Probing Ultrafast Stereochemical Dynamics by Femtosecond Electronic Circular Dichroism Spectroscopy

Descripción del proyecto

¡Luces, cámara y acción! Observar la dinámica de las interacciones moleculares

La quiralidad es una propiedad interesante de numerosas moléculas biológicas por la cual imágenes especulares (mismos átomos unidos en los mismos lugares) no pueden superponerse. Por ejemplo, nuestras manos: las imágenes especulares muestran que el meñique queda superpuesto sobre el pulgar al colocar una mano encima de la otra. La luz polarizada permite estudiar moléculas quirales con una gran sensibilidad estructural, y se utiliza ampliamente para examinar moléculas biológicas importantes, como las proteínas, los ácidos nucleicos, los glúcidos y los medicamentos. El proyecto CHIRALSCOPY, financiado con fondos europeos, combina esta técnica de examen estructural altamente sensible con la espectroscopia óptica ultrarrápida para permitir la generación de «películas» sobre los cambios estructurales durante reacciones químicas y procesos biológicos.

Objetivo

Since the structure of a (bio)-molecule is crucially linked to its biochemical functions, following its evolution in the course of a chemical reaction or biological process would allow one to gain fundamental insights into the reaction mechanisms and pathways. This calls for optical spectroscopy techniques capable of recording structural dynamics with high time resolution, down to the femtosecond regime. Circular dichroism (CD), i.e. the difference in absorption of left- and right-handed circularly polarised light, is a sensitive probe of the structure of chiral molecules. CHIRALSCOPY aims to develop an innovative time-resolved CD spectrometer which combines the structural sensitivity of steady-state CD spectroscopy with the high time resolution of ultrafast nonlinear optical spectroscopy. CHIRALSCOPY will adopt an innovative approach which directly measures in the time domain the chiral light field from a molecule by combining interferometric detection with optical heterodyne amplification. The state-of-the-art time-resolved CD instrument will be used to elucidate structural dynamics during a prototypical biochemical reaction. CHIRALSCOPY will equip the Experienced Researcher with new knowledge and skills in advanced optical technologies and biophysics, thus broadening his scientific background and enhancing his prospects as an independent researcher. At the same time, the Action and the Host Institution will benefit from the advanced knowledge in ultrafast spectroscopy acquired by Researcher during his PhD thesis. CHIRALSCOPY promises to open new vistas in the field of ultrafast optical spectroscopy enabling one to interrogate molecular dynamics with femtosecond temporal resolution and exquisite structural sensitivity−thus realising the chemist’s dream of a capturing a ‘molecular-motion-picture’ of a chemical reaction.