Descrizione del progetto
Luci, camera, azione: osservare interazioni molecolari dinamiche in movimento
La chiralità è una proprietà interessante di molte molecole biologiche in cui le immagini speculari, ovvero gli stessi atomi attaccati agli stessi posti, non possono essere sovrapposte. Le mani umane ne sono un esempio: immagini speculari in cui il mignolo viene sovrapposto al pollice quando si mette una mano sopra l’altra. La luce polarizzata può sondare molecole chirali con grande sensibilità strutturale, ed è ampiamente utilizzata per studiare molecole biologicamente importanti, quali proteine, acidi nucleici, carboidrati e farmaci. Il progetto CHIRALSCOPY, finanziato dall’UE, sta combinando questa tecnica di esplorazione strutturale altamente sensibile con la spettroscopia ottica ultraveloce per attivare «film» dei cambiamenti strutturali che avvengono durante le reazioni chimiche e i processi biologici.
Obiettivo
Since the structure of a (bio)-molecule is crucially linked to its biochemical functions, following its evolution in the course of a chemical reaction or biological process would allow one to gain fundamental insights into the reaction mechanisms and pathways. This calls for optical spectroscopy techniques capable of recording structural dynamics with high time resolution, down to the femtosecond regime. Circular dichroism (CD), i.e. the difference in absorption of left- and right-handed circularly polarised light, is a sensitive probe of the structure of chiral molecules. CHIRALSCOPY aims to develop an innovative time-resolved CD spectrometer which combines the structural sensitivity of steady-state CD spectroscopy with the high time resolution of ultrafast nonlinear optical spectroscopy. CHIRALSCOPY will adopt an innovative approach which directly measures in the time domain the chiral light field from a molecule by combining interferometric detection with optical heterodyne amplification. The state-of-the-art time-resolved CD instrument will be used to elucidate structural dynamics during a prototypical biochemical reaction. CHIRALSCOPY will equip the Experienced Researcher with new knowledge and skills in advanced optical technologies and biophysics, thus broadening his scientific background and enhancing his prospects as an independent researcher. At the same time, the Action and the Host Institution will benefit from the advanced knowledge in ultrafast spectroscopy acquired by Researcher during his PhD thesis. CHIRALSCOPY promises to open new vistas in the field of ultrafast optical spectroscopy enabling one to interrogate molecular dynamics with femtosecond temporal resolution and exquisite structural sensitivity−thus realising the chemist’s dream of a capturing a ‘molecular-motion-picture’ of a chemical reaction.
Campo scientifico
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF-EF-ST - Standard EFCoordinatore
20133 Milano
Italia