Delivery and On-Demand Activation of Chemically Synthesized and Uniquely Modified Proteins in Living Cells

Informazioni relative al progetto

SynProAtCell

ID dell’accordo di sovvenzione: 831783

DOI

10.3030/831783

Progetto chiuso

Data della firma CE 3 Maggio 2019

Data di avvio 1 Ottobre 2019

Data di completamento 30 Settembre 2025

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 2 500 000,00

Contributo UE

€ 2 500 000,00

Coordinato da

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (16)

The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag

Autori: Indrajit Sahu, Sachitanand M. Mali, Prasad Sulkshane, Cong Xu, Andrey Rozenberg, Roni Morag, Manisha Priyadarsini Sahoo, Sumeet K. Singh, Zhanyu Ding, Yifan Wang, Sharleen Day, Yao Cong, Oded Kleifeld*, Ashraf Brik*, Michael H. Glickman*
Pubblicato in: Nature Communications, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-26427-0

On-Demand Detachment of Succinimides on Cysteine to Facilitate (Semi)Synthesis of Challenging Proteins

Autori: Ganga B. Vamisetti, Gandhesiri Satish, Prasad Sulkshane, Guy Mann, Michael H. Glickman, Ashraf Brik
Pubblicato in: Journal of the American Chemical Society, Numero 142/46, 2020, Pagina/e 19558-19569, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.0c07663

Enhanced Live-Cell Delivery of Synthetic Proteins Assisted by Cell-Penetrating Peptides Fused to DABCYL

Autori: Shaswati Manda, Guy Mann, Gandhesiri Satish and Ashraf Brik
Pubblicato in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202016208

The Development of a Fluorescence‐Based Competitive Assay Enabled the Discovery of Dimeric Cyclic Peptide Modulators of Ubiquitin Chains

Autori: Ganga B. Vamisetti, Roman Meledin, Mickal Nawatha, Hiroaki Suga, Ashraf Brik
Pubblicato in: Angewandte Chemie International Edition, Numero 60/13, 2021, Pagina/e 7018-7023, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202013392

Probing the cell delivery of synthetic diubiquitinchains†

Autori: Shaswati Mandal and Ashraf Brik
Pubblicato in: Chemical Communications, Numero 0009241X, 2022, Pagina/e 58, 8782-8785, ISSN 0009-241X
Editore: Chemical Communications
DOI: 10.1039/d2cc02476d

Synthesis and Delivery of a Stable Phosphorylated Ubiquitin Probe to Study Ubiquitin Conjugation in Mitophagy

Autori: Guy Mann, Gandhesiri Satish, Prasad Sulkshane, Shaswati Mandal , Michael H. Glickman  and Ashraf Brik 
Pubblicato in: Chemical Communications, 2021, ISSN 1359-7345
Editore: Royal Society of Chemistry
DOI: 10.1039/d1cc04045f

General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Autori: Shay Laps, Fatima Atamleh, Guy Kamnesky, Hao Sun, Ashraf Brik
Pubblicato in: Nature Communications, Numero 12/1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-21209-0

Gold(I)-Mediated Rapid Cyclization of Propargylated Peptides via Imine Formation

Autori: Rajeshwer Vanjari, Deepanjan Panda, Shaswati Mandal, Ganga B. Vamisetti, and Ashraf Brik*
Pubblicato in: Journal of the American Chemical Society, 2022, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.1c12906

Synthetic Proteins behind the Plasma Barrier: Molecular Spies

Autori: Guy Mann, Pradeep Sadhu, and Ashraf Brik*
Pubblicato in: Accounts of Chemical Research, Numero 00014842, 2022, Pagina/e 5, 15, 2055–2067, ISSN 0001-4842
Editore: American Chemical Society
DOI: 10.1021/acs.accounts.2c00236

Examining the role of phosphorylation of p19 INK4d in its stability and ubiquitination using chemical protein synthesis

Autori: Muna Msallam, Hao Sun, Roman Meledin, Pauline Franz, Ashraf Brik
Pubblicato in: Chemical Science, Numero 11/21, 2020, Pagina/e 5526-5531, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/c9sc06300e

Antibody for Serine 65 Phosphorylated Ubiquitin Identifies PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1

Autori: Guy Mann, Prasad Sulkshane, Pradeep Sadhu ,Tamar Ziv, Michael H. Glickman* and Ashraf Brik*
Pubblicato in: Molecules, Numero 14203049, 2022, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27154867

Multiplexed Delivery of Synthetic (Un)Conjugatable Ubiquitin and SUMO2 Enables Simultaneous Monitoring of their Localization and Function in Live Cells

Autori: Guy Mann, Pradeep Sadhu, Ashraf Brik
Pubblicato in: ChemBioChem, 2022, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200122

A Highly Efficient Cyclization Approach of Propargylated Peptides via Gold(I)-Mediated Sequential C-N, C-O and C-C Bond Formation

Autori: Rajeshwer Vanjari, Emad Eid, Ganga B. Vamisetti, Shaswati Mandal, Ashraf Brik*
Pubblicato in: ACS Central Science, 2021, ISSN 2374-7943
Editore: ACS Central Science
DOI: 10.1021/acscentsci.1c00969

In vivo modulation of ubiquitin chains by N -methylated non-proteinogenic cyclic peptides

Autori: Joseph M. Rogers, Mickal Nawatha, Betsegaw Lemma, Ganga B. Vamisetti, Ido Livneh, Uri Barash, Israel Vlodavsky, Aaron Ciechanover, David Fushman, Hiroaki Suga, Ashraf Brik
Pubblicato in: RSC Chemical Biology, Numero 2/2, 2021, Pagina/e 513-522, ISSN 2633-0679
Editore: RSC
DOI: 10.1039/d0cb00179a

Harnessing the power of transition metals in solid-phase peptide synthesis and key steps in the (semi)synthesis of proteins

Autori: Shay Laps, Gandhesiri Satish, Ashraf Brik
Pubblicato in: Chemical Society Reviews, Numero 50/4, 2021, Pagina/e 2367-2387, ISSN 0306-0012
Editore: Royal Society of Chemistry
DOI: 10.1039/d0cs01156h

Total Chemical Synthesis of ISGylated-Ubiquitin Hybrid Chain Assisted by Acetamidomethyl Derivatives with Dual Functions

Autori: Emad Eid, Gábor N. Boross, Hao Sun, Muna Msallam, Sumeet K. Singh, Ashraf Brik
Pubblicato in: Bioconjugate Chemistry, Numero 31/3, 2020, Pagina/e 889-894, ISSN 1043-1802
Editore: American Chemical Society
DOI: 10.1021/acs.bioconjchem.0c00026