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Identification and characterization of enteric nervous system stem cells

Descripción del proyecto

La caracterización celular y molecular del «segundo cerebro»

El sistema nervioso entérico (SNE) es el sistema nervioso intrínseco del intestino y recubre las paredes del tubo gastrointestinal. Interactúa con la actividad del sistema inmunitario intestinal, la barrera epitelial y la microbiota para controlar la homeostasis intestinal. El objetivo del proyecto IDENSTEM, financiado con fondos europeos, es caracterizar los mecanismos celulares subyacentes al funcionamiento del SNE. Los investigadores se centrarán en una población específica de células gliales entéricas e investigarán su función en un estado estable y en respuesta a lesiones. Además, les interesa examinar la influencia de estas células sobre la inflamación intestinal, un rasgo característico de numerosas enfermedades intestinales.

Objetivo

The digestive system is essential for water and nutrient uptake, waste removal and serves as a sensory system providing information to the central nervous system via the gut-brain communication axis. The gastrointestinal tract harbours the largest collection of neurons and glial cells collectively known as the enteric nervous system (ENS) and regulate digestive physiology independently of brain input. Intestinal function and homeostasis depend on the integrated and balanced activity of multiple gut tissues,in which, the ENS plays a critical role by actively interacting with the intestinal immune system, the epithelial barrier and microbiota. Developmental deficits or acquired disorders in any of these tissue components can result in debilitating gastrointestinal conditions, such as Hirschsprung disease (congenital megacolon) or inflammatory bowel disease. Despite critical contributions of the ENS to digestive physiology and intestinal homeostasis, very little is known about the cellular mechanisms that underpin its function at steady state conditions or in response to injury. IDENSTEM will address the identity and properties of ENS neural stem cells (ENSCs) using the mouse as an experimental model organism. Preliminary data suggest that a subpopulation of enteric glial cells expressing the Notch signalling target Hes5, undergo low rate proliferation and exhibit neurogenic potential. In this proposal, we aim to identify and characterize the cellular and molecular properties of this population. We wish to understand their contribution in maintaining ENS integrity under normal conditions or in response to injury/disease. These studies will advance fundamental neurogastroenterology and the development of novel therapeutic strategies for various gut disorders. Furthermore, they will provide insight into ENS contributions to the gut tissue circuitry and in intestinal inflammation. IDENSTEM will open a new window of knowledge to improve human health.

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural.

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Régimen de financiación

MSCA-IF-EF-ST - Standard EF

Coordinador

THE FRANCIS CRICK INSTITUTE LIMITED
Aportación neta de la UEn
€ 212 933,76
Dirección
1 MIDLAND ROAD
NW1 1AT London
Reino Unido

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Región
London Inner London — West Camden and City of London
Tipo de actividad
Research Organisations
Enlaces
Coste total
€ 212 933,76