Next generation precision antibody profiling - from science fiction to reality

Informazioni relative al progetto

TopSpec

ID dell’accordo di sovvenzione: 829157

DOI

10.3030/829157

Progetto chiuso

Data della firma CE 17 Dicembre 2018

Data di avvio 1 Gennaio 2019

Data di completamento 31 Dicembre 2022

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 991 500,00

Contributo UE

€ 3 991 500,00

Coordinato da

KAROLINSKA INSTITUTET
Sweden

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (3)

Draft Exploitation plan and Business strategy document

Draft plan of Exploitation plan and Business strategy document delivered

Scientific reports and publications

Scientific reports and publications delivered

Public demonstrations of TopSpec technology

Other (9)

Logo and Website launch and public accessibility

Project logo and website launch and public accessibility. Site will be constantly maintained and updated.

Fully serviced, functioning Omnitraps & IMS

Fully serviced, functioning Omnitraps.

Young scientist TopSpec technology workshop

Completion of Young scientist TopSpec technology workshop

Modified Omnitraps with updated software

Modified and optimized Omnitraps with updated software (month 33).

Modified Orbitrap Q Exactive HF X installed

Modified Orbitrap Q Exactive HF X delivered and installed at KI - protocol

Two tested, optimized pI-Trap-ESI installed and tested

Two tested, optimized CED guns installed and tested at KI and IP – protocols of tests

Two fully equipped Omnitraps & one IMS installed

Two Omnitraps + one IMS fully equipped with driving electronics, gas lines and pulse

Prototype pI-Trap-ESI installed and tested- protocol

One prototype of the pI-Trap-ESI installed and tested at KI – protocol of test

IP protection strategy finalized

Open Research Data Pilot (1)

Data management plan

Pubblicazioni

Peer reviewed articles (8)

Exploring frontiers of orbitrap performance for long transients

Autori: Eduard Denisov, Eugen Damoc, Alexander Makarov
Pubblicato in: International Journal of Mass Spectrometry, Numero 466, 2021, Pagina/e 116607, ISSN 1387-3806
Editore: Elsevier BV
DOI: 10.1016/j.ijms.2021.116607

Transient-Mediated Simulations of FTMS Isotopic Distributions and Mass Spectra to Guide Experiment Design and Data Analysis.

Autori: Konstantin O. Nagornov; Anton N. Kozhinov; Natalia Gasilova; Laure Menin; Yury O. Tsybin
Pubblicato in: J. Am. Soc. Mass Spectrom., Numero 1, 2020, Pagina/e 1927-1942, ISSN 1044-0305
Editore: Elsevier BV
DOI: 10.1021/jasms.0c00190

Structural Analysis of Monoclonal Antibodies with Top-down and Middle-down Electron Transfer Dissociation Mass Spectrometry: The First Decade

Autori: Fornelli, L., Ayoub, D., Srzentic, K. Nagornov, K. O. Kozhinov, A. N. Gasilova, N. Menin, L. Beck, A. Tsybin, Y. O.
Pubblicato in: Chimia, Numero 2, 2022, Pagina/e 114-126, ISSN 0009-4293
Editore: Schweizerische Chemische Gedellschaft
DOI: 10.2533/chimia.2022.114

The Omnitrap Platform: A Versatile Segmented Linear Ion Trap for Multidimensional Multiple-Stage Tandem Mass Spectrometry

Autori: Dimitris Papanastasiou, Diamantis Kounadis, Alexandros Lekkas, Ioannis Orfanopoulos, Andreas Mpozatzidis, Athanasios Smyrnakis, Elias Panagiotopoulos, Mariangela Kosmopoulou, Maria Reinhardt-Szyba, Kyle Fort, Alexander Makarov, and Roman A. Zubarev
Pubblicato in: J. Am. Soc. Mass Spectrom., Numero 3, 2021, Pagina/e 1990–2007, ISSN 1044-0305
Editore: Elsevier BV
DOI: 10.1021/jasms.2c00214

Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody-Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry.

Autori: Konstantin O. Nagornov; Natalia Gasilova; Anton N. Kozhinov; Pasi Virta; Patrik Holm; Laure Menin; Victor J Nesatyy; Yury O. Tsybin
Pubblicato in: Anal Chem, Numero 14/9, 2021, Pagina/e 12930–12937, ISSN 1520-6882
Editore: ACS Publications
DOI: 10.1021/acs.analchem.1c02247

The Human Proteoform Project: Defining the human proteome

Autori: Smith, L. M., Agar, J. N., Chamot-Rooke, J., Danis, P. O., Ge, Y., Loo, J. A., Pasa-Tolic, L., Tsybin, Y.O.. Kelleher, N. L. Consortium for Top-Down, Proteomics
Pubblicato in: Sci Adv, Numero 12/11, 2021, Pagina/e eabk0734, ISSN 2375-2548
Editore: Sciense.org
DOI: 10.1126/sciadv.abk0734

Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry.

Autori: Kristina Srzentić; Luca Fornelli; Yury O. Tsybin; Joseph A. Loo; Henrique S. Seckler; Jeffrey N. Agar; Lissa C. Anderson; Dina L. Bai; Alain Beck; Jennifer S. Brodbelt; Yuri E. M. van der Burgt; Julia Chamot-Rooke; Sneha Chatterjee; Yunqiu Chen; David Clarke; Paul O. Danis; Jolene K. Diedrich; Robert Anthony D’Ippolito; Mathieu Dupré; Natalia Gasilova; Ying Ge; Young Ah Goo; David R. Goodlett;
Pubblicato in: J Am Soc Mass Spectrom, Numero 1, 2020, Pagina/e 1783-1802, ISSN 1044-0305
Editore: Elsevier BV
DOI: 10.1021/jasms.0c00036

TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics

Autori: Dhenin, J., Lima, D. B., Dupre, M., Chamot-Rooke, J.
Pubblicato in: Bioinformatics, Numero 18/11, 2021, Pagina/e 1136-8, ISSN 1367-4811
Editore: Oxford Academic
DOI: 10.1093/bioinformatics/btab784

Other (4)

Adding colour to mass spectra: Charge Determination Analysis (CHARDA) assigns charge state to every ion peak

Autori: Y. Lyutvinskiy, K.O. Nagornov, A.N. Kozhinov, N. Gasilova, L. Menin, Z. Meng, X. Zhang, A. Ata Saei, Y.O. Tsybin, A. Makarov, R.A. Zubarev
Pubblicato in: ChemRxiv, Numero 17/9, 2021
Editore: Cambridge University Press
DOI: 10.26434/chemrxiv-2021-f6n9j

A Novel Family of Quadrupole-Orbitrap Mass Spectrometers for a Broad Range of Analytical Applications

Autori: Jan-Peter Hauschild, Amelia Peterson, Erik Couzijn, Eduard Denisov, Denis Chernyshev, Christian Hock, Hamish Stewart, Ralf Hartmer, Dmitry Grinfeld, Christian Thoeing, Bastian Reitemeier, Arne Kreutzmann, Oliver Lange, Wilko Balschun, Aivaras Venckus, Sebastian Kanngiesser, Alexander Kholomeev, Gregor Quiring, Frank Czemper, Eloy Wouters, Michael Belford, Mathias Mueller, Jens Grote, Tabiwang N
Pubblicato in: Preprints, Numero 7, 2020
Editore: Preprints.org
DOI: 10.20944/preprints202006.0111.v1

Next-generation precision antibody profiling; from science fiction to reality

Autori: Roman Zubarev, Dimitris Papanastasiou, Alexander Makarov, Yury Tsybin, Thorleif Lavold,David Kilgour, Julia Chamot-Rooke, Jan Commandeur
Pubblicato in: The Project Repository Journal, Numero Volume 15, 2022, Pagina/e 38-41
Editore: EDMA
DOI: 10.54050/prj1519532

The TopSpec system: new levels in antibody characterisation by top-down mass spectrometry

Autori: Roman A. Zubarev, Dimitris Papanastasiou, Julia Chamot-Rooke, Yury O. Tsybin, Alexander Makarov, David P A Kilgour, Thorleif Lavold, Jan Commandeur
Pubblicato in: The Project Repository Journal, Numero Volume 16, 2023, Pagina/e 42-46
Editore: EDMA
DOI: 10.54050/prj1619878

Diritti di proprietà intellettuale

Patent (4)

ANALYSIS OF FRAGMENT IONS BY ION MOBILITY SEPARATION FOLLOWED BY MASS ANALYSIS

Numero candidatura/pubblicazione: 19 174615
Data: 2019-05-15
Candidato/i: FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA

METHOD FOR CAPILLARY ISOELECTRIC FOCUSING SEPARATION OF POLYPEPTIDES

Numero candidatura/pubblicazione: 20 21064686
Data: 2021-06-01
Candidato/i: BIOMOTIF AB

SEGMENTED LINEAR ION TRAP FOR ENHANCED ION ACTIVATION AND STORAGE

Numero candidatura/pubblicazione: 17 704530
Data: 2017-02-02
Candidato/i: FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA

APPARATUS AND METHOD FOR ION ANYALYSIS USING MASS SPECTROMETRY

Numero candidatura/pubblicazione: 21 170210
Data: 2021-04-23
Candidato/i: FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA