GPCR-based biosensor for synthetic biology applications

Projektinformationen

GPCR-Sensor

ID Finanzhilfevereinbarung: 845835

DOI 10.3030/845835

Projekt abgeschlossen

EK-Unterschriftsdatum 23 April 2019

Startdatum 1 Juni 2020

Enddatum 31 Mai 2022

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 207 312,00

EU-Beitrag € 207 312,00

Koordiniert durch

KOBENHAVNS UNIVERSITET
Denmark

Projektbeschreibung

Neuartige Biosensoren für mikrobielle Produktionsanlagen

Mikrobielle Zellfabriken sind eine wirtschaftliche und nachhaltige Alternative für die Herstellung von Pharmazeutika und Industriechemikalien. Fehlende Hochdurchsatzverfahren, mit denen die Ansammlung der Produkte überwacht werden kann, stellen dabei jedoch ein Hindernis dar. Das EU-finanzierte Projekt GPCR-Sensor möchte mithilfe von G-Protein-gekoppelten Rezeptoren, den Umweltsensoren der Eukaryoten, einen vielseitigen In-vivo-Biosensor entwickeln. Dabei wird das Forschungsteam anhand synthetischer Biologie und eines interdisziplinären Ansatzes einen Biosensor auf Hefebasis für die G-Protein-gekoppelten Rezeptoren entwickeln, mit dem sich Enzyme und Produktionswirte fertigen lassen. Dank der modularen Struktur der Biosensoren können sämtliche G-Protein-gekoppelten Rezeptoren angeschlossen werden, woraus sich ein breites biomedizinisches und ökologisches Einsatzgebiet ergibt.

Ziel

Microbial cell factories are an economical and sustainable alternative for the production of pharmaceuticals, industrial chemicals and other valuable chemicals. However, establishing efficient cell factories requires optimized production strains and efficient biosynthetic enzymes, and involves extensive protein and metabolic engineering to achieve industrially relevant yields. One of the main hurdles frequently encountered in this process is the lack of high-throughput methods for monitoring product accumulation. To overcome this limitation, I will harness G-protein coupled receptors (GPCRs), the unique molecular entities that eukaryotes use to sense their environment, to create a versatile in vivo biosensor. I will apply synthetic biology principles and an interdisciplinary approach to develop a yeast-based GPCR biosensor for the engineering of enzymes and production hosts. However, enzyme engineering is only one of the potential applications of GPCR-sensor. Its modular structure permits plugging in any GPCR, enabling a great variety of other powerful applications including bio-medical applications, such as recognition of pathogen epitopes, molecular diagnostics and drug discovery, or environmental applications, such as determining the presence of pollutants from environmental samples. In the GPCR-sensor project, I will combine my knowledge in natural product biosynthesis and enzymology with training in biochemical engineering and biotechnology to acquire a unique expertise that will help me establish my own unique line of research. Furthermore, the comprehensive professional development program, that is an integral part of GPCR-sensor, includes close mentoring in addition to training in fund-rising, research management, communication, teaching and academic leadership. These skills that are Instrumental for launching my future independent internationally competitive research career

Wissenschaftliches Gebiet

• engineering and technology › industrial biotechnology › metabolic engineering
• engineering and technology › chemical engineering › biochemical engineering
• engineering and technology › environmental biotechnology › biosensing
• natural sciences › biological sciences › synthetic biology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Schlüsselbegriffe

• Metabolic engineering
• Synthetic biology
• Cell factories
• High-value compounds
• Plant specialized metabolites
• isoprenoids

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2018 - Individual Fellowships

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2018

Finanzierungsplan

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Koordinator

KOBENHAVNS UNIVERSITET

• Netto-EU-Beitrag: € 207 312,00
• Adresse: NORREGADE 10 ; 1165 Kobenhavn ; Dänemark
• Region: Danmark › Hovedstaden › Byen København
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 207 312,00