
Éditeur: Elsevier BV
DOI: 10.1016/j.ijms.2021.116607

Transient-Mediated Simulations of FTMS Isotopic Distributions and Mass Spectra to Guide Experiment Design and Data Analysis.

Auteurs: Konstantin O. Nagornov; Anton N. Kozhinov; Natalia Gasilova; Laure Menin; Yury O. Tsybin
Publié dans: J. Am. Soc. Mass Spectrom., Numéro 1, 2020, Page(s) 1927-1942, ISSN 1044-0305
Éditeur: Elsevier BV
DOI: 10.1021/jasms.0c00190

Structural Analysis of Monoclonal Antibodies with Top-down and Middle-down Electron Transfer Dissociation Mass Spectrometry: The First Decade

Auteurs: Fornelli, L., Ayoub, D., Srzentic, K. Nagornov, K. O. Kozhinov, A. N. Gasilova, N. Menin, L. Beck, A. Tsybin, Y. O.
Publié dans: Chimia, Numéro 2, 2022, Page(s) 114-126, ISSN 0009-4293
Éditeur: Schweizerische Chemische Gedellschaft
DOI: 10.2533/chimia.2022.114

The Omnitrap Platform: A Versatile Segmented Linear Ion Trap for Multidimensional Multiple-Stage Tandem Mass Spectrometry

Auteurs: Dimitris Papanastasiou, Diamantis Kounadis, Alexandros Lekkas, Ioannis Orfanopoulos, Andreas Mpozatzidis, Athanasios Smyrnakis, Elias Panagiotopoulos, Mariangela Kosmopoulou, Maria Reinhardt-Szyba, Kyle Fort, Alexander Makarov, and Roman A. Zubarev
Publié dans: J. Am. Soc. Mass Spectrom., Numéro 3, 2021, Page(s) 1990–2007, ISSN 1044-0305
Éditeur: Elsevier BV
DOI: 10.1021/jasms.2c00214

Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody-Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry.

Auteurs: Konstantin O. Nagornov; Natalia Gasilova; Anton N. Kozhinov; Pasi Virta; Patrik Holm; Laure Menin; Victor J Nesatyy; Yury O. Tsybin
Publié dans: Anal Chem, Numéro 14/9, 2021, Page(s) 12930–12937, ISSN 1520-6882
Éditeur: ACS Publications
DOI: 10.1021/acs.analchem.1c02247

The Human Proteoform Project: Defining the human proteome

Auteurs: Smith, L. M., Agar, J. N., Chamot-Rooke, J., Danis, P. O., Ge, Y., Loo, J. A., Pasa-Tolic, L., Tsybin, Y.O.. Kelleher, N. L. Consortium for Top-Down, Proteomics
Publié dans: Sci Adv, Numéro 12/11, 2021, Page(s) eabk0734, ISSN 2375-2548
Éditeur: Sciense.org
DOI: 10.1126/sciadv.abk0734

Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry.

Auteurs: Kristina Srzentić; Luca Fornelli; Yury O. Tsybin; Joseph A. Loo; Henrique S. Seckler; Jeffrey N. Agar; Lissa C. Anderson; Dina L. Bai; Alain Beck; Jennifer S. Brodbelt; Yuri E. M. van der Burgt; Julia Chamot-Rooke; Sneha Chatterjee; Yunqiu Chen; David Clarke; Paul O. Danis; Jolene K. Diedrich; Robert Anthony D’Ippolito; Mathieu Dupré; Natalia Gasilova; Ying Ge; Young Ah Goo; David R. Goodlett;
Publié dans: J Am Soc Mass Spectrom, Numéro 1, 2020, Page(s) 1783-1802, ISSN 1044-0305
Éditeur: Elsevier BV
DOI: 10.1021/jasms.0c00036

TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics

Auteurs: Dhenin, J., Lima, D. B., Dupre, M., Chamot-Rooke, J.
Publié dans: Bioinformatics, Numéro 18/11, 2021, Page(s) 1136-8, ISSN 1367-4811
Éditeur: Oxford Academic
DOI: 10.1093/bioinformatics/btab784

Other (4)

Adding colour to mass spectra: Charge Determination Analysis (CHARDA) assigns charge state to every ion peak

Auteurs: Y. Lyutvinskiy, K.O. Nagornov, A.N. Kozhinov, N. Gasilova, L. Menin, Z. Meng, X. Zhang, A. Ata Saei, Y.O. Tsybin, A. Makarov, R.A. Zubarev
Publié dans: ChemRxiv, Numéro 17/9, 2021
Éditeur: Cambridge University Press
DOI: 10.26434/chemrxiv-2021-f6n9j

A Novel Family of Quadrupole-Orbitrap Mass Spectrometers for a Broad Range of Analytical Applications

Auteurs: Jan-Peter Hauschild, Amelia Peterson, Erik Couzijn, Eduard Denisov, Denis Chernyshev, Christian Hock, Hamish Stewart, Ralf Hartmer, Dmitry Grinfeld, Christian Thoeing, Bastian Reitemeier, Arne Kreutzmann, Oliver Lange, Wilko Balschun, Aivaras Venckus, Sebastian Kanngiesser, Alexander Kholomeev, Gregor Quiring, Frank Czemper, Eloy Wouters, Michael Belford, Mathias Mueller, Jens Grote, Tabiwang N
Publié dans: Preprints, Numéro 7, 2020
Éditeur: Preprints.org
DOI: 10.20944/preprints202006.0111.v1

Next-generation precision antibody profiling; from science fiction to reality

Auteurs: Roman Zubarev, Dimitris Papanastasiou, Alexander Makarov, Yury Tsybin, Thorleif Lavold,David Kilgour, Julia Chamot-Rooke, Jan Commandeur
Publié dans: The Project Repository Journal, Numéro Volume 15, 2022, Page(s) 38-41
Éditeur: EDMA
DOI: 10.54050/prj1519532

The TopSpec system: new levels in antibody characterisation by top-down mass spectrometry

Auteurs: Roman A. Zubarev, Dimitris Papanastasiou, Julia Chamot-Rooke, Yury O. Tsybin, Alexander Makarov, David P A Kilgour, Thorleif Lavold, Jan Commandeur
Publié dans: The Project Repository Journal, Numéro Volume 16, 2023, Page(s) 42-46
Éditeur: EDMA
DOI: 10.54050/prj1619878

Droits de propriété intellectuelle

Patent (4)

ANALYSIS OF FRAGMENT IONS BY ION MOBILITY SEPARATION FOLLOWED BY MASS ANALYSIS

Numéro de demande/publication: 19 174615
Date: 2019-05-15
Demandeur(s): FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA

METHOD FOR CAPILLARY ISOELECTRIC FOCUSING SEPARATION OF POLYPEPTIDES

Numéro de demande/publication: 20 21064686
Date: 2021-06-01
Demandeur(s): BIOMOTIF AB

SEGMENTED LINEAR ION TRAP FOR ENHANCED ION ACTIVATION AND STORAGE

Numéro de demande/publication: 17 704530
Date: 2017-02-02
Demandeur(s): FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA

APPARATUS AND METHOD FOR ION ANYALYSIS USING MASS SPECTROMETRY

Numéro de demande/publication: 21 170210
Date: 2021-04-23
Demandeur(s): FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA