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Next Generation Instrument for In-Depth Analysis of Mitochondrial Fitness

Resultado final

Intermediate Communication Plan

Report containing updated communication strategy and activities performed during Year 1 of the project.

Final Communication Plan

Report containing the final communication plan and all the activities and results performed during Year 1 and Year 2 of the project

Final SOPs and User’s Manual for each module

A compiled list of SOPs and protocols in the User Manual that will be posted online and accompany the NextGenO2k device

Website

Updated website with a dedicated section for NextGen-O2k Phase 2 project.

Publicaciones

Comparable respiratory activity in attached and suspended human fibroblasts.

Autores: Zdrazilova L, Hansikova H, Gnaiger E
Publicado en: MitoFit Preprints, 2021.7, 2021
Editor: Oroboros Instruments

O2k-FluoRespirometer manual

Autores: Gnaiger E, Komlódi T, Schmitt S, Cecatto C, Gradl L, Gradl P
Publicado en: Mitochondrial Physiology Network, 22.11(v9), 2021
Editor: Oroboros Instruments

Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation

Autores: Cardoso LHD and Tindle-Solomon L
Publicado en: O2k-brief, 21/07/2020, 2020
Editor: Oroboros Instruments

Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 24/06/2020, 2020
Editor: Oroboros Instruments

Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology

Autores: Falaye T, Sobotka O and Tindle-Solomon L
Publicado en: O2k-brief, 30/07/2020, 2020
Editor: Oroboros Instruments

NextGen-O2k: PB-Module manual

Autores: Di Marcello M, Merth A, Doerrier C, Schmitt S, Schwaninger H, Walter-Vracevic M, Gradl P, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 26.11, 2021
Editor: Oroboros Instruments

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8.

Autores: Gnaiger E
Publicado en: Mitochondrial Physiology Network, 06.03, 2021
Editor: Oroboros Instruments

Service of the polarographic oxygen sensor OroboPOS.

Autores: Gnaiger E
Publicado en: Mitochondrial Physiology Network, 19.18B(v9), 2020
Editor: Oroboros Instruments

DatLab-guide through the menus

Autores: Gnaiger E, Cecatto C, Gradl L
Publicado en: Mitochondrial Physiology Network, 26.06, 2021
Editor: Oroboros Instruments

O2k-Chamber cleaning SOP and Integrated Suction System (ISS).

Autores: Di Marcello M, Komlodi T, Schmitt S, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 19.03(v7), 2021
Editor: Oroboros Instruments

O2k-MultiSensor system with ion selective electrodes (ISE).

Autores: Komlodi T, Schmitt S, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 15.03(v9), 2021
Editor: Oroboros Instruments

Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity

Autores: Cecatto C and Tindle-Solomon L
Publicado en: O2k-brief, 27/04/2021, 2021
Editor: Oroboros Instruments

O2k-checklist: get started with an O2k-experiment

Autores: Doerrier C, Schmitt S, Cecatto C, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 20.04(v5), 2021
Editor: Oroboros Instruments

Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts

Autores: Gnaiger E
Publicado en: MitoFit Preprints, 2021.8, 2021
Editor: Oroboros Instruments

Doxorubicin-transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 30/06/2021, 2021
Editor: Oroboros Instruments

Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration

Autores: Cardoso LHD, Doerrier C, Gnaiger
Publicado en: Bioenergetics Communications, 2021.1, 2020
Editor: Oroboros Instruments

Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth

Autores: Cecatto C and Tindle-Solomon L
Publicado en: O2k-brief, 18/11/2020, 2020
Editor: Oroboros Instruments

Laboratory protocol: isolation of mouse heart mitochondria

Autores: Komlodi T, Cardoso LHD, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 20.068v2), 2021
Editor: Oroboros Instruments

High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations

Autores: Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E
Publicado en: MitoFit Preprints, 2020
Editor: Oroboros Instruments

Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration.

Autores: Cardoso LHD, Doerrier C, Gnaiger E
Publicado en: MitoFit Preprints, 2021.1, 2021
Editor: Oroboros Instruments

Effects of ultramarathon running on mitochondrial function of platelets and oxidative stress parameters: a pilot study

Autores: Cecatto C and Tindle-Solomon L
Publicado en: O2k-brief, 16/09/2021, 2021
Editor: Oroboros Instruments

O2k-Specifications for respirometry and comprehensive OXPHOS analysis

Autores: Gnaiger E
Publicado en: Mitochondrial Physiology Network, 18.10(09), 2019, Page(s) 1-8
Editor: Oroboros Instruments

O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux

Autores: Fasching M, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 14.06(08), 2020, Page(s) 1-16
Editor: Oroboros Instruments

NextGen-O2k: Q-Module Manual

Autores: Komlodi T, Cardoso LHD, Gollner M, Merth A, Hunger M, Iglesias-Gonzalez J, Doerrier C, Tindle-Solomon L, Schwaninger H, Walter-Vracevic M, Gradl P, Moore AL, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 24.12(02), 2020, Page(s) 1-21
Editor: Oroboros Instruments

DatLab 8.0: Cyclic voltammetry manual

Autores: Komlodi T, Cardoso LHD, Haider M, Tindle-Solomon L, Iglesias-Gonzalez J, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 24.16(01), 2019, Page(s) 1-10
Editor: Oroboros Instruments

Oxygen flux analysis with DatLab 7.4

Autores: Cardoso LHD, Antunes D, Iglesias-Gonzalez J, Komlodi T, Doerrier C, Garcia-Souza LF, Gnaiger E, Sobotka O
Publicado en: Mitochondrial Physiology Network, 24.06(02), 2020, Page(s) 1-11
Editor: Oroboros Instruments

Hydrogen peroxide flux analysis using Amplex Ultra Red assay in MiR05-Kit with DatLab 7.4

Autores: Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 24.10(02), 2020, Page(s) 1-6
Editor: Oroboros Instruments

Data analysis of mitochondrial membrane potential estimation using safranin

Autores: Komlodi T, Fasching M, Antunes D, Iglesias-Gonzalez J, Sobotka O, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 24.08(02), 2020, Page(s) 1-8
Editor: Oroboros Instruments

Data analysis of mitochondrial membrane potential estimation using various fluorescence dyes

Autores: Komlodi T, Antunes D, Iglesias-Gonzalez J, Sobotka O, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 24.09(02), 2020, Page(s) 1-5
Editor: Oroboros Instruments

Mitochondrial respiratory states and rates

Autores: Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, Ahn B, Alencar MB, Ali SS, Almeida A, Alton L, Alve sMG, Amati F, Amoedo ND, Amorim R, Andreadou I, Antunes D, Aragó M, Aral C, Arandarčikaitė O,Arias Reyes C, Armand AS, Arnould T, Avram VF, Bailey DM, Bairam A, Bajpeyi S, Bajzikova M, Bakker BM, Banni A, Bardal T, Barlow J, Bastos Sant'
Publicado en: MitoFit Preprint Archives, 2019
Editor: Oroboros Instruments

Mitochondrial physiology

Autores: Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, Ahn B, Alencar MB, Ali SS, Almeida A, Alton L, Alve sMG, Amati F, Amoedo ND, Amorim R, Andreadou I, Antunes D, Aragó M, Aral C, Arandarčikaitė O,Arias Reyes C, Armand AS, Arnould T, Avram VF, Bailey DM, Bairam A, Bajpeyi S, Bajzikova M, Bakker BM, Banni A, Bardal T, Barlow J, Bastos Sant'
Publicado en: Bioenergetics Communications, 2020
Editor: Oroboros Instruments

Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers

Autores: Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM
Publicado en: MitoFit Preprint Archives, 2019
Editor: Oroboros Instruments

Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism

Autores: Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M
Publicado en: MitoFit Preprint Archives, 2019
Editor: Oroboros Instruments

Generating reference values on mitochondrial respiration in permeabilized muscle fibers

Autores: Chabi Béatrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wüst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM
Publicado en: MitoFit Preprint Archives, 2019
Editor: Oroboros Instruments

Commitment to reproducibility in targeting mitochondrial respiratory control: basic and advanced applications of the O2k-FluoRespirometer

Autores: Doerrier C, Di Marcello M, Gnaiger E
Publicado en: 2020
Editor: Foundation for Translational Myology

Endogenous quinones sustain a moderate NADH oxidation by Complex I during anoxia

Autores: Ravasz D, Bui D, Kitayev A, Greenwood B, Hill C, Komlodi T, Doerrier C, Ozohanics O, Moore AL, Gnaiger E, Kiebish M, Kolev K, Seyfried TN, Willis WT, Narain N, Adam-Vizi V, Chinopoulos C
Publicado en: 2019
Editor: Mitochondrial Physiology Network

Succinate dehydrogenase is a source of reactive oxygen species in plants and regulates development and stress responses

Autores: Huete-Ortega M
Publicado en: O2k-brief, 2019-06-05, 2019
Editor: Oroboros Instruments

Regulation of ATP production: dependence on calcium concentration and respiratory state

Autores: Cardoso L
Publicado en: O2k-brief, 2019-06-12, 2019
Editor: Oroboros Instruments

Mitochondrial respiration in peripheral blood mono nuclear cells correlates with depressive sub-symptoms and severity of major depression

Autores: Garcia-Souza LF
Publicado en: O2k-brief, 2019-06-19, 2019
Editor: Oroboros Instruments

Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart

Autores: Gnaiger E and Iglesias-Gonzalez J
Publicado en: O2k-brief, 2019-06-26, 2019
Editor: Oroboros Instruments

High intensity exercise inhibits carnitine palmoyltranferase-I sensitivity to L-carnitine

Autores: Krumschnable G
Publicado en: O2k-brief, 2019-07-02, 2019
Editor: Oroboros Instruments

Rapid kinetics of changes in oxygen consumption rate in thrombin-stimulated platelets measured by high-resolution respirometry

Autores: Garcia-Souza LF
Publicado en: O2k-brief, 2019-07-09, 2019
Editor: Oroboros Instruments

Direct effects of phenformin on metabolism/bioenergetics and viability of SH-SY5Y neuroblastoma cells

Autores: Bastos AC
Publicado en: O2k-brief, 2019
Editor: Oroboros Instruments

Selective disruption of respiratory supercomplexes as a new strategy to supress Her2high breast cancer

Autores: Bastos AC
Publicado en: O2k-brief, 2019-07-24, 2019
Editor: Oroboros Instruments

Endogenous myoglobin in breast cancer is hypoxia-inducible by alternate transcription and functions to impair mitochondrial activity: a role in tumor suppression?

Autores: Iglesias-Gonzalez J
Publicado en: O2k-brief, 2019-07-31, 2019
Editor: Oroboros Instruments

6-Hydroxidopamine induces different mitochondrial bioenergetics response in brain regions of rat

Autores: Iglesias-Gonzalez J
Publicado en: O2k-brief, 2019-08-07, 2019
Editor: Oroboros Instruments

Non-canonical BAD activity regulates breast cancer cells and tumor growth via 14-3-3 binding and mitochondrial metabolism

Autores: Tindle-Solomon L
Publicado en: O2k-brief, 2019-10-25, 2019
Editor: Oroboros Instruments

Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption

Autores: Tindle-Solomon L
Publicado en: O2k-brief, 2019-11-28, 2019
Editor: Oroboros Instruments

Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices

Autores: Tindle-Solomon L
Publicado en: O2k-brief, 2019-11-12, 2019
Editor: Oroboros Instruments

Inhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis

Autores: Antunes D and Tindle-Solomon L
Publicado en: O2k-brief, 2020-01-20, 2020
Editor: Oroboros Instruments

The metabolite BH4 controls T cell proliferation in autoimmunity and cancer

Autores: Garcia-Souza LF and Tindle-Solomon L
Publicado en: O2k-brief, 2020-02-04, 2020
Editor: Oroboros Instruments

Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart

Autores: Cecatto C and Tindle-Solomon L
Publicado en: O2k-brief, 2020-03-18, 2020
Editor: Oroboros Instruments

Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain

Autores: Antunes D and Tindle-Solomon L
Publicado en: O2k-brief, 2020-03-05, 2020
Editor: Oroboros Instruments

Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress

Autores: Antunes D and Tindle-Solomon L
Publicado en: O2k-brief, 2020-04-03, 2020
Editor: Oroboros Instruments

Respiratory chain signaling is essential for adaptive remodeling following cardiac ischaemia

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 2020-05-15, 2020
Editor: Oroboros Instruments

OXPHOS remodeling n high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation

Autores: Bastos AC and Tindle-Solomon L
Publicado en: O2k-brief, 2020-05-28, 2020
Editor: Oroboros Instruments

Peripheral mitochondrial dysfunction in Alzheimer’s disease: focus on lymphocytes.

Autores: Garcia-Souza LF and Tindle-Solomon L
Publicado en: O2k-brief, 2020-04-16, 2020
Editor: Oroboros Instruments

High-Fat diet activates liver iPLA2γ generating eicosanoids that mediate metabolic stress

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 15/06/2021, 2021
Editor: Oroboros Instruments

Platelet mitochondrial respiration, endogenous coenzyme Q10 and oxidative stress in patients with chronic kidney disease

Autores: Falaye T, Sobotka O and Tindle-Solomon L
Publicado en: O2k-brief, 29/05/2020, 2020
Editor: Oroboros Instruments

NextGen-O2k manual

Autores: Gnaiger E, Cardoso LHD, Cecatto C, Niedenzu W, Haider M, Gollner M, Merth A, Schwaninger H, Walter-Vracevic M, Gradl P
Publicado en: Mitochondrial Physiology Network, 26.13, 2021
Editor: Oroboros Instruments

Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4

Autores: Cardoso LHD, Komlodi T, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 26.10, 2021
Editor: Oroboros Instruments

NextGen-O2k: NADH-Module manual

Autores: Doerrier C, Schwaninger H, Walter-Vracevic M, Haider M, Niedenzu W, Komlódi T, Gradl P, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 26.12, 2021
Editor: Oroboros Instruments

Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice

Autores: Cecatto C and Tindle-Solomon L
Publicado en: O2k-brief, 18/09/2020, 2020
Editor: Oroboros Instruments

Adaptation of mitochondrial substrate flux in a mouse model of nonalcoholic fatty liver disease

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 04/01/2021, 2021
Editor: Oroboros Instruments

An mtDNA mutant mouse demonstrates that mitochondrial deficiency can result in autism endophenotypes

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 29/03/2021, 2021
Editor: Oroboros Instruments

Ex vivo mitochondrial respiration parallels biochemical response to ibrutinib in CLL cells

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 16/08/2021, 2021
Editor: Oroboros Instruments

Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry

Autores: Went N, Di Marcello M, Gnaiger E
Publicado en: MitoFit Preprints, 2021.5, 2021
Editor: Oroboros Instruments

The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures

Autores: Gnaiger E
Publicado en: MitoFit Preprints, 2021.4(v2), 2021
Editor: Oroboros Instruments

Colon cancer cell differentiation by sodium butyrate modulates metabolic plasticity of Caco-2 cells via alteration of phosphotransfer network

Autores: Komlodi T and Tindle-Solomon L
Publicado en: O2k-brief, 09/06/2021, 2019
Editor: Oroboros Instruments

Titration-Injection microPump TIP2k manual.

Autores: Gradl L, Gradl P, Gnaiger E
Publicado en: Mitochondrial Physiology Network, 12.10(v9), 2021
Editor: Oroboros Instruments

Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria

Autores: Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E
Publicado en: MitoFit Preprints, 2021.2(v4), 2021
Editor: Oroboros Instruments

Mitochondrial respiration of platelets: comparison of isolation methods.

Autores: Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O
Publicado en: MitoFit Preprints, 2021.6, 2021
Editor: Oroboros Instruments

Mitochondrial pathways and respiratory control

Autores: Gnaiger E
Publicado en: Bioenergetics Communicatoins, 2020.2, 2020, ISBN 978-3-9504814-2-6
Editor: Bioenergetics Communicatoins