Delivery and On-Demand Activation of Chemically Synthesized and Uniquely Modified Proteins in Living Cells

Información del proyecto

SynProAtCell

Identificador del acuerdo de subvención: 831783

DOI

10.3030/831783

Proyecto cerrado

Fecha de la firma de la CE 3 Mayo 2019

Fecha de inicio 1 Octubre 2019

Fecha de finalización 30 Septiembre 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 2 500 000,00

Aportación de la UE

€ 2 500 000,00

Coordinado por

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (21)

The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag

Autores: Indrajit Sahu, Sachitanand M. Mali, Prasad Sulkshane, Cong Xu, Andrey Rozenberg, Roni Morag, Manisha Priyadarsini Sahoo, Sumeet K. Singh, Zhanyu Ding, Yifan Wang, Sharleen Day, Yao Cong, Oded Kleifeld*, Ashraf Brik*, Michael H. Glickman*
Publicado en: Nature Communications, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-26427-0

On-Demand Detachment of Succinimides on Cysteine to Facilitate (Semi)Synthesis of Challenging Proteins

Autores: Ganga B. Vamisetti, Gandhesiri Satish, Prasad Sulkshane, Guy Mann, Michael H. Glickman, Ashraf Brik
Publicado en: Journal of the American Chemical Society, Edición 142/46, 2020, Página(s) 19558-19569, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.0c07663

Enhanced Live-Cell Delivery of Synthetic Proteins Assisted by Cell-Penetrating Peptides Fused to DABCYL

Autores: Shaswati Manda, Guy Mann, Gandhesiri Satish and Ashraf Brik
Publicado en: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202016208

Synthesis of ubiquitinated proteins for biochemical and functional analysis

Autores: Julia Kriegesmann, Ashraf Brik
Publicado en: Chemical Science, Edición 14, 2024, Página(s) 10025-10040, ISSN 2041-6520
Editor: Royal Society of Chemistry
DOI: 10.1039/d3sc03664b

The Development of a Fluorescence‐Based Competitive Assay Enabled the Discovery of Dimeric Cyclic Peptide Modulators of Ubiquitin Chains

Autores: Ganga B. Vamisetti, Roman Meledin, Mickal Nawatha, Hiroaki Suga, Ashraf Brik
Publicado en: Angewandte Chemie International Edition, Edición 60/13, 2021, Página(s) 7018-7023, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202013392

Probing the cell delivery of synthetic diubiquitinchains†

Autores: Shaswati Mandal and Ashraf Brik
Publicado en: Chemical Communications, 2022, Página(s) 58, 8782-8785, ISSN 0009-241X
Editor: Chemical Communications
DOI: 10.1039/d2cc02476d

Synthesis and Delivery of a Stable Phosphorylated Ubiquitin Probe to Study Ubiquitin Conjugation in Mitophagy

Autores: Guy Mann, Gandhesiri Satish, Prasad Sulkshane, Shaswati Mandal , Michael H. Glickman  and Ashraf Brik 
Publicado en: Chemical Communications, 2021, ISSN 1359-7345
Editor: Royal Society of Chemistry
DOI: 10.1039/d1cc04045f

General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Autores: Shay Laps, Fatima Atamleh, Guy Kamnesky, Hao Sun, Ashraf Brik
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-21209-0

Gold(I)-Mediated Rapid Cyclization of Propargylated Peptides via Imine Formation

Autores: Rajeshwer Vanjari, Deepanjan Panda, Shaswati Mandal, Ganga B. Vamisetti, and Ashraf Brik*
Publicado en: Journal of the American Chemical Society, 2022, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.1c12906

Synthetic Proteins behind the Plasma Barrier: Molecular Spies

Autores: Guy Mann, Pradeep Sadhu, and Ashraf Brik*
Publicado en: Accounts of Chemical Research, Edición 00014842, 2022, Página(s) 5, 15, 2055–2067, ISSN 0001-4842
Editor: American Chemical Society
DOI: 10.1021/acs.accounts.2c00236

Examining the role of phosphorylation of p19 INK4d in its stability and ubiquitination using chemical protein synthesis

Autores: Muna Msallam, Hao Sun, Roman Meledin, Pauline Franz, Ashraf Brik
Publicado en: Chemical Science, Edición 11/21, 2020, Página(s) 5526-5531, ISSN 2041-6520
Editor: Royal Society of Chemistry
DOI: 10.1039/c9sc06300e

Chemical approaches to explore ubiquitin-like proteins

Autores: Reem Mousa, Dana Shkolnik, Yam Alalouf, Ashraf Brik
Publicado en: RSC Chemical Biology, Edición 6, 2025, Página(s) 492-509, ISSN 2633-0679
Editor: Royal Society of Chemistry (RSC)
DOI: 10.1039/d4cb00220b

Antibody for Serine 65 Phosphorylated Ubiquitin Identifies PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1

Autores: Guy Mann, Prasad Sulkshane, Pradeep Sadhu ,Tamar Ziv, Michael H. Glickman* and Ashraf Brik*
Publicado en: Molecules, Edición 14203049, 2022, ISSN 1420-3049
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27154867

Suspension Bead Loading (SBL): An Economical Protein Delivery Platform to Study URM1’s Behavior in Live Cells

Autores: Abhishek Saha, Reem Mousa, Yam Alalouf, Pradeep Sadhu, Mahdi Hasan, Shaswati Mandal, Guy Mann, Ashraf Brik
Publicado en: Angewandte Chemie International Edition, Edición 63, 2025, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202410135

Multiplexed Delivery of Synthetic (Un)Conjugatable Ubiquitin and SUMO2 Enables Simultaneous Monitoring of their Localization and Function in Live Cells

Autores: Guy Mann, Pradeep Sadhu, Ashraf Brik
Publicado en: ChemBioChem, 2022, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200122

Combining Chemical Protein Synthesis and Random Nonstandard Peptides Integrated Discovery for Modulating Biological Processes

Autores: Abhishek Saha, Hiroaki Suga, Ashraf Brik
Publicado en: Accounts of Chemical Research, Edición 56, 2023, Página(s) 1953-1965, ISSN 0001-4842
Editor: American Chemical Society
DOI: 10.1021/acs.accounts.3c00178

Mechanism of selective recognition of Lys48-linked polyubiquitin by macrocyclic peptide inhibitors of proteasomal degradation

Autores: Betsegaw Lemma, Di Zhang, Ganga B. Vamisetti, Bryan G. Wentz, Hiroaki Suga, Ashraf Brik, Jacek Lubkowski, David Fushman
Publicado en: Nature Communications, Edición 14, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-43025-4

A Highly Efficient Cyclization Approach of Propargylated Peptides via Gold(I)-Mediated Sequential C-N, C-O and C-C Bond Formation

Autores: Rajeshwer Vanjari, Emad Eid, Ganga B. Vamisetti, Shaswati Mandal, Ashraf Brik*
Publicado en: ACS Central Science, 2021, ISSN 2374-7943
Editor: ACS Central Science
DOI: 10.1021/acscentsci.1c00969

In vivo modulation of ubiquitin chains by N -methylated non-proteinogenic cyclic peptides

Autores: Joseph M. Rogers, Mickal Nawatha, Betsegaw Lemma, Ganga B. Vamisetti, Ido Livneh, Uri Barash, Israel Vlodavsky, Aaron Ciechanover, David Fushman, Hiroaki Suga, Ashraf Brik
Publicado en: RSC Chemical Biology, Edición 2/2, 2021, Página(s) 513-522, ISSN 2633-0679
Editor: RSC
DOI: 10.1039/d0cb00179a

Harnessing the power of transition metals in solid-phase peptide synthesis and key steps in the (semi)synthesis of proteins

Autores: Shay Laps, Gandhesiri Satish, Ashraf Brik
Publicado en: Chemical Society Reviews, Edición 50/4, 2021, Página(s) 2367-2387, ISSN 0306-0012
Editor: Royal Society of Chemistry
DOI: 10.1039/d0cs01156h

Total Chemical Synthesis of ISGylated-Ubiquitin Hybrid Chain Assisted by Acetamidomethyl Derivatives with Dual Functions

Autores: Emad Eid, Gábor N. Boross, Hao Sun, Muna Msallam, Sumeet K. Singh, Ashraf Brik
Publicado en: Bioconjugate Chemistry, Edición 31/3, 2020, Página(s) 889-894, ISSN 1043-1802
Editor: American Chemical Society
DOI: 10.1021/acs.bioconjchem.0c00026