Descripción del proyecto DEENESFRITPL Nuevos biosensores para las fábricas de producción microbiana Las fábricas de células microbianas representan una alternativa económica y sostenible para la producción de productos farmacéuticos y de productos químicos industriales. La carencia de métodos de alto rendimiento para la supervisión de la acumulación del producto supone uno de los obstáculos en este proceso. El proyecto GPCR-Sensor, financiado con fondos europeos, pretende utilizar receptores acoplados a la proteína G (GPCR, por sus siglas en inglés), que son los sensores ambientales de las eucariotas, para crear un biosensor «in vivo» versátil. La investigación aplicará la biología sintética y un método interdisciplinar para desarrollar un biosensor GPCR basado en levaduras para el diseño de enzimas y hospedadores de producción. La estructura modular del biosensor permitirá que cualquier GPCR pueda acoplarse, lo que a su vez facilitará una serie de aplicaciones, tanto biomédicas como ambientales. Mostrar el objetivo del proyecto Ocultar el objetivo del proyecto Objetivo Microbial cell factories are an economical and sustainable alternative for the production of pharmaceuticals, industrial chemicals and other valuable chemicals. However, establishing efficient cell factories requires optimized production strains and efficient biosynthetic enzymes, and involves extensive protein and metabolic engineering to achieve industrially relevant yields. One of the main hurdles frequently encountered in this process is the lack of high-throughput methods for monitoring product accumulation. To overcome this limitation, I will harness G-protein coupled receptors (GPCRs), the unique molecular entities that eukaryotes use to sense their environment, to create a versatile in vivo biosensor. I will apply synthetic biology principles and an interdisciplinary approach to develop a yeast-based GPCR biosensor for the engineering of enzymes and production hosts. However, enzyme engineering is only one of the potential applications of GPCR-sensor. Its modular structure permits plugging in any GPCR, enabling a great variety of other powerful applications including bio-medical applications, such as recognition of pathogen epitopes, molecular diagnostics and drug discovery, or environmental applications, such as determining the presence of pollutants from environmental samples. In the GPCR-sensor project, I will combine my knowledge in natural product biosynthesis and enzymology with training in biochemical engineering and biotechnology to acquire a unique expertise that will help me establish my own unique line of research. Furthermore, the comprehensive professional development program, that is an integral part of GPCR-sensor, includes close mentoring in addition to training in fund-rising, research management, communication, teaching and academic leadership. These skills that are Instrumental for launching my future independent internationally competitive research career Ámbito científico engineering and technologyindustrial biotechnologymetabolic engineeringengineering and technologychemical engineeringbiochemical engineeringengineering and technologyenvironmental biotechnologybiosensingnatural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Palabras clave Metabolic engineering Synthetic biology Cell factories High-value compounds Plant specialized metabolites isoprenoids Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2018 - Individual Fellowships Convocatoria de propuestas H2020-MSCA-IF-2018 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinador KOBENHAVNS UNIVERSITET Aportación neta de la UEn € 207 312,00 Dirección NORREGADE 10 1165 Kobenhavn Dinamarca Ver en el mapa Región Danmark Hovedstaden Byen København Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 207 312,00
