Delivery and On-Demand Activation of Chemically Synthesized and Uniquely Modified Proteins in Living Cells

Project Information

SynProAtCell

Grant agreement ID: 831783

DOI

10.3030/831783

Project closed

EC signature date 3 May 2019

Start date 1 October 2019

End date 30 September 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 500 000,00

EU contribution

€ 2 500 000,00

Coordinated by

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (16)

The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag

Author(s): Indrajit Sahu, Sachitanand M. Mali, Prasad Sulkshane, Cong Xu, Andrey Rozenberg, Roni Morag, Manisha Priyadarsini Sahoo, Sumeet K. Singh, Zhanyu Ding, Yifan Wang, Sharleen Day, Yao Cong, Oded Kleifeld*, Ashraf Brik*, Michael H. Glickman*
Published in: Nature Communications, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-26427-0

On-Demand Detachment of Succinimides on Cysteine to Facilitate (Semi)Synthesis of Challenging Proteins

Author(s): Ganga B. Vamisetti, Gandhesiri Satish, Prasad Sulkshane, Guy Mann, Michael H. Glickman, Ashraf Brik
Published in: Journal of the American Chemical Society, Issue 142/46, 2020, Page(s) 19558-19569, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c07663

Enhanced Live-Cell Delivery of Synthetic Proteins Assisted by Cell-Penetrating Peptides Fused to DABCYL

Author(s): Shaswati Manda, Guy Mann, Gandhesiri Satish and Ashraf Brik
Published in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202016208

The Development of a Fluorescence‐Based Competitive Assay Enabled the Discovery of Dimeric Cyclic Peptide Modulators of Ubiquitin Chains

Author(s): Ganga B. Vamisetti, Roman Meledin, Mickal Nawatha, Hiroaki Suga, Ashraf Brik
Published in: Angewandte Chemie International Edition, Issue 60/13, 2021, Page(s) 7018-7023, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202013392

Probing the cell delivery of synthetic diubiquitinchains†

Author(s): Shaswati Mandal and Ashraf Brik
Published in: Chemical Communications, Issue 0009241X, 2022, Page(s) 58, 8782-8785, ISSN 0009-241X
Publisher: Chemical Communications
DOI: 10.1039/d2cc02476d

Synthesis and Delivery of a Stable Phosphorylated Ubiquitin Probe to Study Ubiquitin Conjugation in Mitophagy

Author(s): Guy Mann, Gandhesiri Satish, Prasad Sulkshane, Shaswati Mandal , Michael H. Glickman  and Ashraf Brik 
Published in: Chemical Communications, 2021, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1cc04045f

General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Author(s): Shay Laps, Fatima Atamleh, Guy Kamnesky, Hao Sun, Ashraf Brik
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-21209-0

Gold(I)-Mediated Rapid Cyclization of Propargylated Peptides via Imine Formation

Author(s): Rajeshwer Vanjari, Deepanjan Panda, Shaswati Mandal, Ganga B. Vamisetti, and Ashraf Brik*
Published in: Journal of the American Chemical Society, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.1c12906

Synthetic Proteins behind the Plasma Barrier: Molecular Spies

Author(s): Guy Mann, Pradeep Sadhu, and Ashraf Brik*
Published in: Accounts of Chemical Research, Issue 00014842, 2022, Page(s) 5, 15, 2055–2067, ISSN 0001-4842
Publisher: American Chemical Society
DOI: 10.1021/acs.accounts.2c00236

Examining the role of phosphorylation of p19 INK4d in its stability and ubiquitination using chemical protein synthesis

Author(s): Muna Msallam, Hao Sun, Roman Meledin, Pauline Franz, Ashraf Brik
Published in: Chemical Science, Issue 11/21, 2020, Page(s) 5526-5531, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sc06300e

Antibody for Serine 65 Phosphorylated Ubiquitin Identifies PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1

Author(s): Guy Mann, Prasad Sulkshane, Pradeep Sadhu ,Tamar Ziv, Michael H. Glickman* and Ashraf Brik*
Published in: Molecules, Issue 14203049, 2022, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27154867

Multiplexed Delivery of Synthetic (Un)Conjugatable Ubiquitin and SUMO2 Enables Simultaneous Monitoring of their Localization and Function in Live Cells

Author(s): Guy Mann, Pradeep Sadhu, Ashraf Brik
Published in: ChemBioChem, 2022, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200122

A Highly Efficient Cyclization Approach of Propargylated Peptides via Gold(I)-Mediated Sequential C-N, C-O and C-C Bond Formation

Author(s): Rajeshwer Vanjari, Emad Eid, Ganga B. Vamisetti, Shaswati Mandal, Ashraf Brik*
Published in: ACS Central Science, 2021, ISSN 2374-7943
Publisher: ACS Central Science
DOI: 10.1021/acscentsci.1c00969

In vivo modulation of ubiquitin chains by N -methylated non-proteinogenic cyclic peptides

Author(s): Joseph M. Rogers, Mickal Nawatha, Betsegaw Lemma, Ganga B. Vamisetti, Ido Livneh, Uri Barash, Israel Vlodavsky, Aaron Ciechanover, David Fushman, Hiroaki Suga, Ashraf Brik
Published in: RSC Chemical Biology, Issue 2/2, 2021, Page(s) 513-522, ISSN 2633-0679
Publisher: RSC
DOI: 10.1039/d0cb00179a

Harnessing the power of transition metals in solid-phase peptide synthesis and key steps in the (semi)synthesis of proteins

Author(s): Shay Laps, Gandhesiri Satish, Ashraf Brik
Published in: Chemical Society Reviews, Issue 50/4, 2021, Page(s) 2367-2387, ISSN 0306-0012
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cs01156h

Total Chemical Synthesis of ISGylated-Ubiquitin Hybrid Chain Assisted by Acetamidomethyl Derivatives with Dual Functions

Author(s): Emad Eid, Gábor N. Boross, Hao Sun, Muna Msallam, Sumeet K. Singh, Ashraf Brik
Published in: Bioconjugate Chemistry, Issue 31/3, 2020, Page(s) 889-894, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.0c00026