The project had tree scientific aims: i. to test the in vivo function of the three candidate genes, and in particular, their potential involvement in cell migration; ii. for those actually controlling cell migration, unravel their direct effect on actin dynamics as well as their subcellular localization; iii. for those controlling cell migration, position them in feedback loops. The first two aims were achieved, with in particular, one gene, named nhsl1b, that appeared to control the migration of some important progenitor cells in the early embryo. I could show that is does so regulating the length and lifetime of actin-rich extensions used by cells to migrate. Aim 3 ran into technical and Covid induced difficulties. However, while facing these difficulties, I uncovered an unexpected role of an actin regulator in controlling the formation of cilia (small motile extensions produced by some cells, and essential to the function of many organs, including kydneys or inner-ear). I therefore decided to fully characterize this exciting new function, in a collaboration with a lab at the Pasteur Institute.
These scientific results have been disseminated through my participation to 4 conferences, and through the publication of 4 articles. They were also shared with a wider audience, through a booth at the “fête de la science” (science fair), and by hosting in the lab middle and high school students.
To allow a career restart, the project has enabled me to broaden my areas of expertise. In particular, I received formal and practical training in the use of the zebrafish as a model system, as well as acquired key skills in cell migration analysis. As the host laboratory is highly interdisciplinary, I directly benefited from the interaction with my physicist colleagues, and acquired strong knowledge and know-how in cutting-edge microscopy techniques, including confocal, two-photon and light sheet microscopy. Conversely, I was able to transfer my expertise in developmental biology to some of my colleagues’ the research projects and develop fruitful collaborations.
The project gave me the opportunity to teach 100 hours, gaining crucial experience for my applications to academic positions. I was in charge on the scientific and financial management of the project, and supervised students and a technician, therefore preparing myself for the role of a principal investigator.
