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Plasma cell heterogeneity and dynamics in patient tumors

Descripción del proyecto

El papel de los plasmocitos en el cáncer de mama

Las células inmunitarias que se infiltran en el microentorno de los tumores constituyen unas dianas terapéuticas antineoplásicas prometedoras. Sin embargo, los esfuerzos en inmunoterapia se han centrado sobre todo en dirigirse a los linfocitos T, lo que ha tenido diversos resultados. El proyecto financiado con fondos europeos PCinBC se centrará en los plásmidos que se infiltran en los tumores, linfocitos B de producción de anticuerpos que se han asociado con un pronóstico favorable en el cáncer. Los investigadores estudiarán la heterogeneidad y la distribución espacial de los plásmidos en el cáncer de mama e identificarán las moléculas clave responsables de su interacción con el estroma tumoral. Los resultados ayudarán a desvelar el papel de los plásmidos en la inmunidad tumoral, lo que abrirá vías para nuevas inmunoterapias.

Objetivo

During the last decade, the ability to treat cancer by targeting the immune system has revolutionized cancer care, but many patients still fail to respond to current immunotherapies, which mainly target T cells. Besides T cells, the tumor microenvironment is home to many other immune cells; however, we are still unable to accurately predict and target the functions of most tumor-infiltrating immune cells, particularly in human cancer. These cells and their tumor-associated functions represent an untapped reservoir of therapeutic targets. Plasma cells (PC), which are antibody-producing cells derived from B cells, frequently infiltrate solid tumors and their presence associates with positive prognosis across cancer types; yet, our knowledge of tumor-infiltrating PC remains limited. The goal of this project is two-fold: first, I aim to use single cell RNA-seq coupled with spatial transcriptomics (developed by the host lab) to define the heterogeneity and spatial distribution of PC (and their subsets) relative to other cells/histological features in patient breast tumors. PC receptor expression and survival factors will be mapped to generate a PC-tumor stroma 'interactome', which should facilitate defining potential vantage points for therapy. Second, I aim to define the cellular age of tumor-infiltrating PC, since knowing how cells are replaced in a tissue could be highly relevant to both uncover fundamental cell turnover mechanisms and to define new therapeutic avenues. The combination of skillsets between the applicant (tumor immunology) and the host lab (spatial transcriptomics and carbon dating, the latter which uniquely can be used to determine cellular lifespan in humans) presents a unique opportunity for the feasibility and knowledge exchange involved in performing this work. This study’s results could help clarify fundamental questions regarding the biology of tumor-infiltrating plasma cells and help uncover novel anticancer therapeutic targets.

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural.

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Coordinador

KAROLINSKA INSTITUTET
Aportación neta de la UEn
€ 203 852,16
Dirección
Nobels Vag 5
17177 Stockholm
Suecia

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Región
Östra Sverige Stockholm Stockholms län
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 203 852,16