Structure and Cellular Dynamics of the Sarcomere

Project Information

StuDySARCOMERE

Grant agreement ID: 856118

DOI

10.3030/856118

EC signature date 11 December 2019

Start date 1 February 2020

End date 31 January 2027

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 10 936 440,00

EU contribution

€ 10 936 440,00

Coordinated by

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (29)

A nanobody toolbox to investigate localisation and dynamics of Drosophila titins and other key sarcomeric proteins

Author(s): Vincent Loreau; Renate Rees; Eunice HoYee Chan; Waltraud Taxer; Kathrin Gregor; Bianka Mußil; Christophe Pitaval; Nuno Miguel Luis; Pierre Mangeol; Frank Schnorrer; Dirk Görlich
Published in: eLife, Issue 1, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.79343

Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin

Author(s): Zhexin Wang, Michael Grange, Sabrina Pospich, Thorsten Wagner, Ay Lin Kho,Mathias Gautel, Stefan Raunser
Published in: Science, 2022, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.abn1934

Structure of the native myosin filament in the relaxed cardiac sarcomere

Author(s): Tamborrini D, Wang Z, Wagner T, Tacke S, Stabrin M, Grange M, Kho AL, Rees M, Bennett P, Gautel M, Raunser S
Published in: Nature, 2023, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-023-06690-5

Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nano-architecture in flight muscles

Author(s): Pierre Mangeol; Florian Schueder; Eunice HoYee Chan; Renate Rees; Jürgen Schünemann; Ralf Jungmann; Dirk Görlich; Frank Schnorrer
Published in: eLife, Issue 2, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.1101/2022.04.14.488306

Multistate kinetics of the syringe-like injection mechanism of Tc toxins

Author(s): Peter Njenga Ng’ang’a, Julian Folz, Svetlana Kucher, Daniel Roderer, Ying Xu, Oleg Sitsel, Alexander Belyy, Daniel Prumbaum, Ralf Kühnemuth, Tufa E. Assafa, Min Dong, Claus A. M. Seidel, Enrica Bordignon, Stefan Raunser
Published in: Science Advances, Issue 11, 2025, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.adr2019

Location-Dependent Differences in Cardiac and Skeletal Muscle Dysfunction Associated With Truncating Titin ( <i>ttn.2</i> ) Variants

Author(s): Celine F. Santiago, Inken G. Huttner, Ailbhe K. O'Brien, Melissa A.B. Amerudin, Pauline M. Bennett, Jasmina Cvetkovska, Renee Chand, Mark Holt, Gunjan Trivedi, Louis W. Wang, Xiaoping Yang, Kelly A. Smith, Mathias Gautel, Diane Fatkin, Yaniv Hinits
Published in: Circulation Research, Issue 138, 2026, ISSN 0009-7330
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/circresaha.124.325999

Microtubules coordinate mitochondria transport with myofibril morphogenesis during muscle development

Author(s): Jerome Avellaneda, Duarte Candeias, Ana da Rosa Soares, Edgar R. Gomes, Nuno Miguel Luis, Frank Schnorrer
Published in: Developmental Cell, Issue 60, 2025, Page(s) 2962-2975.e5, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2025.06.033

Structural Biochemistry of Muscle Contraction

Author(s): Wang Z, Raunser S
Published in: Ann. Rev. Biochem., 2023, ISSN 0066-4154
Publisher: Annual Reviews, Inc.
DOI: 10.1146/annurev-biochem-052521-042909

Spatial transcriptomics in adult Drosophila reveals new cell types in the brain and identifies subcellular mRNA patterns in muscles

Author(s): Jasper Janssens, Pierre Mangeol, Nikolai Hecker, Gabriele Partel, Katina Spanier, Joy Ismail, Gert Hulselmans, Stein Aerts, Frank Schnorrer
Published in: eLife, 2024, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.92618.1

Myofibril and mitochondria morphogenesis are coordinated by a mechanical feedback mechanism in muscle

Author(s): Jerome Avellaneda, Clement Rodier, Fabrice Daian, Nicolas Brouilly, Thomas Rival, Nuno Miguel Luis, Frank Schnorrer
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-22058-7

The molecular basis for sarcomere organization in vertebrate skeletal muscle

Author(s): Zhexin Wang, Michael Grange, Thorsten Wagner, Ay Lin Kho, Mathias Gautel, Stefan Raunser
Published in: Cell, Issue 184/8, 2021, Page(s) 2135-2150.e13, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2021.02.047

Phalloidin and DNase I-bound F-actin pointed end structures reveal principles of filament stabilization and disassembly

Author(s): Micaela Boiero Sanders, Wout Oosterheert, Oliver Hofnagel, Peter Bieling, Stefan Raunser
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-52251-3

Molecular mechanisms of inorganic-phosphate release from the core and barbed end of actin filaments

Author(s): Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Published in: Nat Struct Mol Biol, 2023, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-023-01101-9

Mechanoresponsive regulation of myogenesis by the force-sensing transcriptional regulator Tono

Author(s): Xu Zhang, Jerome Avellaneda, Maria L. Spletter, Sandra B. Lemke, Pierre Mangeol, Bianca H. Habermann, Frank Schnorrer
Published in: Current Biology, 2024, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2024.07.079

Mechanisms of Sarcomere Assembly in Muscle Cells Inferred from Sequential Ordering of Myofibril Components

Author(s): Francine Kolley, Clara Sidor, Benoit Dehapiot, Frank Schnorrer, Benjamin M. Friedrich
Published in: PRX Life, Issue 2, 2024, ISSN 2835-8279
Publisher: American Physical Society, College Park
DOI: 10.1103/prxlife.2.013002

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.

Author(s): Sabrina Pospich; H. Lee Sweeney; Anne Houdusse; Stefan Raunser
Published in: eLife, Issue 1, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.73724

Titin-dependent biomechanical feedback tailors sarcomeres to specialized muscle functions in insects

Author(s): Vincent Loreau, Wouter H. Koolhaas, Eunice HoYee Chan, Paul De Boissier, Nicolas Brouilly, Sabina Avosani, Aditya Sane, Christophe Pitaval, Stefanie Reiter, Nuno Miguel Luis, Pierre Mangeol, Anne C. von Philipsborn, Jean-François Rupprecht, Dirk Görlich, Bianca H. Habermann, Frank Schnorrer
Published in: Science Advances, Issue 11, 2025, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.ads8716

The physical basis of sarcomere self-assembly

Author(s): Francine Kolley-Köchel, Frank Schnorrer, Benjamin M. Friedrich
Published in: Biophysics Reviews, Issue 6, 2025, ISSN 2688-4089
Publisher: AIP Publishing
DOI: 10.1063/5.0281811

Spatial transcriptomics in the adult Drosophila brain and body

Author(s): Jasper Janssens, Pierre Mangeol, Nikolai Hecker, Gabriele Partel, Katina I Spanier, Joy N Ismail, Gert J Hulselmans, Stein Aerts, Frank Schnorrer
Published in: eLife, Issue 13, 2025, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.92618.3

Mechanobiology of muscle and myofibril morphogenesis.

Author(s): Nuno Miguel Luis, Frank Schnorrer
Published in: Cells & development., Issue 2021 Dec:168:203760, 2021, ISSN 2667-2901
Publisher: [Amsterdam] : Elsevier B.V., [2021]-
DOI: 10.1016/j.cdev.2021.203760

PatternJ: an ImageJ toolset for the automated and quantitative analysis of regular spatial patterns found in sarcomeres, axons, somites, and more

Author(s): Mélina Baheux Blin, Vincent Loreau, Frank Schnorrer, Pierre Mangeol
Published in: Biology Open, Issue 13, 2024, ISSN 2046-6390
Publisher: Biol Open
DOI: 10.1242/bio.060548

Structural insights into actin filament turnover

Author(s): Wout Oosterheert, Micaela Boiero Sanders, Peter Bieling, Stefan Raunser
Published in: Trends in Cell Biology, Issue 35, 2025, Page(s) 893-906, ISSN 0962-8924
Publisher: Elsevier BV
DOI: 10.1016/j.tcb.2024.12.009

Choreography of rapid actin filament disassembly by coronin, cofilin, and AIP1

Author(s): Wout Oosterheert, Micaela Boiero Sanders, Oliver Hofnagel, Peter Bieling, Stefan Raunser
Published in: Cell, Issue 188, 2025, Page(s) 6845-6860.e27, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2025.09.016

Tension-driven multi-scale self-organisation in human iPSC-derived muscle fibers

Author(s): Achyuth Acharya; Qiyan Mao; Alejandra Rodríguez-delaRosa; Fabio Marchiano; Benoit Dehapiot; Ziad Al Tanoury; Jyoti Rao; Margarete Díaz-Cuadros; Arian Mansur; Erica Wagner; Claire Chardes; Vandana Gupta; Pierre-François Lenne; Bianca H Habermann; Olivier Theodoly; Olivier Pourquié; Frank Schnorrer
Published in: eLife, Issue 1, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.76649

A comprehensive framework for the interpretation of TTN missense variants

Author(s): Maria Francesca Di Feo, Martin Rees, Victoria Lillback, Ay Lin Kho, Angelina Meybatova, Mark Holt, Heinz Jungbluth, Francesco Muntoni, Giovanni Baranello, Anna Sarkozy, Chiara Fiorillo, Serena Baratto, Claudio Bruno, Monica Traverso, Michele Iacomino, Marina Pedemonte, Noemi Brolatti, Francesca Faravelli, Federico Zara, G. M. Luana Mandarà, Alan H. Beggs, Casie A. Genetti, Pamela Barraza-Flores,
Published in: Genome Medicine, 2026, ISSN 1756-994X
Publisher: BioMed Central
DOI: 10.1186/s13073-026-01605-1

TITINdb2—expanding annotation and structural information for protein variants in the giant sarcomeric protein titin

Author(s): Timir Weston, Joseph Ng, Oriol Gracia Carmona, Mathias Gautel, Franca Fraternali
Published in: Bioinformatics Advances, Issue 5, 2025, ISSN 2635-0041
Publisher: Oxford University Press (OUP)
DOI: 10.1093/bioadv/vbaf062

Molecular mechanism of actin filament elongation by formins

Author(s): Wout Oosterheert, Micaela Boiero Sanders, Johanna Funk, Daniel Prumbaum, Stefan Raunser, Peter Bieling
Published in: Science, Issue 384, 2024, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.adn9560

Structural Basis of actin filament assembly and aging

Author(s): Wout Oosterheert, Björn U. Klink, Alexander Belyy, Sabrina Pospich,Stefan Raunser
Published in: Nature, 2022, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-022-05241-8

Muscle growth by sarcomere divisions

Author(s): Clement Rodier, Ian D. Estabrook, Eunice HoYee Chan, Gavin Rice, Vincent Loreau, Stefan Raunser, Dirk Görlich, Benjamin M. Friedrich, Frank Schnorrer
Published in: Science Advances, Issue 11, 2025, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.adw9445

Non-peer reviewed articles (1)

Nucleoporin-binding nanobodies that either track or inhibit nuclear pore complex assembly

Author(s): Mireia Solà Colom, Zhenglin Fu, Thomas Güttler, Sergei Trakhanov, Vasundara Srinivasan, Kathrin Gregor, Tino Pleiner & Dirk Görlich
Published in: bioRxiv, 2023, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.09.12.557426