Description du projet DEENESFRITPL De nouveaux biocapteurs pour les usines de production microbienne Les usines de cellules microbiennes représentent une alternative économique et durable pour la production de produits pharmaceutiques et de produits chimiques industriels. L’absence de méthodes à haut débit pour contrôler l’accumulation des produits constitue l’un des obstacles à ce processus. Le projet GPCR‑Sensor, financé par l’UE, vise à utiliser les récepteurs couplés aux protéines G (RCPG), les capteurs environnementaux des eucaryotes, afin de créer un biocapteur in vivo polyvalent. La recherche appliquera la biologie synthétique et une approche interdisciplinaire pour développer un biocapteur RCPG basé sur la levure pour l’ingénierie des enzymes et des hôtes de production. La structure modulaire du biocapteur permettra de brancher n’importe quel RCPG, ce qui permettra une variété d’applications, tant biomédicales qu’environnementales. Afficher les objectifs du projet Masquer les objectifs du projet Objectif Microbial cell factories are an economical and sustainable alternative for the production of pharmaceuticals, industrial chemicals and other valuable chemicals. However, establishing efficient cell factories requires optimized production strains and efficient biosynthetic enzymes, and involves extensive protein and metabolic engineering to achieve industrially relevant yields. One of the main hurdles frequently encountered in this process is the lack of high-throughput methods for monitoring product accumulation. To overcome this limitation, I will harness G-protein coupled receptors (GPCRs), the unique molecular entities that eukaryotes use to sense their environment, to create a versatile in vivo biosensor. I will apply synthetic biology principles and an interdisciplinary approach to develop a yeast-based GPCR biosensor for the engineering of enzymes and production hosts. However, enzyme engineering is only one of the potential applications of GPCR-sensor. Its modular structure permits plugging in any GPCR, enabling a great variety of other powerful applications including bio-medical applications, such as recognition of pathogen epitopes, molecular diagnostics and drug discovery, or environmental applications, such as determining the presence of pollutants from environmental samples. In the GPCR-sensor project, I will combine my knowledge in natural product biosynthesis and enzymology with training in biochemical engineering and biotechnology to acquire a unique expertise that will help me establish my own unique line of research. Furthermore, the comprehensive professional development program, that is an integral part of GPCR-sensor, includes close mentoring in addition to training in fund-rising, research management, communication, teaching and academic leadership. These skills that are Instrumental for launching my future independent internationally competitive research career Champ scientifique engineering and technologyindustrial biotechnologymetabolic engineeringengineering and technologychemical engineeringbiochemical engineeringengineering and technologyenvironmental biotechnologybiosensingnatural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Mots‑clés Metabolic engineering Synthetic biology Cell factories High-value compounds Plant specialized metabolites isoprenoids Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2018 - Individual Fellowships Appel à propositions H2020-MSCA-IF-2018 Voir d’autres projets de cet appel Régime de financement MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinateur KOBENHAVNS UNIVERSITET Contribution nette de l'UE € 207 312,00 Adresse NORREGADE 10 1165 Kobenhavn Danemark Voir sur la carte Région Danmark Hovedstaden Byen København Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 207 312,00
