An innovative platform for the design of highly selective ligands
The development of ligands with high specificity against a particular target is of particular interest to biotechnology and therapeutics. However, producing high-affinity ligands that target many epitopes of a single molecule is challenging. The EU-funded APTAFRAME project has developed a new platform for the discovery of antibodies and aptamer nucleotides or peptides that bind to specific molecules. This platform combines DNA origami structures as a molecular 3D framework with structural feedback from cryo-electron microscopy reconstructions to aid the design of cooperative ligands for highly specific target recognition. The APTAFRAME platform is expected to find application in biosensor development, proteome analysis and diagnostics.