Description du projet
Un nouveau test de la thrombose
L’antithrombine (AT) est une protéine secrétée par le foie qui régule le système de coagulation en inactivant certaines enzymes dans la voie de la coagulation du sang. Les tests visant à diagnostiquer la déficience en AT s’appuient sur son activité et ne tiennent pas compte de l’existence de nombreuses protéoformes différentes et de leur signification clinique. Le projet HarmonizATforms, financé par l’UE, entend harmoniser les tests d’AT et concevoir un essai qui génère des résultats pertinents sur le plan clinique. Les chercheurs examinent toutes les AT cliniquement pertinentes et développeront un test basé sur la spectrométrie de masse plutôt que sur un immuno-essai, à savoir la référence absolue actuellement. Le test devrait fournir un meilleur diagnostic dans les domaines de la thrombose et de l’hémostase.
Objectif
Current medical tests for antithrombin deficiency generally measure the overall activity, being blind for the actual proteoforms of AT that contribute to the test. To ensure accurate test results, a traceability chain needs to be in place, which requires the accurate definition of the exact analyte to be measured. I hypothesize that an in-depth understanding of the pathological molecular proteoforms of antithrombin (AT) will allow for the identification of clinically relevant proteoforms and enable test harmonization, resulting in a test with a well-defined clinical outcome that are actionable by clinicians. Mass spectrometry (MS) is an emerging technique in the field of clinical chemistry, which is well suited for the detection of proteoform characteristics and outperforms testing methods based on immunoassays. Therefore my aims are 1. To develop a mass spectrometry based test for the quantification of individual characteristics of molecular proteoforms of AT. 2. Analytically validate the developed method according to clinical chemistry procedures, and 3. Assess the role of MS based test for the standardization of AT tests. I am a passionate scientist with a strong background in the development of MS-based tests for proteins. I plan to perform the project in collaboration with my supervisor, who has large experience in medical test development and the ECAT foundation, who is specialized in proficiency testing of AT and test harmonization. This project will allow me to obtain skills and experience to oversee and develop a clinical test from biomarker discover to test evaluation and implementation, specifically in the niche of thrombosis and haemostasis, as well as the transferable skills required to eventually establish my own independent research group.
Champ scientifique
Programme(s)
Régime de financement
MSCA-IF-EF-RI - RI – Reintegration panelCoordinateur
2333 ZA Leiden
Pays-Bas